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Plant Growth Regulation

, Volume 53, Issue 1, pp 65–73 | Cite as

Arsenic-induced root growth inhibition in mung bean (Phaseolus aureus Roxb.) is due to oxidative stress resulting from enhanced lipid peroxidation

  • Harminder Pal Singh
  • Daizy R. Batish
  • Ravinder Kumar Kohli
  • Komal Arora
Original Paper

Abstract

Arsenic (As) toxicity and its biochemical effects have been mostly evaluated in ferns and a few higher plants. In this study, we investigated the effect of As (10.0 and 50.0 μM) on seedling growth, root anatomy, lipid peroxidation (malondialdehyde and conjugated dienes), electrolyte leakage, H2O2 content, root oxidizability and the activities of antioxidant enzymes in mung bean (Phaseolus aureus Roxb.). Arsenic significantly enhanced lipid peroxidation (by 52% at 50.0 μM As), electrolyte leakage and oxidizability in roots. However, there was no significant change in H2O2 content. Arsenic toxicity was associated with an increase in the activities of superoxide dismutase (SOD), guaiacol peroxidase (GPX) and glutathione reductase (GR). In response to 50.0 μM As, the activities of SOD and GR increased by over 60% and 90%, respectively. At 10.0 μM As, the activity of ascorbate peroxidase (APX) increased by 83%, whereas at 50.0 μM it declined significantly. The catalase (CAT) activity, on the other hand, decreased in response to As exposure, and it corresponded to the observed decrease in H2O2 content. We conclude that As causes a reduction in root elongation by inducing an oxidative stress that is related to enhanced lipid peroxidation, but not to H2O2 accumulation.

Keywords

Arsenic Antioxidant enzymes Conjugated dienes Electrolyte leakage Lipid peroxidation Oxidative damage Phaseolus aureus Root oxidizability 

Abbreviations

APX

Ascorbate peroxidase

As

Arsenic

CAT

Catalase

CD

Conjugated diene

DW

Dry weight

EL

Electrolyte leakage

FW

Fresh weight

GPX

Guaiacol peroxidase

GR

Glutathione reductase

GSH

Glutathione reduced

GSSG

Glutathione oxidized

H2O2

Hydrogen peroxide

LP

Lipid peroxidation

MDA

Malondialdehyde

NADPH

Nicotinamide adenine dinucleotide phosphate reduced

NBT

Nitro blue tetrazolium

RO

Root oxidizability

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TBARS

Thiobarbituric acid reactive substances

TCA

Trichloroacetic acid

TTC

2,3,5-Triphenyl tetrazolium chloride

Notes

Acknowledgement

Komal Arora is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi for financial assistance in the form of a research fellowship.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Harminder Pal Singh
    • 1
  • Daizy R. Batish
    • 2
  • Ravinder Kumar Kohli
    • 1
    • 2
  • Komal Arora
    • 2
  1. 1.Centre for Environment and Vocational StudiesPanjab UniversityChandigarhIndia
  2. 2.Botany DepartmentPanjab UniversityChandigarhIndia

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