Advertisement

Plant Growth Regulation

, Volume 52, Issue 2, pp 151–159 | Cite as

Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements

  • Ya-Li Wang
  • Xiao-Dong Wang
  • Bing Zhao
  • Yu-Chun Wang
Original Paper

Abstract

Adventitious shoots induced from maca calli on induction media without rare earth elements (REE) had higher water content and lower soluble protein concentration when compared with shoots sprouted from maca seeds. Due to lower activities of antioxidative enzymes, there were higher concentrations of H2O2 and malonyldialdehyde (MDA) in adventitious shoots than those in seed shoots. When La3+, Ce3+ and Nd3+ (0.04 mM to 0.1 mM) were added to induction media, induction rates of the adventitious shoots were only affected slightly, but hyperhydricity rates were significantly reduced. La3+, Ce3+ or Nd3+ enhanced the activities of antioxidative enzymes in adventitious shoots, e.g. peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR). When the concentrations of La3+, Ce3+ and Nd3+ were 0.1 mM, the oxygen stress in adventitious shoots was decreased to levels similar to seed shoots, where most adventitious shoots grew normally.

Keywords

Adventitious shoots Antioxidative enzymes Hyperhydricity Maca (Lepidium meyenii) Rare earth elements 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

DPPH

1,1-diphenyl-2-picrylhydarazyl

GR

Glutathione reductase

MDA

Malonyldialdehyde,

MDHAR

Monodehydroascorbate reductase

MS

Murashige and Skoog’s (1962) medium

NBT

Nitrotetrazolium blue tetrazolium

POD

Peroxidase

REE

Rare earth elements

SOD

Superoxide dismutase

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

References

  1. Azevedo Neto AD, Prisco JT, Enéas-Filho J, Abreu CEB, Gomes-Filho E (2006) Effect of salt stress on antioxidative enzymes and lipid perosidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot 56:87–94CrossRefGoogle Scholar
  2. Beyer WF, Fridovic I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in condition. Anal Biochem 161:559–566PubMedCrossRefGoogle Scholar
  3. Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org 64:145–157CrossRefGoogle Scholar
  4. Chen J, Ziv M (2001) The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep 20:22–27CrossRefGoogle Scholar
  5. Cicero AF, Piacente S, Plaza A, Sala E, Arletti R, Pizza C (2002) Hexanic Maca extract improves rat sexual performance more effectively than methanolic and chloroformic Maca extracts. Andrologia 34:177–179PubMedCrossRefGoogle Scholar
  6. Dily FL, Huault C, Gaspar T, Billard JP (1993) Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)? Plant Cell Tiss Org 35:69–74CrossRefGoogle Scholar
  7. Franck T, Gaspar T, Kevers C, Penel C, Dommes J, Hausman JF (2001) Are hyperhydric shoots of Prunus avium L. energy deficient? Plant Sci 160:1145–1151Google Scholar
  8. Franck T, Kevers C, Gaspar T, Dommes J, Deby C, Greimers R, Serteyn D, Deby-Dupont G (2004) Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response. Plant Physiol Biochem 42:519–527PubMedCrossRefGoogle Scholar
  9. Gonzales GF, Cordova A, Vega K, Chung A, Villena A, Gonez C, Castillo S (2002) Effect of Lepidium meyenii (MACA) on sexual desire and its absent relationship with serum testosterone levels in adult healthy men. Andrologia 34:367–372PubMedCrossRefGoogle Scholar
  10. Guo CR, Shi RX, Wang JS, Cheng YX (2000) Protective effect of Cerium on Chloroplast in wheat seeding. J Chin Rare Earths Soc 18:367–370 [In China]Google Scholar
  11. Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395Google Scholar
  12. Hu QH, Ye ZJ (1996) Physiological effects of rare-earth elements on plants. Plant Physiol Commun 32:296–300 [In China]Google Scholar
  13. Kevers C, Franck T, Strasser RJ, Dommes J, Gaspar T (2004) Hyperhydricity of micropropagated shoots: a typically stress-induced change of physiological state. Plant Cell Tiss Org 77:181–191CrossRefGoogle Scholar
  14. Li HS (eds) (2000) Principles and techniques of plant physiological biochemical experiment. Higher Education Press, BeijingGoogle Scholar
  15. Lee KJ, Dabrowski K, Sandoval M, Miller MJS (2005) Activity-guided fractionation of phytochemicals of maca meal, their antioxidant activities and effects on growth, feed utilization, and survival in rainbow trout (Oncorhynchus mykiss) juveniles. Aquaculture 244:293–301CrossRefGoogle Scholar
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  17. Olmos E, Piqueras A, Martínez-Solano JR, Hellín E (1997) The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation. Plant Sci 130:97–105CrossRefGoogle Scholar
  18. Peng A, Pang X (2002) The free radical mechanism of rare earth elements in anti-adversity for plants. Environ Chem 21:313–317 [In China]Google Scholar
  19. Pütter J (1974) Peroxidase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, p 673–684Google Scholar
  20. Rea J (1992) Raíces andinas: maca. In: Bermejo H, León JE (eds) Cultivos marginados, otra perspectiva de 1492. FAO Publ, p 163–166Google Scholar
  21. Saher S, Fernándea-García N, Piqueras A, Hellín E, Olmos E (2005) Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress? Plant Physiol Biochem 43:573–582PubMedCrossRefGoogle Scholar
  22. Türkan I, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231CrossRefGoogle Scholar
  23. Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated been plants protective role of exogenous polymines. Plant Sci 151:59–66CrossRefGoogle Scholar
  24. Wang X, Shi GX, Xu QS, Wang CT (2004) Toxic effects of lanthamum, cerium, chromium and zinc on pata mogeton malaianus. J Chin Rare Earths Soc 22:682–686 [In China]Google Scholar
  25. Wang JS, Guo CR, Cheng YX (1997) Mechanism of cerium ion clearing superoxide radical. J Chin Rare Earths Soc 15:151–154 [In China]Google Scholar
  26. Yuan XF, Wang Q, Zhao B, Wang YC (2002) Improved cell growth and total flavonoids of Saussurea medusa on solid culture medium supplemented with rare earth elements. Biotechnol Lett 24:1889–1892CrossRefGoogle Scholar
  27. Zheng BL, He K, Kim CH, Rogers L, Shao Y, Huang ZY, Lu Y, Yan SJ, Qien LC, Zheng QY (2000a) Effect of a lipidic extract from Lepidium meyenii on sexual behavior in mice and rats. Urology 55:598–602PubMedCrossRefGoogle Scholar
  28. Zheng HL, Zhao ZQ, Zhang CG, Feng JC, Su MJ (2000b) Advances in mechanism research of rare earth biological effect. Chin Rare Earths 21:55–60 [In China]Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Ya-Li Wang
    • 1
    • 2
  • Xiao-Dong Wang
    • 1
  • Bing Zhao
    • 1
  • Yu-Chun Wang
    • 1
  1. 1.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingP.R. China
  2. 2.Graduate School of Chinese Academy of SciencesBeijingP.R. China

Personalised recommendations