Plant Growth Regulation

, Volume 52, Issue 2, pp 151–159 | Cite as

Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements

  • Ya-Li Wang
  • Xiao-Dong Wang
  • Bing Zhao
  • Yu-Chun Wang
Original Paper


Adventitious shoots induced from maca calli on induction media without rare earth elements (REE) had higher water content and lower soluble protein concentration when compared with shoots sprouted from maca seeds. Due to lower activities of antioxidative enzymes, there were higher concentrations of H2O2 and malonyldialdehyde (MDA) in adventitious shoots than those in seed shoots. When La3+, Ce3+ and Nd3+ (0.04 mM to 0.1 mM) were added to induction media, induction rates of the adventitious shoots were only affected slightly, but hyperhydricity rates were significantly reduced. La3+, Ce3+ or Nd3+ enhanced the activities of antioxidative enzymes in adventitious shoots, e.g. peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR). When the concentrations of La3+, Ce3+ and Nd3+ were 0.1 mM, the oxygen stress in adventitious shoots was decreased to levels similar to seed shoots, where most adventitious shoots grew normally.


Adventitious shoots Antioxidative enzymes Hyperhydricity Maca (Lepidium meyenii) Rare earth elements 



Ascorbate peroxidase






Glutathione reductase




Monodehydroascorbate reductase


Murashige and Skoog’s (1962) medium


Nitrotetrazolium blue tetrazolium




Rare earth elements


Superoxide dismutase


Thiobarbituric acid


Trichloroacetic acid


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Ya-Li Wang
    • 1
    • 2
  • Xiao-Dong Wang
    • 1
  • Bing Zhao
    • 1
  • Yu-Chun Wang
    • 1
  1. 1.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingP.R. China
  2. 2.Graduate School of Chinese Academy of SciencesBeijingP.R. China

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