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Plant Growth Regulation

, Volume 49, Issue 2–3, pp 157–165 | Cite as

Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch)

  • Yan PanEmail author
  • Li Jun Wu
  • Zeng Liang Yu
Original Paper

Abstract

The effects of salinity and drought on the antioxidative system (SOD, POD, CAT) were studied in liquorice seedlings (Glycyrrhiza uralensis Fisch). The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. The activities of SOD and POD were up-regulated by salt and drought stress, while CAT activity decreased. An additional MnSOD isoenzyme was detected in liquorice subjected to 2%NaCl stress. The data also showed that although the activity of SOD was differentially influenced by drought and salinity, the changes of antioxidant enzyme activities subjected to drought stress follow a pattern similar to that subjected to salt stress, indicating that similar defensive systems might be involved in the oxidative stress injury in liquorice.

Keywords

Liquorice seedlings Salt stress Drought stress Antioxidative enzymes MnSOD 

Abbreviations

ROS

Reactive oxygen species

SOD

Superoxide dismutase

CAT

Catalase

POD

Peroxidase

MDA

Malondialdehyde

RWC

Relative water content

PVP

Polyvinylpyrrolidon

EDTA

Ethylenediaminetetraacetic acid

CK

Control

TEMED

N,N,N′,N′-Tetramethylethylenediamine

PEG

Polyethylene glycol

PBS

Phosphate buffer saline

NBT

Nitroblue tetrazolium

dw

Distilled water

Notes

Acknowledgements

This research was supported by National High-Tech Research and Development Plan of China under grant No. 2002AA327070 and No.2004AA326060.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Key Laboratory of Ion Beam Bio-engineeringInstitute of Plasma Physics, Chinese Academy of SciencesAnhuiChina
  2. 2.Institute of Radiation MedicineFudan UniversityShanghaiChina

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