Plant Growth Regulation

, Volume 48, Issue 2, pp 137–144 | Cite as

Thermotolerance and Related Antioxidant Enzyme Activities Induced by Heat Acclimation and Salicylic Acid in Grape (Vitis vinifera L.) Leaves

Article

Abstract

Thermotolerance and related antioxidant enzyme activities induced by both heat acclimation and exogenous salicylic acid (SA) application were studied in grapevine (Vitis vinifera L. cv. Jingxiu). Heat acclimation and exogenous SA application induced comparable changes in thermotolerance, ascorbic acid (AsA), glutathione (GSH), and hydrogen peroxide (H2O2) concentrations, and in activities of the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), ascorbic peroxidase (APX) and catalase (CAT) in grape leaves. Within 1 h at 38 °C, free SA concentration in leaves rose from 3.1 μg g−1 FW to 19.1 μg g−1 FW, then sharply declined. SA application and heat acclimation induced thermotolerance were related to changes of antioxidant enzyme activities and antioxidant concentration, indicating a role for endogenous SA in heat acclimation in grape leaves.

Keywords

Antioxidant enzymes H2O2 Heat acclimation Salicylic acid Thermotolerance Vitis vinifera 

Abbreviation

APX

ascorbate peroxidase

AsA

ascorbic acid

CAT

catalase

GR

glutathione reductase

GSH

glutathione

POD

peroxidase

SA

salicylic acid

SOD

superoxide dismutase

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Banzet, N., Richaud, C., Deveaux, Y., Kazmaier, M., Gagnon, J., Triantaphylides, C. 1998Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cellsPlant J.13519527CrossRefPubMedGoogle Scholar
  2. Beers, P.F., Sizer, I.W. 1952A spectrophotometric assay for measuring the breakdown of hydrogen peroxide by catalaseJ. Biol. Chem.195133138PubMedGoogle Scholar
  3. Borsani, O., Valpuesta, V., Botella, M.A. 2001Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis plantsPlant Physiol.12610241030CrossRefPubMedGoogle Scholar
  4. Bowler, C., Camp, W., Montagu, M., Inze, D. 1994Superoxide dismutase in plantsCrit. Rev. Plant Sci.13199218Google Scholar
  5. Chen, H.H., Shen, Z.Y., Li, P.H. 1982Adaptability of crop plants to high temperature stressCrop Sci.22719725Google Scholar
  6. Chen, Z., Riciglian, J.R., Klessig, D.F. 1993Purification and characterization of a soluble salicylic acid binding protein from tobaccoProc. Natl. Acad. Sci. USA9095339537PubMedGoogle Scholar
  7. Conrath, U., Silva, H., Klesslg, D.F. 1997Protein dephosphorylation mediates salicylic acid-induced expression of PR-1 genes in tobaccoPlant J.11747757CrossRefGoogle Scholar
  8. Dat, J.F., Lopez-Delgado, H., Foyer, C.H., Scott, I.M. 1998aParallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard plantsPlant Physiol.11613511357CrossRefGoogle Scholar
  9. Dat, J.F., Foyer, C.H., Scott, I.M. 1998bChange in salicylic acid and antioxidants during induced thermotolerance in mustard plantsPlant Physiol.11814451456CrossRefGoogle Scholar
  10. Ding, C.K., Wang, C.Y., Gross, K.C., Smith, D.L. 2002Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to cold injury in tomato fruitPlanta214895901CrossRefPubMedGoogle Scholar
  11. Duner, J., Shah, J., Klessig, D.F. 1997Salicylic acid and disease resistance in plantsTrends Plant Sci.7266274Google Scholar
  12. Foster, J.G., Hess, J.L. 1980Response of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygenPlant Physiol.66482487Google Scholar
  13. Foyer, C.H., Rowell, J., Walker, D. 1983Measurements of the ascorbate content of spinach leaf protoplasts and chloroplasts during illuminationPlanta157239244CrossRefGoogle Scholar
  14. Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M. 1997Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signallingPhysiol. Plant.100241254CrossRefGoogle Scholar
  15. Gecheva, T., Gadjeva, I., Breusegemb, F., Inzéb, D., Dukiandjieva, S., Tonevaa, V., Minkova, I. 2002Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymesCell. Mol. Life Sci.59708714Google Scholar
  16. Griffiths, O.W. 1980Determination of glutathione and glutathione disulphide using glutathione reductase and 2-vinypyridineAnal. Biochem.106207212Google Scholar
  17. Hammerschmidt, R., Nuckles, E.M., Kuc, J. 1982Association of enhanced peroxide activity with induced systemic resistance of cucumber to Colletotrichum lagenariumPhysiol. Plant Pathol.207382Google Scholar
  18. Janda, T., Szalai, G., Tari, L., Paldi, E. 1999Hydroponic treatment with salicylic acid decreases the effects of cold injury in maize (Zea mays L.) plantsPlanta208175180CrossRefGoogle Scholar
  19. Kawano, T., Sahashi, N., Takahashi, K., Uozumi, N., Muto, S. 1998Salicylic acid induces extracellular superoxide generation followed by an increase in cytosolic calcium ion in tobacco suspension culture: the earliest events in salicylic acid signal transductionPlant Cell Physiol.39721730Google Scholar
  20. Karpinski, S., Escobar, C., Karpinska, B., Creissen, G., Mullineaux, P.M. 1997Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stressPlant Cell9627640CrossRefPubMedGoogle Scholar
  21. Kraus, T.E., Fletcher, R.A. 1994Paclobutrazol protects wheat plants from heat and paraquat injury. Is detoxification of active oxygen involved?Plant Cell Physiol.354552Google Scholar
  22. Kubo, A., Saji, H., Tanaka, K., Kondo, N. 1995Expression of Arabidopsis cytosolic ascorbate peroxidase gene in response to ozone or sulphur dioxidePlant Mol. Biol.29479489CrossRefPubMedGoogle Scholar
  23. Kocsya, G., Galibaa, G., Brunoldb, C. 2001Role of glutathione in adaptation and signaling during chilling and cold acclimation in plantsPhysiol. Plant113158164Google Scholar
  24. Levitt, J. 1980Responses of Plants to Environmental Stresses: WaterRadiation, Salt and Other Stresses2Academic PressNew York, USA25280Google Scholar
  25. Lopez-Delgado, H., Dat, J.F., Foyer, C.H., Scott, I.M. 1998Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2J. Exp. Bot.49713720CrossRefGoogle Scholar
  26. Mittler, R., Zilinskas, B.A. 1992Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidaseJ. Biol. Chem.2622180221807Google Scholar
  27. Nakano, Y., Asada, K. 1981Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplastsPlant Cell Physiol.22867880Google Scholar
  28. Noctor, G., Arisi, A.C.M., Jouanin, L., Kunert, K.J., Rennenberg, H., Foyer, C.H. 1998Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transgenic plantsJ. Exp. Bot.49623647Google Scholar
  29. O’Kane, D., Gill, V., Boyd, P., Burdon, R. 1996Chilling, oxidative stress and antioxidant response in Arabidopsis thallana callusPlanta198371377PubMedGoogle Scholar
  30. Patterson, B.D., Machae, E.A., Ferguson, I.B. 1984Estimation of hydrogen peroxide in plant extracts using titanium(IV)Anal. Biochem.139487492CrossRefPubMedGoogle Scholar
  31. Palva, T.K., Hurting, M., Saindrenan, P. 1994Salicylic acid induced resistance to Erwinia carotovora subsp. cartovora in tobaccoMol. Plant Microbe Interact.7356363Google Scholar
  32. Rao, M.V., Paliyath, G., Ormrod, P., Murr, D.P., Watkins, C.B. 1997Influence of salicylic acid on H2O2 production, oxidative stress and H2O2-metabolizing enzymesPlant Physiol.115137149CrossRefPubMedGoogle Scholar
  33. Rasmussaen, J.B., Hammerschmidt, R., Zook, M.N. 1991Systemic induction of salicylic acid accumulation in cucumber after inoculation with Pseudomonas syringae pv SyringaePlant Physiol.9713421347Google Scholar
  34. Stewart, R.C., Bewley, J.D. 1980Lipid peroxidation associated with accelerated aging of soybean axesPlant Physiol.65245248Google Scholar
  35. Sharma, Y.K., Leon, J., Raskin, l., Davis, K.R. 1996Ozone induced responses in Arabidopsis thaliana – the role of salicylic acid in the accumulation of defence-related transcripts and induced resistanceProc. Natl. Acad. Sci. USA9350995104PubMedGoogle Scholar
  36. Shirasu, K., Nakajima, H., Rajasekhar, V.K., Dixon, R.A., Lamb, C. 1997Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signals in the activation of defense mechanismsPlant Cell9261270CrossRefPubMedGoogle Scholar
  37. Tasgin, E., Atici, O, Nalbantoglu, B. 2003Effects of salicylic acid and cold on freezing tolerance in winter wheat leavesPlant Growth Regul.41231236Google Scholar
  38. Camp, W., Montagu, M.V., Inze, D. 1998H2O2NO: redox signals in disease resistanceTrends Plant Sci.3330334Google Scholar
  39. Wang, C.Y. 1995Temperature preconditioning affects glutathione content and glutathione reductase activity in chilled zucchini squashJ. Plant Physiol.145148152Google Scholar
  40. Wang, L.J., Huang, W.D., Yu, F.Y. 2001Effect of elevated temperature on transportion and distribution of 14C-salicylic acid in grape seedlingsActa Phytophys. Sinica27129134Google Scholar
  41. Yalpani, N., Enyedi, A.J., Leon, J., Raskin, I. 1994Ultraviolet light and ozone stimulate accumulation of salicylic acidpathogenesis-related proteins and virus resistance in tobaccoPlanta193372376CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Institute of BotanyChinese Academy of SciencesBeijingChina

Personalised recommendations