Advertisement

Plant Growth Regulation

, Volume 45, Issue 3, pp 251–257 | Cite as

Ectopic expression of MAP kinase inhibits germination and seedling growth in transgenic rice

  • Da-Eun Lee
  • Kyoungwhan Back
Article

Abstract

Jasmonic acid (JA) inhibits seed germination and post-germination growth. We previously generated transgenic rice plants that overexpress wound-inducible MAP kinase from pepper. The transgenic rice plants accumulated about three times more JA than did the wild-type. The transgenic rice seeds required two more days to germinate, as compared to the wild-type, and shoot and root growth was inhibited. The root and shoot length of transgenic plants were comparable to those of the wild-type treated with 10 μM JA. In addition, the transcription level of storage proteins, such as prolamin and glutelin, decrseased more slowly in transgenic lines than in the wild-type during germination. MAP kinase activity was clearly enhanced in the embryos of transgenic rice plants during germination. The inhibition of the germination and growth of transgenic rice plants was attributed to the overexpression of the MAP kinase transgene, which resulted in a subsequent increase in both MAP kinase activity and JA synthesis.

Keywords

Jasmonic acid MAP kinase Seed germination Transgenic rice 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ellis, C., Turner, J.G. 2002A conditionally fertile coi1 allele indicates cross-talk between plant hormone signaling pathways in Arabidopsis thaliana seeds and young seedlingsPlanta215549556CrossRefPubMedGoogle Scholar
  2. 2.
    Farmer, E.E., Ryan, C.A. 1990Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leavesProc. Natl. Acad. Sci. USA8777137716PubMedGoogle Scholar
  3. 3.
    Feys, B.J.F., Benedetti, C.E., Penfold, C.N., Turner, J.G. 1994Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterileinsensitive to methyl jasmonateand resistant to a bacterial pathogenPlant Cell6751759CrossRefPubMedGoogle Scholar
  4. 4.
    Lee, D.E., Lee, I.J., Han, O., Baik, M.G., Han, S.S., Back, K. 2004Pathogen reistanace of transgenic rice plants mitogen-activated protein kinase 1, MK1, from Capsicum annuumMol. Cells178185PubMedGoogle Scholar
  5. 5.
    Sembdner, G., Parthier, B. 1993The biochemistry and the physiological and molecular action of jasmonatesAnnu. Rev. Plant Physiol. Plant Mol. Biol.33569589CrossRefGoogle Scholar
  6. 6.
    Seo, S., Sano, H., Ohashi, Y. 1999Jasmonate-based wound signal transduction requires activation of WIPK, a tobacco mitogen-activated protein kinasePlant Cell11289298CrossRefPubMedGoogle Scholar
  7. 7.
    Shewry, P.R., Harford, N.G. 2002Cereal seed storage proteins: structures, properties and role in grain utilizationJ. Exp. Bot.53947958CrossRefPubMedGoogle Scholar
  8. 8.
    Shin, H.J., Lee, D.E., Shin, D.H., Kim, K.U., Kim, H.Y., Ohashi, Y., Han, O., Baik, M.K., Back, K. 2001Molecular cloning and cultivar expression of MAP kinases from Capsicum annuumMol. Cells114854PubMedGoogle Scholar
  9. 9.
    Staswick, P.E. 1992Jasmonategenes, and fragrant signalsPlant Physiol.99804807Google Scholar
  10. 10.
    Staswick, P.E., Huang, J., Rhee, Y. 1991Nitrogen and methyl jasmonate induction of soybean vegetative storage protein genesPlant Physiol.96130136Google Scholar
  11. 11.
    Staswick, P.E., Su, W., Howell, S.H. 1992Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutantProc. Natl. Acad. Sci.8968376840PubMedGoogle Scholar
  12. 12.
    Testerink, C., Vennik, M., Kijne, J.W., Wang, M., Heimovaara-Dijkstra, S. 2000Inactivation of a MAPK like protein kinase and activation of a MBP kinase in germinating barley embryosFEBS Lett.4845559CrossRefPubMedGoogle Scholar
  13. 13.
    Wilen, R.W., van Rooijen, G.J.H., Pearle, D.W., Pharis, R.P. Holbrook L.A., Moloney, M.M. 1991Effects of jasmonic acid on embryo-specific processes in BrassicaLinum oilseedsPlant Physiol.95399405Google Scholar
  14. 14.
    Yano, H., Wong, J.H., Cho, M.-J., Buchanan, B.B. 2001Redox changes accompanying the degradation of seed storage proteins in germination ricePlant Cell Physiol.42879883CrossRefPubMedGoogle Scholar
  15. 15.
    Yildiz, K., Yilmaz, H. 2002Effect of jasmonic acidACC and ethephon on pollen germination in strawberryPlant Growth Regul.38145148CrossRefGoogle Scholar
  16. 16.
    Zhang, S., Liu, Y., Klessig, D.F. 2000Multiple levels of tobacco WIPK activation during the induction of cell death by fungal elicitinsPlant J.23339347CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Biotechnology, Agricultural Plant Stress Research Center, Biotechnology Research InstituteChonnam National UniversityGwangjuKorea
  2. 2.Department of Biological Science and Biotechnology, College of Agriculture and Life Sciences Chonnam National UniversityGwangjuKorea

Personalised recommendations