Advertisement

Plant Growth Regulation

, Volume 44, Issue 3, pp 199–206 | Cite as

The effect of reactive oxygen species on ethylene production induced by osmotic stress in etiolated mungbean seedling

  • Desen KeEmail author
  • Guchou Sun
Article

Abstract

After 10 h osmotic stress in 25% polyethylene glycol (PEG6000) solution (−1.8 MPa) at 25 °C in darkness, the etiolated mungbean seedlings were transferred to pure water for recovery. The ethylene release rate and the level of reactive oxygen species (ROS), including superoxide radical (O2) and hydrogen peroxide (H2O2), were investigated during the recovery process. The results showed that ethylene production rate and amount of ROS increased dramatically after osmotic stress, and a close correlation was observed between ethylene release rate and concentrations of ROS. Inhibitors of ethylene biosynthesis, aminoethoxyvinylglycine (AVG) or aminooxyacetic acid (AOA), could reduce the ethylene release rate, but had no significant influence to the content of O2 and H2O2. As well as, silver thiosulfate (STS), an inhibitor of ethylene action, exhibited no obvious effect to the concentration of ROS, showing stress-inducible ethylene was not the cause for the increase of stress-inducible ROS. On the other hand, exogenous generator of superoxide radical (methylviologen, MV, or sodium dithionite, Na2S2O4) could enhance the ethylene production evidently, which could be inhibited by exogenous scavenger of superoxide radical (superoxide dismutase, SOD, or 1, 4-diazabicyclo (2,2,2) octane, DABCO). However, either exogenous H2O2 or catalase (CAT) had no significant influence on ethylene production. The results suggested that it was superoxide radical but not H2O2which was involved directly in osmotic stress-inducible ethylene biosynthesis. The dual-role of superoxide radical on stress ethylene biosynthesis was also discussed.

Keywords

Ethylene Mungbean seedling Osmotic stress Reactive oxygen species 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Banga, M., Slaa, E.J., Blom, C.W.P.M., Voesenek, L.A.C.J. 1996Ethylene biosynthesis and accumulation under drained and submerged conditionsPlant Physiol.112229237Google Scholar
  2. Greenwald, R.A. 1987Handbook of Methods for Oxygen Radical Research.CRC pressBoca Raton, Florida5570Third PrintingGoogle Scholar
  3. Grichko, V.P., Glick, B.R. 2001Ethylene and flooding stress in plantsPlant Physiol. Biochem.3919Google Scholar
  4. Hagar, H., Ueda, N., Shah, S.V. 1996Role of reactive oxygen metabolites in DNA damage and cell death in chemical hupoxic injury to LLC-PK1 cellsAm. J. Physiol.271209215Google Scholar
  5. Jones, M.L., Woodson, W.R. 1999Differential expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in carnationPlant Physiol.119755764Google Scholar
  6. Ke, D.S., Wang, A.G., Sun, G.C. 2002The effect of active oxygen on the activity of ACC synthase induced by exogenous IAAActa Botanica Sinica44551556Google Scholar
  7. Ke, D.S., Wang, A.G., Sun, G.C. 2003The role of active oxygen in chilling-induced ethylene production in etiolated mungbean seedlingsActa Phytophysiol. Sinica29127132Google Scholar
  8. Llop-Tous, I., Barry, C.S., Grierson, D. 2000Regulation of ethylene biosynthesis in response to pollination in tomato flowersPlant Physiol.123971978Google Scholar
  9. Low, P.S., Merida, J.R. 1996The oxidative burst in plant defense: function and signal transductionPhysiol. Plant96533542Google Scholar
  10. McRac, D.G., Baker, J.E. 1982Evidence for involvement of the superoxide radical in the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by pea microsomal membranesPlant Cell Physiol.23375383Google Scholar
  11. Nakajima, N., Matsuyama, T., Masanori Tamaoki, M., Saji, H., Aono, M., Kubo, A., Kondo, N. 2001Effects of ozone exposure on the gene expression of ethylene biosynthetic enzymes in tomato leavesPlant Physiol. Biochem.39993998Google Scholar
  12. Nandwal, A.S., Maan, A., Kundu, B.S., Sheokand, S., Kamboj, D.V., Sheoran, A., Kumar, B., Dutta, D. 2000Ethylene evolution and antioxidant defense mechanism in Cicer arietinum roots in the presence of nitrate and aminoethoxyvinylglycinePlant Physiol. Biochem.38709715Google Scholar
  13. Nara, A., Takeuchi, Y. 2002Ethylene evolution from tobacco leaves irradiated with UV-BJ. Plant Res.115247253Google Scholar
  14. Patterson, B.D., Mackae, E.A., Ferguson, J.B. 1984Estimation of hydrogen peroxide in plant extracts using titanium (IV)Ann. Biochem.139487492Google Scholar
  15. Penninckx, I.A.M.A., Thomma, B.P.H.J., Buchala, A., Metraux, J.P., Broekaert, W.F. 1998Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in ArabidopsisPlant Cell1021032113CrossRefPubMedGoogle Scholar
  16. Wang, A.G., Luo, G.H. 1990Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plantsPlant Physiol. Commun.65557Google Scholar
  17. Wasternack, C., Parthier, B. 1997Jasmonate signalled plant gene expressionTrends Plant Sci.2302307Google Scholar
  18. Watanabe, T., Sakai, S. 1998Effects of active oxygen species and methyl jasmonate on expression of the gene for a wound-inducible 1-aminocyclopropane-1-carboxylate synthase in winter squash (Cucurbita maxima)Planta206570576Google Scholar
  19. Watanabe, T., Seo, S., Sakai, S. 2001Wound-induced expression of a gene for 1-aminocyclopropane-1-carboxylate synthase and ethylene production are regulated by both reactive oxygen species and jasmonic acid in Cucurbita maxima. Plant PhysiolBiochem.39121127Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.South China Institute of BotanyThe Chinese Academy of SciencesGuangzhou ReYiJuThe People’s Republic of China
  2. 2.Department of BiologyGuangzhou UniversityGuangzhou GuiHuaGangThe People’s Republic of China

Personalised recommendations