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Plant Growth Regulation

, 44:243 | Cite as

The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida

  • Xiangchun Meng
  • Tim Xing
  • Xiaojing Wang
Article

Abstract

In the present work, the pigmentation regulated by light was investigated in ray floret (rf) of Gerbera hybrida. When inflorescences from stage 1 were covered with aluminium foil in vivo the pigmentation of the rf petals was strongly blocked and the gene expression of CHS (Chalcone synthase) and DFR (Dihydroflavonol-4-reductase) was inhibited. Similar results were obtained when the detached rfs were cultured in vitro. Covering of the leaves on the plants resulted in reduced pigmentation compared with the covering of inflorescences in vivo. Removal of the green bracts did not affect the pigmentation significantly and the anthocyanin concentration was maintained at a level similar to that of the control. The ultrastructure of the plastids in rf petals was examined to investigate the possible role of photosynthesis in light regulation of flower pigmentation. Plastids within rf epidermal cells showed a characteristic chloroplast morphology in flowers at stage 2, which deteriorated by stage 3. They then changed to a chromoplast-like structure in fully opened rf petals (stage 6). Similar chromoplast-like structures were observed in the plastids of the rf petals from inflorescences both shaded in vivo and in vitro. Additionally, DCMU, a photosynthetic inhibitor, did not show a significant effect on light-induced anthocyanin accumulation. Our data suggest that light is an important factor for pigmentation of rf petal in Gerbera and the petal itself acts as a light sensor site to perceive the light signal. From the different light qualities evaluated, blue light promoted gene expression of CHS and DFR, and red light enhanced the gene expression of CHS, indicating the photoreceptors responding to blue and red light involved in the photoregulation of flower pigmentation in Gerbera.

Keywords

Anthocyanin CHS and DFR gene expression Light Photoreceptor Ray floret (rf) 

Abbreviations

CHS

chalcone synthase

DCMU

dichlorophenyldimethyl urea

DFR

dihydroflavonol-4-reductase

rf

ray floret

References

  1. 1.
    Biran, I., Halevy, A.H. 1974Effects of varying light intensity and temperature treatments applied to whole plants, or locally to leaves or flower buds, on growth and pigmentation of ‘Baccara’ RosesPhysiol. Plant31175179Google Scholar
  2. 2.
    Brett, D.W., Sommekard, A.P. 1986Ultrastructural development of plastids in the epidermis and starch layers of glossy Ranunculus petalsAnn. Bot.58903910Google Scholar
  3. 3.
    Dong, Y.H., Beuning, L., Davies, K., Mitra, D., Morris, B., Kootstra, A. 1998Expression of pigmentation genes and photo-regulation of anthocyanin biosynthesis in developing Royal Gala apple flowersAustr. J. Plant Physiol.25245252CrossRefGoogle Scholar
  4. 4.
    Endt, D.V., Kijne, J.W., Memelink, J. 2002Transcription factors controlling plant secondary metabolism: what regulates the regulators?Phytochemistry61107114CrossRefPubMedGoogle Scholar
  5. 5.
    Griesbach, R.J. 1992Correlation of pH and light intensity on flower color in potted Eustoma grandiflorum GriseHortScience27817818Google Scholar
  6. 6.
    Hartmann, U., Valentine, W.J., Christie, J.M., Hays, J., Jenkins, G. I., Weisshaar, B. 1998Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression systemPlant Mol. Biol.36741754PubMedGoogle Scholar
  7. 7.
    Helariutta, Y., Elomaa, P., Kotilainen, M., Griesbach, R.J., Schroder, J., Teeri, T.H. 1995Chalcone synthase-like genes active during corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida (Asteraceae)Plant Mol. Biol.284760PubMedGoogle Scholar
  8. 8.
    Holton, T.A., Cornish, E.C. 1995Genetics and biochemistry of anthocyanin biosynthesisPlant Cell710171083Google Scholar
  9. 9.
    Jackson, J.A., Jenkins, G.I. 1995Extension-growth responses and expression of flavonoid biosynthesis genes in the Arabidopsis hy4 mutantPlanta197233239PubMedGoogle Scholar
  10. 10.
    Katz, A., Weiss, D. 1998Photocontrol of chs gene expression in petunia flowersPhysiol. Plant102210216Google Scholar
  11. 11.
    Kubasek, W.L., Ausubel, F.M., Shirley, B.W. 1998A light-independent developmental mechanism potentiates flavonoid gene expression in Arabidopsis seedlingsPlant Mol. Biol.37217223PubMedGoogle Scholar
  12. 12.
    Levy, Y.Y., Dean, C. 1998The transition to floweringPlant Cell1019731989PubMedGoogle Scholar
  13. 13.
    Martin, C., Prescott, A., Mackay, S., Bartlett, J., Vrijlandt, E. 1991Control of anthocyanin biosynthesis in flowers of Antirrhium majus Plant J.13749PubMedGoogle Scholar
  14. 14.
    Martin, C., Gerats, T. 1993Control of pigment biosynthesis genes during petal developmentPlant Cell512531264PubMedGoogle Scholar
  15. 15.
    Meng, X., Wang, X. 2004Regulation of flower development and anthocyanin accumulation in Gerbera hybrida J. Hort. Sci. Biotech.79131137Google Scholar
  16. 16.
    Moscovici, S., Moalem-Beno, D., Weiss, D. 1996Leaf-mediated light responses in petunia flowersPlant Physiol.11012751282PubMedGoogle Scholar
  17. 17.
    J. Sambrook, D.W. Russel. 2001. Molecular cloning: a laboratory manual. Cold Sping Harbor Laboratory Press,pp.518--552Google Scholar
  18. 18.
    Splet, C., Quattrocchio, F., Mol, J., Koes, R. 2002ANTHOCYANIN 1 of petunia controls pigment synthesis, vacular pH, and seed coat development by genetically distinct mechanismPlant Cell1421212135Google Scholar
  19. 19.
    Weiss, D. 2000Regulation of flower pigmentation and growth: multiple signaling pathways control anthocyanin synthesis in expanding petalsPhysiol. Plant110152157Google Scholar
  20. 20.
    Weiss, D., Halevy, A.H. 1991The role of light reactions in the regulation of anthocyanin synthesis in petunia corollasPhysiol. Plant81127133Google Scholar
  21. 21.
    Winkel-Shirley, B. 2001It takes a garden. how work on diverse plant species has contributed to an understanding of flavonoid metabolismPlant Physiol.12713991404PubMedGoogle Scholar
  22. 22.
    Yu, D., Kotilainen, M., Pöllänen, E., Mehto, M., Elomaa, P., Helariutta, Y., Albert, V.A., Teeri, T.H. 1999Organ identity genes and modified patterns of flowers development in Gerbera hybrida (Asteraceae)Plant J.175162PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.College of Life SciencesSouth China Normal University, Guangdong Key Lab of Biotechnology for Plant DevelopmentGuangzhouP.R. China
  2. 2.Department of BiologyCarleton UniversityOttawaCanada
  3. 3.Fruit Tree Research Institute ScienceGuangdong Academy of Agricultural SciencesGuangzhouP.R. China

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