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Realizing hybrids between the cultivated peanut (Arachis hypogaea L.) and its distantly related wild species using in situ embryo rescue technique

  • Chuan Tang WangEmail author
  • Xiu Zhen Wang
  • Zhi Wei Wang
  • Qiu Mei Yu
  • Yue Yi Tang
  • Qi Wu
  • Shu Tao Yu
Short Communication
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Abstract

Cross-incompatibility between peanut cultigen and species outside section Arachis, impedes the transfer of desirable traits such as high resistance, good quality and high productivity from incompatible species in cultivar development. To recover hybrids from 4 incompatible crosses involving 3 wild species, viz., A. pusilla Benth., A. appresipilla Krapov. & W.C. Greg. and A. paraguariensis Chodat & Hassl., an in situ embryo rescue technique was exploited, resulting in hybrid seeds from all the 4 crosses. The key of this technique is to apply growth substances to pollinated flower bases, and the next step is to wait and harvest seeds directly, whose hybridity is to be tested by miniature inverted-repeat transposable element markers. The technique is advantageous over its in vitro counterpart in earlier intervention, easier operation, and shorter duration required for obtaining hybrids. These superiorities warrant its wide use in the genus Arachis as well as in other plant taxa where post-zygotic hybridization barriers also exist.

Keywords

Peanut Wild species Cross-incompatibility Hybridization barrier In situ embryo rescue 

Notes

Funding

This study was funded by Ministry of Agriculture, People’s Republic of China (CARS-13), Xinjiang Production and Construction Corps, People’s Republic of China (2018BC012), Yantai Science and Technology Bureau, People’s Republic of China (2018ZDCX), and Taishan Industry Leading Talents Special Fund (LJNY201808).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Chuan Tang Wang
    • 1
    • 2
    Email author
  • Xiu Zhen Wang
    • 1
  • Zhi Wei Wang
    • 1
  • Qiu Mei Yu
    • 1
  • Yue Yi Tang
    • 1
  • Qi Wu
    • 1
  • Shu Tao Yu
    • 1
  1. 1.Shandong Peanut Research Institute (SPRI)QingdaoChina
  2. 2.Luhua Group CompanyLaiyangChina

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