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Investigation of the genetic variation of anise (Pimpinella anisum L.) using RAPD and ISSR markers

  • R. Refika Akçali GiachinoEmail author
Research Article
  • 23 Downloads

Abstract

Anise (Pimpinella anisum L.) is an annual spice and medicinal plant belonging to the family Apiaceae. Only a limited number of anise breeding studies are available. Therefore, genetic variation among anise landraces has not been extensively examined with molecular markers. In this study, the molecular diversity of 15 Turkish anise landraces collected from different regions of Turkey and four foreign landraces obtained from Cyprus, Syria and France were analyzed using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. The discriminatory power of these markers was assessed by calculating various marker parameters, namely percent polymorphism, polymorphism information content (PIC), resolving power (RP), and marker index (MI). The genomic DNA of 19 anise landraces was amplified with nine RAPD primers that generated 71 polymorphic bands and five ISSRs primers that produced 45 polymorphic bands. The percentage of polymorphic loci was 59.8% in RAPD profiling and 51.5% in ISSR profiling. The average PIC values obtained using RAPD and ISSR markers were 0.40 and 0.38, respectively. The mean RP (2.90 and 2.67) and MI values (1.23 and 1.05) of the RAPD assay were found to be somewhat higher than those of the ISSR assay, but ISSR was more favorable than the RAPD techniques in terms of presenting the phylogenetic relationships in anise landraces. A UPGMA cluster grouped the 19 anise landraces into two major clusters based on both markers. The RAPD and ISSR marker systems were found to be useful for determining genetic diversity in P. anisum L. and identifying variation among landraces.

Keywords

Anise Pimpinella anisum L. RAPD ISSR markers Molecular characterization Fingerprinting 

Notes

Acknowledgements

I thank the Commission of Scientific Research Projects of Ege University for financial support to carry out this study (Project No: 2013/OMYO/001). I am grateful to Dr. Tansel Kaygısız Aşçıoğul and Dr. Dilşat Yeğenoğlu for their assistance in statistical calculations of the data presented in this manuscript. I also thank Elif Denizaslanı and Christine Taylor for their suggestions to improve the language of the text. Finally, I would like to thank the reviewers for providing useful comments on the previous draft of this article.

Compliance with ethical standards

Conflict of interest

The author declares that they have no conflict of interest.

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Field Crops, Faculty of AgricultureEge UniversityIzmirTurkey

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