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The root transcriptome analyses of peanut wild species Arachis correntina (Burkart) Krapov. & W.C. Gregory and cultivated variety Xiaobaisha in response to benzoic acid and p-cumaric acid stress

  • Zhong LiEmail author
  • Faqian Xiong
  • Wenfeng Guo
  • Changming Mo
  • Haining Wu
  • Lin Du
Research Article
  • 9 Downloads

Abstract

Allelopathy is the main cause of continuous cropping obstacles. Peanut wild species Arachis correntina (Burkart) Krapov. & W.C. Gregory is more resistant to continuous cropping obstacle than cultivated peanut, but its molecular mechanism in response to allelochemicals remains unknown. Benzoic acid (BA) and p-cumaric acid (PCA) are known allelochemical. To gain more insight into cellular response to BA and PCA, we applied high-throughput genetic sequencing to study the transcriptome changes of peanut cultivated variety Xiaobaisha and wild species A. correntina in the presence of 2 mM BA and PCA. The result showed that A. correntina resistance to BA and PCA stress was more weaker than Xiaobaisha. BA and PCA decreased significant shoot length and dry matter weight of A. correntina. Differentially expressed genes (DGEs) related to flavonoid biosynthesis, phenylpropanoid biosynthesis, plant–pathogen interaction and plant hormone signal transduction were transcribed less in A. correntina than Xiaobaisha under BA and PCA stress, including the less up-regulated genes involving in phenylpropane and flavonoid biosynthesis, detoxifying enzymes, Auxin responsive protein, WRKY family and Ef-hand, which might contribute to A. correntina’s weak resistance to BA and PCA stress. The results showed that the resistance to single allelopathic substance might not be the reason for A. correntina resistance to continuous cropping obstacle. In addition, DGEs of both A. correntina and Xiaobaisha were significantly enriched in the pathways associated with isoflavonoid biosynthesis and glutathione metabolism under BA and PCA stress. This was the first report on identification of DGEs under BA and PCA stress between peanut cultivated variety and wild species. The result would be great helpful to insight into the mechanisms of peanut response to allelochemicals stress.

Keywords

Benzoic acid p-cumaric acid Peanut Transcriptome Differentially expressed genes Function analysis 

Notes

Acknowledgements

This study was funded by the Science Development Foundation of Guangxi Academy of Agricultural Sciences (Nos. 2018JZ35, 2017JZ15), and the National Natural Science Foundation of China (Nos. 31460115, 31660087, 31660428).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animals rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

10722_2019_859_MOESM1_ESM.docx (130 kb)
Supplementary material 1 (DOCX 129 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Zhong Li
    • 1
    Email author
  • Faqian Xiong
    • 2
  • Wenfeng Guo
    • 1
  • Changming Mo
    • 1
  • Haining Wu
    • 2
  • Lin Du
    • 3
  1. 1.Guangxi Crop Genetic Improvement and Biotechnology LabGuangxi Academy of Agricultural SciencesNanningChina
  2. 2.Guangxi Academy of Agricultural Sciences Economic Crops Research InstituteNanningChina
  3. 3.Guangxi Science and Technology MuseumNanningChina

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