A new male sterile line of “Duanye-13” radish (Raphanus sativus L.) produced by ethyl methanesulfonate mutagenesis

  • Shisheng LiEmail author
  • Nana Zhang
  • Jingcai Li
  • Jun Xiang
Short Communication


Mutations in radish can be induced by exposure to ethyl methanesulfonate (EMS), and this method is suitable for obtaining target traits in new radish materials. We investigated the optimal concentration of EMS for mutagenesis on the radish variety Duan-Ye No. 13 (DY13). Treating DY13 radish seeds with 0.5% EMS led to the development of sterile mutants. During 3 years of continuous hybridization and tracing, male fertility remained stable at 100% sterility. Morphological observations showed that the anthers of these male sterile were wrinkled, curled, and browned; disintegration of the wall structure at the pollen tetrad stage caused eventual pollen abortion. Detection of the molecular marker ORF138-F/R confirmed that the target band could be amplified in this radish line; however, the band was not amplified in the corresponding maintainer line. Together, these results show that male sterile lines were successfully selected from DY13 radishes, demonstrating a new and effective method for breeding male sterile radish lines.


Mutagenesis Male sterile Molecular marker Breeding Radish 



Cytoplasmic male sterility


Deoxyribonucleic acid


Duan-Ye No. 13


Charge coupled device




Polymerase chain reaction


Statistical product and service solutions



This work was funded by the National Natural Science Fund of China (31701466) and Hubei Special Project for Development of Science and Technology in Local by Central Guidance (2018ZYYD019).

Compliance with ethical standards

Conflicts of interest

The authors declare no conflicts of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories, Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, College of Biology and Agricultural ResourceHuanggang Normal UniversityHuanggangChina
  2. 2.School of Life SciencesHubei UniversityWuhanChina

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