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Development of anthracnose resistant hybrids of the Greater Yam (Dioscorea alata L.) and interspecific hybrids with D. nummularia Lam.

  • Vincent LebotEmail author
  • Kuttolamadathil Abraham
  • Juliane Kaoh
  • Charles Rogers
  • Tari Molisalé
Research Article
  • 1 Downloads

Abstract

Controlled pollinations were conducted to develop Dioscorea alata L. hybrids resistant to anthracnose (Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. Overall, 10,410 hand pollinations were carried out with Indian (IN) and Vanuatu (VU) clones in 53 different parental combinations, including diploids (2x) and tetraploids (4x) female and male plants. Pollination efficiency and seed set were comparable for IN2x × VU2x (54.6% and 23.0%) and IN2x × IN2x (57.4% and 28.9%). However, seedling survival with better resistance to anthracnose in nursery under natural disease pressure was higher for IN2x × IN2x (67.3%) compared to IN2x × VU2x (47.8%). IN2x female clones were crossed with VU2x and VU4x male clones in 12 parental combinations each. Pollination efficiency was higher for 2x parents (54.6%) compared to 4x parents (47.0%) but seed set (32.8%) and seedling survival (71.7%) were significantly higher for crosses between IN2x × VU4x. Overall, remarkable variation in pollination efficiency, seed set and seedling survival was observed between different parental combinations even when the same pollen parent was used. Interspecific hybridization between D. alata IN2x females and D. nummularia Lam. Vanuatu polyploid male was found to be successful with considerable variation in pollination efficiency, seed set and seedling survival (49.0, 20.8 and 35.3%). The hybrids exhibited intermediate characters between the two species with high tuber yield, anthracnose resistance but with fast tuber flesh oxidation. These results demonstrate the scope of polyploid and interspecific hybridization and the need for the international exchange of germplasm for D. alata genetic improvement.

Keyword

Colletotrichum gloeosporioides Controlled pollination Fruit set Polyploidy Seed set Seedling survival 

Notes

Acknowledgements

This work was financially supported by the ‘Agropolis Fondation’ under the reference ID 1403-023 through the ‘Investissements d’Avenir’ programme (Labex Agro:ANR-10-LABX-0001-01). The World Bank offered to Dr. K. Abraham two short term consultancies for providing technical assistance to the VARTC for the genetic improvement of yam by hybridization for the project ‘Increasing Resilience to Climatic Change and Natural Hazards (IRCCNH)’. Special thanks are due to Mr. Ruffino Pineda, coordinator of the project in VARTC for facilitating the field work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10722_2019_756_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 28 kb)

References

  1. Abang MM, Winter S, Green KR, Hofftmann P, Mignouna HD, Wolf GA (2002) Molecular identification of Collectotrichum gloeosporioides causing yam anthracnose in Nigeria. Plant Pathol 51:63–71CrossRefGoogle Scholar
  2. Abraham K, Nair PG (1990a) Floral biology and artificial pollination in Dioscorea alata L. Euphytica 48:45–51Google Scholar
  3. Abraham K, Nair PG (1990b) Vegetative and pseudogamous parthenocarpy in Dioscorea alata. J Root Crops 16:58–60Google Scholar
  4. Abraham K, Nair PG (1991) Polyploidy and sterility in relation to sex in Dioscorea alata L. (Dioscoreaceae). Genetica 83:93–97CrossRefGoogle Scholar
  5. Abraham K, Nair SG, Seekumari MT, Unnikrishnan M (1986) Seed set and seedling variation in Greater Yam (Dioscorea alata L.). Euphytica 35:337–343CrossRefGoogle Scholar
  6. Abraham K, Nemorin A, Lebot V, Arnau G (2013) Meiosis and sexual fertility of autotetraploid clones of Greater Yam Dioscorea alata L. Genet Res Crop Evol 60(3):819–823CrossRefGoogle Scholar
  7. Aduramiga-Modupe AO, Asiedu R, Odobode A, Owolade OF (2012) Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphism (AFLP) markers. Afr J Biotech 11(33):8189–8195Google Scholar
  8. Araki H, Harada T, Yakuwa T (1983) Some characteristics of interspecific hybrids between Dioscorea japonica Thunb. and Dioscorea opposite Thunb. J Jap Soc Hort Sci 52(2):153–158CrossRefGoogle Scholar
  9. Arnau G, Nemorin A, Maledon E, Abraham K (2009) Revision of ploidy status of Dioscorea alata L. (Dioscoreaceae) by cytogenetic and microsatellite segregation analysis. Theor Appl Gen 118:1239–1249CrossRefGoogle Scholar
  10. Arnau G, Abraham K, Sheela MN, Chair H, Sartie A, Asiedu R (2010) Yams. In: Bradshaw JE (ed) Root and tuber crops: handbook of plant breeding, 7th edn. Springer, New York, pp 127–148CrossRefGoogle Scholar
  11. Arnau G, Bhattacharjee R, Sheela MN, Chair H, Malapa R, Lebot V, Abraham K, Perrier X, Petro D, Penet L, Pavis C (2017) Understanding the genetic diversity and population structure of Yam (Dioscorea alata L.) using microsatellite markers. PLoS One 12(3):e0174150.  https://doi.org/10.1371/journal.pone.0174150 CrossRefGoogle Scholar
  12. Asiedu R, Sartie A (2010) Crops that feed the World. Yams. Yams for income and food security. Food Sec 2:305–315CrossRefGoogle Scholar
  13. Bai KV, Jos JS (1978) Polyploidy in the spinosa variety of Dioscorea esculenta (Lour.) Burk. J Root Crops 4:11–14Google Scholar
  14. Bradshaw JE, Bonierbale M (2010) Potatoes. In: Bradshaw JE (ed) Root and tuber crops. Handbook of plant breeding. Springer, New York, pp 1–52CrossRefGoogle Scholar
  15. Chaïr H, Sardos J, Supply A, Mournet P, Malapa R, Lebot V (2016) Plastid phylogenetics of Oceania yams (Dioscorea spp., Dioscoreaceae) reveals natural interspecific hybridization of the Greater Yam (D. alata). Bot J Lin Soc 180(3):319–333CrossRefGoogle Scholar
  16. Coursey DG (1967) Yams. An account of the nature, origins, cultivation and utilization of the useful members of Dioscoreaceae. Longmans, LondonGoogle Scholar
  17. Egesi CN, Pillay M, Asiedu R, Egunjobi JK (2002) Ploidy analysis in water yam, Dioscorea alata L. germplasm. Euphytica 128:225–230CrossRefGoogle Scholar
  18. Essad S (1984) Geographical variation in base chromosome number and polyploidy within the genus Dioscorea, on the basis of chromosome counts in D. transversa Brown, D. pilosiuscula Bert. and D. trifida L. Agronomie 4:611–617CrossRefGoogle Scholar
  19. FAOSTAT (2017) www.fao.org. Accessed 18 Aug 2017
  20. Green KR, Simons SA (1994) ‘Dead Skin’ on yams (Dioscorea alata) caused by Colletotrichum gloeosporioides). Plant Patho 43:1062–1065CrossRefGoogle Scholar
  21. Green KR, Abang MM, Iloba C (2000) A rapid bioassay for screening yam germplasm for response to anthracnose. Trop Sci 40:132–138Google Scholar
  22. Hsu KM, Tsai JL, Chen MY, Ku HM, Liu SC (2013) Molecular phylogeny of Dioscorea (Dioscoreaceae) in East and Souteast Asia. Blumea 58:21–27CrossRefGoogle Scholar
  23. Jos JS, Bai KV, Hrishi N (1977) Meiosis in a triploid intoxicating yam. J Root Crops 3:17–20Google Scholar
  24. Lebot V (2003) Yam: cultivar selection for disease resistance & commercial potential in Pacific Islands. EU INCO-DC: International Cooperation with Developing Countries Contract Number: ERBIC18CT980309. Final Report. CIRAD, Montpellier, France, p 174. https://agritrop.cirad.fr/513977/1/ID513977.pdf. Accessed 29 Aug 2018
  25. Lebot V, Malapa R (2012) Application of near infrared reflectance spectroscopy to the evaluation of yam (Dioscorea alata) germplasm and breeding lines. J Sci Food Agric 93(7):1788–1797CrossRefGoogle Scholar
  26. Lebot V, Malapa R, Abraham K (2017) The Pacific yam (Dioscorea nummularia Lam.), An underexploited tuber crop from Melanesia. Gen Res Crop Evol 64:217–235CrossRefGoogle Scholar
  27. Malapa R, Arnau G, Noyer JL, Lebot V (2005a) Genetic diversity of the Greater Yam (Dioscorea alata L.) and relatedness to D. nummularia Lam. and D. transversa Br. as revealed with AFLP markers. Gen Res Crop Evol 52:919–929CrossRefGoogle Scholar
  28. Malapa R, Noyer JL, Marchand JL, Lebot V (2005b) Genetic relationship between D. alata and D. nummularia as revealed by AFLP. In: Motley TJ, Zerega N, Cross H (eds) Darwin’s Harvest. Columbia University Press, New York, pp 239–265,390Google Scholar
  29. Martin FW, Cabanillas E (1966) The F1 hybrids of some sapogenin-bearing Dioscorea species. Am J Bot 53(4):350–358CrossRefGoogle Scholar
  30. Martin FW, Ortiz S (1963) Chromosome numbers and behavior in some species of Dioscorea. Cytologia 28:96–101CrossRefGoogle Scholar
  31. Obidiegwu J, Rodriguez E, Ene-Obong E, Loureiro J, Muoneke C, Santos C, Kolesnikova-Allen M, Asiedu R (2010) Ploidy levels of Dioscorea alata L. germplasm determined by flow cytometry. Gen Res Crop Evol 57:351–356CrossRefGoogle Scholar
  32. Onwueme IC (1978) The tropical tuber crops: yams, cassava, sweet potato, cocoyams. Wiley, Chichester, p 234pGoogle Scholar
  33. Otoo E (2017) Yam breeding in Ghana. J Agric Sci 9(10):122–136Google Scholar
  34. Prain D, Burkill IH (1938) An account of the genus Dioscorea in the east. The species which twine to the right with addenda to part I and a summary, Royal Botanic Garden, CalcuttaGoogle Scholar
  35. Rao VR, Bammi RK, Randhawa GS (1973) Interspecific hybridisation in the genus Dioscorea. Ann Bot 37:395–401CrossRefGoogle Scholar
  36. Sreeja T (2013) A comparative study of tuber characters, reproductive biology and in vitro response of minor edible yams (Dioscorea species). Ph. D. Thesis. University of Kerala, Trivandrum. India. p 309Google Scholar
  37. VandenBroucke H, Mournet P, Vignes H, Chair H, Malapa R, Duval MF, Lebot V (2015) Somaclonal variants of taro (Colocasia esculenta Schott) and yam (Dioscorea alata L.) are incorporated into farmers’ varietal portfolios in Vanuatu. Gen Res Crop Evol 63(3):495–511CrossRefGoogle Scholar
  38. Viruel J, Segarra-Moragues JG, Raz L, Forest F, Wilkin P, Sanmartin I, Catalan P (2016) Late cretaceous-early eocene origin of yams (Dioscorea, Dioscoreaceae) in the Laurasian Palaearctic and their subsequent Oligocene-Miocene diversification. J Biogeogr 43:750–762CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.CIRAD, BIOS DepartmentUMR AGAPPort-VilaVanuatu
  2. 2.EmeritusCTCRISreekariyam, ThiruvananthapuramIndia
  3. 3.VARTCLuganvilleVanuatu
  4. 4.FSAPort-VilaVanuatu
  5. 5.DARDMinistry of AgricultureLuganvilleVanuatu

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