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Genetic Resources and Crop Evolution

, Volume 65, Issue 6, pp 1637–1647 | Cite as

Molecular cytogenetic characterization, leaf anatomy and ultrastructure of the medicinal plant Potentilla alba L.

  • Tatiana E. Samatadze
  • Svyatoslav A. Zoshchuk
  • Anna S. Khomik
  • Alexandra V. Amosova
  • Natalya Yu. Svistunova
  • Svetlana N. Suslina
  • Firdaus M. Hazieva
  • Olga Yu. Yurkevich
  • Olga V. Muravenko
Research Article
  • 170 Downloads

Abstract

Potentilla alba L. is a valuable medicinal plant widely used in folk and traditional medicine and particularly promising in complex treatment of thyroid pathology. Natural resources of this species are insufficient due to ever-growing use in contemporary medicine. Comprehensive investigations of different P. alba populations are essential for the successful extension of P. alba plantings. Aiming for a better understanding of karyotype structure, chromosome behaviour in meiosis and developing new diagnostic characters, we performed molecular cytogenetic characterization and leaf structure and ultrastructure analyses of two introduced P. alba samples originating from different habitats. Based on chromosome morphology, distribution of 45S/5S rDNA and DAPI-banding patterns, all chromosomes in the karyotypes were identified and the P. alba chromosomal idiogram was constructed. Our findings confirmed P. alba karyotype stability and also revealed several diagnostic characters of this species: the features of cells of upper and lower leaf epidermis, the presence of calcium oxalate druses and three types of leaf indumentum, essential for evaluation of genetic diversity in different populations, validation of raw materials and further selection progress. The meiotic abnormalities were detected probably related to low pollen activity and indicated the advantages of vegetative propagation in the development of a P. alba plantation system.

Keywords

FISH Karyotype Leaf anatomy and ultrastructure Meiosis Potentilla alba L. 

Notes

Funding

This work was supported by the Program of Fundamental Research for State Academies (No. 01201363824) and Russian Foundation of Basic Research (No. 17-29-08-034; 18-016-00167).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests in this work.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Tatiana E. Samatadze
    • 1
    • 2
    • 3
  • Svyatoslav A. Zoshchuk
    • 1
  • Anna S. Khomik
    • 2
  • Alexandra V. Amosova
    • 1
  • Natalya Yu. Svistunova
    • 3
  • Svetlana N. Suslina
    • 2
  • Firdaus M. Hazieva
    • 3
  • Olga Yu. Yurkevich
    • 1
  • Olga V. Muravenko
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussian Federation
  2. 2.People’s Friendship University of Russia (RUDN University)MoscowRussian Federation
  3. 3.All-Russian Institute of Medicinal and Aromatic PlantsFederal Agency for Scientific OrganizationsMoscowRussian Federation

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