Molecular cytogenetic characterization, leaf anatomy and ultrastructure of the medicinal plant Potentilla alba L.
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Potentilla alba L. is a valuable medicinal plant widely used in folk and traditional medicine and particularly promising in complex treatment of thyroid pathology. Natural resources of this species are insufficient due to ever-growing use in contemporary medicine. Comprehensive investigations of different P. alba populations are essential for the successful extension of P. alba plantings. Aiming for a better understanding of karyotype structure, chromosome behaviour in meiosis and developing new diagnostic characters, we performed molecular cytogenetic characterization and leaf structure and ultrastructure analyses of two introduced P. alba samples originating from different habitats. Based on chromosome morphology, distribution of 45S/5S rDNA and DAPI-banding patterns, all chromosomes in the karyotypes were identified and the P. alba chromosomal idiogram was constructed. Our findings confirmed P. alba karyotype stability and also revealed several diagnostic characters of this species: the features of cells of upper and lower leaf epidermis, the presence of calcium oxalate druses and three types of leaf indumentum, essential for evaluation of genetic diversity in different populations, validation of raw materials and further selection progress. The meiotic abnormalities were detected probably related to low pollen activity and indicated the advantages of vegetative propagation in the development of a P. alba plantation system.
KeywordsFISH Karyotype Leaf anatomy and ultrastructure Meiosis Potentilla alba L.
This work was supported by the Program of Fundamental Research for State Academies (No. 01201363824) and Russian Foundation of Basic Research (No. 17-29-08-034; 18-016-00167).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests in this work.
- Amosova AV, Zemtsova LV, Grushetskaya ZE, Samatadze TE, Mozgova GV, Pilyuk YE, Volovik VT, Melnikova NV, Zelenin AV, Lemesh VA, Muravenko OV (2014) Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers. J Genet 93:123–143CrossRefGoogle Scholar
- Amosova AV, Bolsheva NL, Samatadze TE, Twardovska MO, Zoshchuk SA, Andreev IO, Badaeva ED, Kunakh VA, Muravenko OV (2015) Molecular cytogenetic analysis of Deschampsia antarctica Desv. (Poaceae), Maritime Antarctic. PLoS ONE 10(9):e0138878. https://doi.org/10.1371/journal.pone.0138878 CrossRefPubMedPubMedCentralGoogle Scholar
- Amosova AV, Zemtsova LV, Yurkevich OY, Zhidkova EN, Książczyk T, Shostak NG, Muravlev AA, Artemyeva AM, Samatadze TE, Zoshchuk SA, Muravenko OV (2017) Genomic changes in generations of synthetic rapeseed-like allopolyploid grown under selection. Euphytica 9:213–217Google Scholar
- Bogacheva NG, Meshkov AI, Konyaeva EA, Alent’eva OG (2016) Pharmocognostic study of the rhizomes and the roots Potentilla alba L. Prob Biol Med Pharm Chem 1:28–32Google Scholar
- Bolsheva NL, Dyachenko OV, Samatadze TE, Rachinskaya OA, Zakharchenko TV, Shevchuk NS, Amosova AV, Muravenko OV, Zelenin AV (2016) A karyotype of Mesembryanthemum crystallinum (Aizoaceae) studied by chromosome banding, FISH with rDNA probes and immunofluorescence detection of DNA methylation. Plant Biosyst 160:916–922CrossRefGoogle Scholar
- Dolgova AA, Ladygina EY (2003) Manual of practical pharmacognosy. Meditsina, MoscowGoogle Scholar
- Ghaffari SM (2006) Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. Afr J Biotechnol 5:1450–1453Google Scholar
- Grant V (1981) Plant speciation. Columbia University Press, New YorkGoogle Scholar
- Heywood VH (2007) Flowering plants of the world. Mayflower Books. Elsevier, New YorkGoogle Scholar
- Iwatsubo Y, Naruhashi N (1991) Karyomorphological and cytogenetical studies in Potentilla (Rosaceae) I. Karyotypes of nine Japanese species. Cytologia 56:1–10Google Scholar
- Kaminskiĭ AV, Kiseleva IA, Teplaia EV (2013) Clinical application of Potentilla alba for prevention and treatment of thyroid gland pathologies. Likars’ka Sprava 8:99–108Google Scholar
- Kosman VM, Faustova NM, Pozharitskaya ON, Makarov VG (2013) Accumulation of biologically active compounds in underground parts of composition of Potentilla alba L. after various cultivation terms. Russ J Bioorg Chem 2:139–146Google Scholar
- Kovalenko PG, Antonjuk VP, Maliuta SS (2004) Secondary metabolites production from transformed cells of Glycyrrhiza glabra and Potentilla alba as producers of radio-protective compounds. Ukr Bioorg Acta 1–2:13–22Google Scholar
- Kvacheniuk AN, Kvacheniuk EL (2012) The use of phytotherapy for treatment of thyroid diseases. Likars’ka Sprava 3:99–104Google Scholar
- Ma L, Sun X, Kong X, Galvan JV, Li X, Yang S, Yang Y, Yang Y, Hu X (2015) Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau. J Proteom 112:63–82CrossRefGoogle Scholar
- Matkowski A, Świąder K, Ślusarczyk S, Jezierska-Domaradzka A, Oszmiański J (2006) Free radical scavenging activity of extracts obtained from cultivated plants of Potentilla alba L. and Waldsteinia geoides L. Herva Pol 52(4):91–97Google Scholar
- Muravenko OV, Yurkevich OYu, Bolsheva NL, Samatadze TE, Nosova IV, Zelenina DA, Volkov AA, Popov KV, Zelenin AV (2009) Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis. Genetica 135:245–255CrossRefPubMedGoogle Scholar
- Nirmala A, Rao PN (1996) Genetics of chromosome numerical mosaism in higher plants. Nucleus 39:151–175Google Scholar
- Ossipov VI, Polyakov NA, Sidelnikov AN, Hazieva FM (2017) Proanthocyanidins in the roots and rhizomes of Potentilla alba (Rosaceae). Rastitelnye Resursy 53:114–125Google Scholar
- Paule J, Sharbal TF, Dobes C (2011) Apomoctic and sexual lineages of the Potentilla argentea L. group (Rosaceae): cytotype and molecular genetic differentiation. Taxon 60:721–732Google Scholar
- Samatadze TE, Amosova AV, Melnikova NV, Suslina SN, Zagumennikova TN, Zelenin AV, Bykov VA, Muravenko OV (2014) Comparative cytogenetic study of the tetraploid Matricaria chamomilla L. and Matricaria inodora L. Biol Bull 2:123–132Google Scholar
- Sheidai M, Koobaz P, Zehzad B (2003) Meiotic studies of some Avena L. species and populations. Iran J Sci 14:121–131Google Scholar
- Smyk GK, Krivenko VV (1975) White cinquefoil, an effective agent for treating thyroid gland diseases. Farm Zh 2:58–62Google Scholar
- Stebbins GL (1950) Variation and evolution in plants. Columbia University Press, New YorkGoogle Scholar
- Turchaninova LI (2014) Experience of using phytopreparation Alba (root extract of the Potentilla alba) in complex treatment of thyroid pathology in children and adolescents. Likars’ka sprava 3:125–129Google Scholar
- Wolf T (1908) Monographie der Gattung Potentilla L. Biblioth Bot 71:1–714Google Scholar
- Yurkevich OYu, Naumenko-Svetlova AA, Bolsheva NL, Samatadze TE, Rachinskaya OA, Kudryavtseva AV, Zelenina DA, Volkov AA, Zelenin AV, Muravenko OV (2013) Investigation of genome polymorphism and seed coat anatomy of species of section Adenolinum from the genus Linum. Genet Resour Crop Evol 60:661–676CrossRefGoogle Scholar
- Yuzepchuk SV (1941) Genus Potentilla. In: Shishkin BK (ed) Flora SSSR (Flora of the Soviet Union), vol 10. Leningrad, Moscow, pp 78–223Google Scholar