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Genetic Resources and Crop Evolution

, Volume 65, Issue 6, pp 1581–1590 | Cite as

Genetic diversity, genetic structure and migration routes of wild Brassica juncea in China assessed by SSR markers

  • Xiao-Qin Sun
  • Yan-Qiu Qu
  • Mi-Mi Li
  • Xiao-Ling Song
  • Yue-Yu Hang
Research Article
  • 141 Downloads

Abstract

The wild Brassica juncea (L.) Czern. et Coss., systematically belonging to the genus Brassica L. in the Cruciferae family, has become a noxious weed for cropping systems nowadays. Here, simple sequence repeat (SSR) markers were applied to investigate the genetic diversity, genetic structure and migration routes of the wild B. juncea populations in China. The results showed that a total of 90 alleles, with extensive allelic diversity, were observed at the 11 SSR loci of the wild B. juncea. The STRUCTURE analysis indicated that all the 25 wild populations were best described as belonging to two lineages. High Fst value (0.568), together with the partitioning, provided significant evidence for lineage differentiation in wild B. juncea. The high differentiation between the two lineages was, perhaps, due to limited gene flow (Nm = 0.301) of this species. The analysis of molecular variance with distances among individuals corrected for the dominant nature of SSRs showed that most of the variation (59%) occurred within populations, and the remaining 41% variance was attributed to differences among populations. The distribution of diversity across China was significantly geographically dependent. NJ cluster analysis, based on genetic distance, grouped populations geographically, which further corroborated spatial pattern of two lineages. Based on these results, two routes were proposed for the migration of wild B. juncea in China after its origin from northwest China, heading east along the Yellow River or Yangtze River, respectively. We concluded that China, especially the northwest, is one of the primary origins of B. juncea.

Keywords

Wild Brassica juncea SSR Genetic diversity Genetic differentiation 

Notes

Acknowledgements

Brassica juncea varieties were kindly provided by Jinghua Yang in Zhejiang University. This research was financially supported by the Ability Improvement Project of Jiangsu Social Scientific Research Institutions (#BM2015019) granted to X. Q. Sun, the Youth Foundation of Jiangsu Province (BK20160602) and the National Natural Science Foundation of China (X.L.S, 31270579).

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Supplementary material

10722_2018_628_MOESM1_ESM.jpg (2.1 mb)
Fig. S1 Photos of plants of wild B. juncea populations in China. a. JNT; b. ACH; c. ACZ; d. HLY; e. HZK; f. SZA; g. SHZ; h. QHL; i. QXN; j. ZJH; k. HSY; l. HJZ; m. GGY; n. GAS; o. JJP. Acronyms refer to Table 1 (JPEG 2160 kb)
10722_2018_628_MOESM2_ESM.jpg (427 kb)
Fig. S2 Wild B. juncea usually grown alongside the farmland, accompanied by Brassica crops. Wild B. juncea (indicated by white arrowheads) in a. ZJH sampling site and b. JWY sampling site. Acronyms refer to Table 1 (JPEG 427 kb)
10722_2018_628_MOESM3_ESM.tif (233 kb)
Fig. S3 UPGMA tree based on RPS2 sequence of wild B. juncea and its relatives. The RPS2 sequence of Arabidopsis thaliana was used as outgroup (TIFF 232 kb)

References

  1. Chen CL, Zhou Y, Zhou GF, Chen XQ, Fan YH (1992) Discussion on the origin of mustard (Brassica juncea) in China. Southwest China J Agric Sci 3:3–7Google Scholar
  2. Chen S, Wan Z, Nelson MN, Chauhan JS, Redden R, Burton WA, Lin P, Salisbury PA, Fu T, Cowling WA (2013) Evidence from genome-wide simple sequence repeat markers for a polyphyletic origin and secondary centers of genetic diversity of Brassica juncea in China and India. J Hered 104(3):416–427.  https://doi.org/10.1093/jhered/est015 CrossRefPubMedGoogle Scholar
  3. Chen FB, Liu HF, Yao QL, Fang P (2016) Evolution of mustard (Brassica juncea Coss.) subspecies in China: evidence from the chalcone synthase gene. Genet Mol Res.  https://doi.org/10.4238/gmr.15028045 CrossRefPubMedGoogle Scholar
  4. Dan B, Danzeng SB, Meng X, Sun XL, Wang JL (2009) Analysis of wild B. juncea L. rapeseed germplasm resources in Tibet by using SSR marker. J Anhui Agric Sci 37(31):15609–15613Google Scholar
  5. Doyle JJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem bull 19:11–15Google Scholar
  6. Fang P, Chen FB, Yao QL, Yang KC, Zhou GF, Fan YH, Zhang ZR, Shen JJ, Zhang H (2013) Analysis of genetic diversity in the tuber mustard (Brassica juncea var. tumida Tsen et Lee) in the Yangtze river basin of China. Genet Resour Crop Evol 60(1):129–143CrossRefGoogle Scholar
  7. Gladis T, Hammer K (1992) Die Gaterslebener Brassica-KolIektion - Brassica juncea, B. napus, B. nigrn und B. rapa. Feddes Repertoriurn 103(7–8):469–507Google Scholar
  8. Goudet J (2001) FSTAT version 2.9.3. A program to estimate and test gene diversities and fixation indices. http://www2.unil.ch/popgen/softwares/fstat.htm. Accessed 17 June 2011
  9. Hammer K, Gladis T (2014) Notes on infraspecific nomenclature and classifications of cultivated plants in Compositae, Cruciferae, Cucurbitaceae, Gramineae (with a remark on Triticum dicoccon Schrank) and Leguminosae. Genet Resour Crop Evol 61:1455–1467CrossRefGoogle Scholar
  10. Hammer K, Gladis T, Laghetti G, Pignone D (2013) The wild and the grown—remarks on Brassica. Int J Agric Sci 3:453–480Google Scholar
  11. Hamrick JL, Godt MJW, Murawski DA, Loveless MD (1991) Correlations between species and allozyme diversity: implications for conservation biology. In: Falk DA, Holsinger KE (eds) Genetics and conservation of rare plants. Oxford University Press, New YorkGoogle Scholar
  12. Harlan JR (1975) Geographic patterns of variation in some cultivated plants. J Hered 66(4):182–191CrossRefGoogle Scholar
  13. Huangfu CH, Song XL, Qiang S (2009a) ISSR variation within and among wild Brassica juncea populations: implication for herbicide resistance evolution. Genet Resour Crop Evol 56(7):913–924.  https://doi.org/10.1007/s10722-009-9410-x CrossRefGoogle Scholar
  14. Huangfu CH, Song XL, Qiang S (2009b) Morphological disparities in the epidermal and anatomical features of the leaf among wild Brassica juncea populations. Weed Biol Manag 9:234–242CrossRefGoogle Scholar
  15. Jham GN, Moser BR, Shah SN, Holser RA, Dhingra OD, Vaughn SF, Berhow MA, Winkler-Moser JK, Isbell TA, Holloway RK (2009) Wild Brazilian mustard (Brassica juncea L.) seed oil methyl esters as biodiesel fuel. J Am Oil Chem Soc 86(9):917–926.  https://doi.org/10.1007/s11746-009-1431-2 CrossRefGoogle Scholar
  16. Li Y, Sun X, Yan Q, Guo J, Qiang S, Song X, Li M (2013) Isolation and characterization of microsatellite DNA loci for wild Brassica juncea (Brassicaceae). Genet Mol Res 12(4):5392–5395.  https://doi.org/10.4238/2013.November.8.1 CrossRefPubMedGoogle Scholar
  17. Liu PY (1996) Chinese mustard. China Agriculture Press, Beijing, pp 35–41Google Scholar
  18. Mounicou S, Vonderheide AP, Shann JR, Caruso JA (2006) Comparing a selenium accumulator plant (Brassica juncea) to a nonaccumulator plant (Helianthus annuus) to investigate selenium-containing proteins. Anal Bioanal Chem 386(5):1367–1378CrossRefPubMedGoogle Scholar
  19. Nishizawa T, Tamaoki M, Aono M, Kubo A, Saji H, Nakajima N (2010) Rapeseed species and environmental concerns related to loss of seeds of genetically modified oilseed rape in Japan. GM Crops 1(3):143–156.  https://doi.org/10.4161/gmcr.1.3.12761 CrossRefPubMedGoogle Scholar
  20. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295CrossRefGoogle Scholar
  21. Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57Google Scholar
  22. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155(2):945–959PubMedPubMedCentralGoogle Scholar
  23. Qi XH, Yang JH, Yu JQ, Zhang MF (2009) Genetic and heterosis analysis for important agronomic traits of Chinese vegetable mustard (Brassica juncea) in different environments. Genetica 136(1):89–95.  https://doi.org/10.1007/s10709-008-9316-0 CrossRefPubMedGoogle Scholar
  24. Rieger MA, Lamond M, Preston C, Powles SB, Roush RT (2002) Pollen-mediated movement of herbicide resistance between commercial canola fields. Science 296(5577):2386–2388CrossRefPubMedGoogle Scholar
  25. Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236(4803):787–792CrossRefPubMedGoogle Scholar
  26. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  27. Van Puyvelde K, Van Geert A, Triest L (2010) ATETRA, a new software program to analyse tetraploid microsatellite data: comparison with TETRA and TETRASAT. Mol Ecol Resour 10(2):331–334.  https://doi.org/10.1111/j.1755-0998.2009.02748.x CrossRefGoogle Scholar
  28. Vavilov N (1935) Botanical-geographic principles of selection, p 60. Moskva and Leningrad. Russian Translation in US Dept Agr Bur Plant IndusGoogle Scholar
  29. Wang JL, He Y, Luan YF, Dacizhuoga Zhang YQ (2006) A study on origin, evolution and spread of Brassica in China. Chin Agric Sci Bull 22(8):489–494Google Scholar
  30. Wu XM, Chen BY, Lu G, Wang HZ, Xu K, Guizhan G, Song Y (2009) Genetic diversity in oil and vegetable mustard (Brassica juncea) landraces revealed by SRAP markers. Genet Resour Crop Evol 56(7):1011–1022.  https://doi.org/10.1007/s10722-009-9420-8 CrossRefGoogle Scholar
  31. Yao Q-L, Chen F-B, Fang P, Zhou G-F, Fan Y-H, Zhang Z-R (2012) Genetic diversity of Chinese vegetable mustard (Brassica juncea Coss.) landraces based on SSR data. Biochem System Ecol 45:41–48CrossRefGoogle Scholar
  32. Zhou Y, Xue Qun C, Guang Fan Z, Cai Lin C, Yong Hong F (1990) Isoenzyme analysis of cultivated and wild mustard and Brassica ancestral species. Southwest China J Agric Sci 4:42–46Google Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjingChina
  2. 2.Weed Research LaboratoriesNanjing Agricultural UniversityNanjingChina

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