Genetic Resources and Crop Evolution

, Volume 64, Issue 7, pp 1605–1611 | Cite as

Investigation of intergeneric crossability of Vicia faba L. and Thermopsis turcica Kit Tan, Vural et Küçüködük

Research Article
  • 123 Downloads

Abstract

An unilocular ovary is often observed in the Fabaceae family. Thermopsis turcica Kit Tan, Vural et Küçüködük is an endemic, rare plant species in Turkey that is distinguished by its multi-carpellary apocarpous ovaries. No research has been conducted on the crossing of T. turcica with other species to date. Vicia faba L. (fababean) (2n = 12), which is one of the oldest crops used for human and animal consumption, and Thermopsis turcica (2n = 18) were selected to explore the possibility of increasing yield in edible crops by intergeneric crossing. When T. turcica was used as a male parent, pollen germination and pollen tube growth appeared to be normal and globular embryo formation was observed, but hybrid seeds aborted due to post-fertilization barriers. In contrast, in all crosses in which T. turcica was used as a maternal parent, an embryo was not obtained, and viability of the pistil did not exceed 10 days after pollination. Also, in the samples left to free pollination, pistils died in a few days after anthers were removed just before pre-anthesis. Inability to obtain viable seeds by hybridization of Vicia faba and T. turcica should not be considered as a demonstration of complete incompatibility of the species in intergeneric crosses. The present report constitutes the first study on this issue.

Keywords

Embryo Intergeneric hybridization Pollen Thermopsis turcica Vicia faba 

Abbreviations

DAP

Day after pollination

NGBG

Nezahat Gökyiğit Botanical Garden

P. polyanthus

Phaseolus polyanthus

T. turcica

Thermopsis turcica

V. faba

Vicia faba

Supplementary material

10722_2016_459_MOESM1_ESM.tif (4 mb)
Fig. S1Microscopic view of T. turcica ovules (magnification: 2.52 x; scale bar 100 μm) (TIFF 4081 kb)

References

  1. Borisjuk L, Weber H, Panitz R et al (1995) Embryogenesis of Vicia faba L.: histodifferentiation in relation to starch and storage protein synthesis. J Plant Physiol 147:203–218. doi:10.1016/S0176-1617(11)81507-5 CrossRefGoogle Scholar
  2. Cenkci S, Kargioglu M, Dayan S, Konuk M (2008) In vitro propagation of an endangered plant species, Thermopsis turcica (Fabaceae). Biologia (Bratislava) 63:652–657. doi:10.2478/s11756-008-0125-9 CrossRefGoogle Scholar
  3. Cenkci S, Temel M, Kargioğlu M, Dayan S (2009) Propagation of endangered Thermopsis turcica Kit Tan, Vural and Küçüködük using conventional and in vitro techniques. Turk J Biol 33:327–333. doi:10.3906/biy-0811-1 Google Scholar
  4. Clarke HJ, Wilson JG, Kuo I et al (2006) Embryo rescue and plant regeneration in vitro of selfed chickpea (Cicer arietinum L.) and its wild annual relatives. Plant Cell Tissue Organ Cult 85:197–204. doi:10.1007/s11240-005-9071-1 CrossRefGoogle Scholar
  5. Deng Y, Teng N, Chen S, Chen F, Guan Z, Song A, Chang Q (2010) Reproductive barriers in the intergeneric hybridization between Chrysanthemum grandflorum (Ramat.) Kitam. and Ajania przewalskii Poljak. (Asteraceae). Euphytica 174:41–50CrossRefGoogle Scholar
  6. Eti S (1987) Über das Pollenschlauchwachstum und die Entwicklung der Samenanlagen in Beziehung zum Fruchtansatz und zur Fruchtqualiät bei der Mandarinensorte Clementine (Citrus reticulata Blanco). Dissertation, Hohenheim UniversityGoogle Scholar
  7. Fratini R, Ruiz ML, de la Vega M (2004) Intraspecific and intersubspecific crossing in lentil (Lens culinaris Medik.). Can J Plant Sci 84:981–986CrossRefGoogle Scholar
  8. Gaaliche B, Majdoub A, Trad M, Mars M (2013) Assessment of pollen viability, germination, and tube growth in eight Tunisian caprifig (Ficus carica L.) cultivars. ISRN Agron 2013:1–4. doi:10.1155/2013/207434 CrossRefGoogle Scholar
  9. Hiscock S (2011) Sexual plant reproduction. Ann Bot 108:585–587. doi:10.1093/aob/mcr217 CrossRefPubMedPubMedCentralGoogle Scholar
  10. Linnaæus C (1753) Species plantarum. Gener Relat 1:572. doi:10.1017/CBO9781107415324.004 Google Scholar
  11. Linskens HF, Esser KL (1957) Über eine spezifische Anfärbung der Pollenschläuche im Griffel und die Zahl der Kallosepfropfen nach Selbstung und Fremdung. Naturwissenschaften 44:16. doi:10.1007/BF00629340 CrossRefGoogle Scholar
  12. Mallikarjuna N (1999) Ovule and embryo culture to obtain hybrids from interspecific incompatible pollinations in chickpea. Euphytica 110:1–6CrossRefGoogle Scholar
  13. Muehlbauer F, Tullu A (1997) Vicia faba L. https://www.hort.purdue.edu/newcrop/CropFactSheets/fababean.html. Accessed 22 Jun 2015
  14. Naik SV (1993) Interspecific hybridization and characterization of hybrids in genus Cicer L. Dissertation, Indira Gandhi Krishi Vishwa VidyalayaGoogle Scholar
  15. Ozdemir C, Dural H, Ertugrul K et al (2008) Morphology and anatomy of endemic Thermopsis turcica Kit Tan, Vural and Küçüködük. Bangladesh J Bot 37:105–114. doi:10.3329/bjb.v37i2.1714 Google Scholar
  16. Parra R, Sánchez-Lafuente AM (2010) Within-carpel and among-carpel competition during seed development, and selection on carpel number, in the apocarpous perennial herb Helleborus foetidus L. (Ranunculaceae). Plant Syst Evol 290:225–237CrossRefGoogle Scholar
  17. Schmit V, Debouck DG (1991) Observations on the origin of Phaseolus polyanthus Greenman. Econ Bot 45:345–364CrossRefGoogle Scholar
  18. Singh AK, Bharati RC, Manibhushan NC, Pedpati A (2013) An assessment of faba bean (Vicia faba L.) current status and future prospect. Afr J Agric Res 8:6634–6641CrossRefGoogle Scholar
  19. Tan K, Vural M, Küçüködük M (1983) An unusual new Thermopsis from Turkey. Notes RBG Edinb 40:515–518Google Scholar
  20. Tekdal D, Cetiner S (2014) The determination of self-compatibility status of Thermopsis turcica through histological analysis. J Appl Biol Sci 8:64–67Google Scholar
  21. Thorpe TA, Yeung E (2011) Plant Embryo Culture: Methods and Protocols. Humana PressGoogle Scholar
  22. Toussaint A, Geerts P, Clement F, Mergeai G, Baudoin J (2004) Early abortion in reciprocal crosses between Phaseolus vulgaris and Phaseolus polyanthus, and in vitro culture of immature embryos from these species. Belg J Bot 137:47–54Google Scholar
  23. Vural M (2009) Piyan (Thermopsis turcica). Bağbahçe 25:14–16Google Scholar
  24. Wen J, Li J, Walker JC (2012) Overexpression of a serine carboxypeptidase increases carpel number and seed production in Arabidopsis thaliana. Food and Energy Security 1:61–69CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Biological Sciences and Bioengineering Program, Faculty of Engineering and Natural SciencesSabanci UniversityOrhanli-Tuzla, IstanbulTurkey

Personalised recommendations