Genetic Resources and Crop Evolution

, Volume 63, Issue 7, pp 1255–1271 | Cite as

Effects of traditional management for mescal production on the diversity and genetic structure of Agave potatorum (Asparagaceae) in central Mexico

  • Lizeth I. Félix-Valdez
  • Ofelia Vargas-Ponce
  • Dánae Cabrera-Toledo
  • Alejandro Casas
  • Angélica Cibrian-Jaramillo
  • Lino de la Cruz-Larios
Research Article

Abstract

Agave potatorum is a wild endemic species of Mexico. Its stems are used for mescal production, which cancels sexual reproduction. Agaves extraction from forests decreases their reproductive success and demographic performance. We evaluated patterns of genetic diversity and structure of wild populations under and without extraction in order to identify effects of its use and actions required for conserving genetic variation. This study was conducted with seven SSR markers in 12 populations representing the entire distribution area of the species. Standard parameters of genetic diversity, differentiation, structure and genetic similarity of populations were calculated and analyzed. The populations studied showed intermediate to high genetic diversity (He = 0.36–0.64), compared with other Agave species so far studied. The wild category was the most diverse (HT = 0.87), but without significant differences with respect to populations under extraction (HT = 0.72), and two germplasm banks sampled (HT = 0.69). High structure among populations (ΦPR = 0.38) and inbreeding levels (FIS = 0.26, FIT = 0.55) were identified; a 3 % of genetic diversity being distributed among management status where germplasm banks represent a genetic pool with incipient divergence from the other categories. Bayesian analysis indicated two genetic groups. Our study suggests slight effects of management on genetic diversity of A. potatorum, apparently related to alterations of reproductive biology and pollination patterns.

Keywords

Endemic plant Germplasm banks Incipient domestication Plant extraction Population genetics Tehuacán-Cuicatlán Valley 

References

  1. Addison JA, Hart MW (2005) Spawning, copulation and inbreeding coefficients in marine invertebrates. Biol Lett 1:450–453CrossRefPubMedPubMedCentralGoogle Scholar
  2. Aguirre-Dugua X, Eguiarte LE (2013) Genetic diversity, conservation and sustainable use of wild Agave cupreata and Agave potatorum extracted for mezcal production in Mexico. J Arid Environ 90:36–44CrossRefGoogle Scholar
  3. Alvarez-de Zayas A (1989) Distribución geográfica y posible origen de las Agavaceae. Rev Jard Bot Nac Univ Habana Cuba 10:25–35Google Scholar
  4. Barraza-Morales AF, Sánchez-Teyer L, Robert M, Esqueda M, Gardea A (2006) Genetic variability in Agave angustifolia Haw. at the Sonoran Sierra, México, as determined by AFLP markers. Rev Fitotec Mex 29:1–8Google Scholar
  5. Blancas J, Casas A, Rangel-Landa S, Moreno Calles A, Torres I, Pérez-Negrón E, Solís L, Delgado-Lemus A, Parra F, Arellanes Y, Caballero J, Cortés L, Lira R (2010) Plant Management in the Tehuacán-Cuicatlán Valley, Mexico. Econ Bot 64:287–302CrossRefGoogle Scholar
  6. Byers C, Maughan PJ, Clouse J, Stewart JR (2014) Microsatellite primers in Agave utahensis (Asparagaceae), a keystone species in the Mojave Desert and Colorado Plateau. Appl Plant Sci 2:1–3Google Scholar
  7. Casas A, Caballero J, Mapes C, Zarate S (1997) Manejo de la vegetación, domesticación de plantas y origen de la agricultura en Mesoamérica. B Soc Bot Mex 61:31–47Google Scholar
  8. Casas A, Caballero J, Valiente-Banuet A (1999) Use, Management and domestication of columnar cacti in southcentral Mexico: a historical perspective. J Ethnobiol 191:71–75Google Scholar
  9. Casas A, Otero-Arnaiz A, Pérez-Negrón E, Valiente-Banuet A (2007) In situ management and domestication of plants in Mesoamerica. Ann Bot Lond 100:1101–1115CrossRefGoogle Scholar
  10. Colunga-GarcíaMarín P, Zizumbo-Villareal D (2007) Tequila and other Agave spirits from west-central Mexico: current germplasm diversity, conservation and origin. Biodivers Conserv 16:1653–1667CrossRefGoogle Scholar
  11. Colunga-GarcíaMarín P, Coello-Coello J, Eguiarte LE, Piñero D (1999) Isozymatic variation and phylogenetic relationships between henequén (Agave fourcroydes) and its wild ancestor A. angustifolia (Agavaceae). Am J Bot 86:115–123CrossRefPubMedGoogle Scholar
  12. Colunga-GarcíaMarín P, Zizumbo-Villareal D, Torres-Martínez J (2007) Tradiciones en el aprovechamiento de los Agaves mexicanos: una aportación a la protección legal y conservación de su diversidad biológica y cultural. In: Colunga-GarcíaMarín P, Larque Saavedra A, Eguiarte LE, Zizumbo-Villareal D (eds) En lo ancestral hay futuro: del tequila, los mezcales y otros agaves. Centro de investigación Científica de Yucatán A. C., pp 229–248Google Scholar
  13. Dávila P, Arizmendi MC, Valiente-Banuet A, Villaseñor JL, Casas A, Lira R (2002) Biological diversity in the Tehuacán-Cuicatlán Valley, Mexico. Biodivers Conserv 11:421–442CrossRefGoogle Scholar
  14. Delgado-Lemus A, Casas A, Tellez O (2014a) Distribution, abundance and traditional management of Agave potatorum in the Tehuacán Valley, Mexico: bases for sustainable use of non-timber forest products. J Ethnobiol Ethnomed 10:63CrossRefPubMedPubMedCentralGoogle Scholar
  15. Delgado-Lemus A, Torres I, Blancas J, Casas A (2014b) Vulnerability and risk management of Agave species in the Tehuacán Valley, México. J Ethnobiol Ethnomed 10:53CrossRefPubMedPubMedCentralGoogle Scholar
  16. Earl DA, Vonholdt BM (2012) Structure Harvester: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361CrossRefGoogle Scholar
  17. Eguiarte LE, Souza V, Silva-Montellano A (2000) Evolución de la familia Agavaceae: Filogenia, Biología Reproductiva y Genética de poblaciones. Bol Soc Bot Mex 66:131–150Google Scholar
  18. Eguiarte LE, Aguirre-Planter E, Aguirre X et al (2013) From isoenzymes to genomics: populations genetic and conservation of Agave in México. Bot Rev 79:483–506CrossRefGoogle Scholar
  19. Estrella-Ruíz JP (2008) Efecto de la explotación humana en la biología de la polinización de Agave salmiana y Agave potatorum en el Valle de Tehuacán-Cuicatlán. M.Sc. Dissertation, Universidad Nacional Autónoma de México, Distrito Federal, MéxicoGoogle Scholar
  20. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620CrossRefPubMedGoogle Scholar
  21. Excoffier L, Lischer H (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567CrossRefPubMedGoogle Scholar
  22. Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587PubMedPubMedCentralGoogle Scholar
  23. Felsenstein J (1985) Confidence limits on phylogenies; an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  24. Figueredo JC, Nassar JM (2011) Population genetics of Agave cocui: evidence for low genetic diversity at the southern geographic limit of genus Agave. J Hered 102:306–314CrossRefPubMedGoogle Scholar
  25. García S (2014) Diversidad genética y efecto del cambio climático en la distribución de Quercus potosina (Fagaceae) en Sierra fría, Aguascalientes: un enfoque local para su conservación. B.Sc. Dissertation, Universidad de la Sierra de Juárez. Ixtlán de Juárez, Oaxaca. MéxicoGoogle Scholar
  26. García-Mendoza A (2002) Distribution of Agave (Agavaceae) in Mexico. Cactus Succul J 74:177–186Google Scholar
  27. García-Mendoza A (2010) Revisión taxonómica del complejo Agave potatorum Zucc. (Agavaceae): nuevos taxa y neotipificación. Acta Bot Mex 91:71–93Google Scholar
  28. García-Mendoza A, Galván R (1995) Riqueza de las Familias Agavaceae y Nolinaceae en México. Bol Soc Bot Mex 56:7–24Google Scholar
  29. Gentry HS (1982) Agaves of Continental North America. The University of Arizona Press, TucsonGoogle Scholar
  30. Gómez Z. 2007. Diversidad genética de una bromelia de importancia comercial. M.Sc. Dissertation, Instituto politécnico Nacional. Santa Cruz Xoxocotlán, Oaxaca, MéxicoGoogle Scholar
  31. Hamrick JL, Godt MJW (1996) Effects of the history traits on genetic diversity in plants. Philos Trans R Soc B 351:1291–1298CrossRefGoogle Scholar
  32. Hedrick PW (2005) Genetics of populations, 4th edn. Jones and Bartlett Publishers, SudburyGoogle Scholar
  33. Kalinowski ST (2004) Counting alleles with rarefaction: private alleles and hierarchical sampling designs. Conserv Genet 5:539–543CrossRefGoogle Scholar
  34. Kalinowski ST (2005) HP-RARE 1.0: a computer program for performing rarefaction on measures of allelic richness. Mol Ecol Notes 5:187–189CrossRefGoogle Scholar
  35. Lande R (1988) Genetics and demography in biological conservation. Science 241:1455–1460CrossRefPubMedGoogle Scholar
  36. Lindsay D, Edwards C, Jung M, Bailey P, Lance RF (2012) Novel microsatellite loci for Agave parryi and cross-amplification in Agave palmeri (Agavaceae). Am J Bot 99:295–297CrossRefGoogle Scholar
  37. MacNeish RS (1992) The origins of agriculture and settled life, 1st edn. University of Oklahoma Press, NormanGoogle Scholar
  38. Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220PubMedGoogle Scholar
  39. Martínez-Palacios A, Eguiarte LE, Furnier GR (1999) Genetic diversity of endangered endemic Agave victoriae-reginae (Agavaceae) in the Chihuahuan desert. Am J Bot 86:1093–1098CrossRefPubMedGoogle Scholar
  40. Miller MP (2000) Tools for population genetic analysis (TFPGA). Ver 1.3. Northern Arizona University, ArizonaGoogle Scholar
  41. Miller AJ, Schaal BA (2006) Domestication and the distribution of genetic variation in wild and cultivated populations of the Mesoamerican fruit tree Spondias purpurea L. (Anacardiaceae). Mol Ecol 15:1467–1480CrossRefPubMedGoogle Scholar
  42. Molina-Freaner F, Eguiarte LE (2003) The pollination biology of two paniculate agaves (Agavaceae) from Northwestern Mexico: contrasting roles of bats as pollinators. Am J Bot 90:1016–1024CrossRefPubMedGoogle Scholar
  43. Moreno-Calles AI, Casas A (2010) Agroforestry systems: restoration of semiarid zones in the Tehuacán Valley, Central Mexico. Ecol Restor 28:361–368CrossRefGoogle Scholar
  44. Navarro-Quezada A, González-Chauvet R, Molina-Freaner F, Eguiarte LE (2003) Genetic differentiation in the Agave deserti (Agavaceae) complex of the Sonoran desert. Heredity 90:220–227CrossRefPubMedGoogle Scholar
  45. Nei M (1972) Genetic distance between populations. Am Nat 106:238–292CrossRefGoogle Scholar
  46. Nei M (1978) Estimation of average heteozogosity and genetic distance from a small number of individuals. Genetics 89:583–590PubMedPubMedCentralGoogle Scholar
  47. Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155CrossRefPubMedGoogle Scholar
  48. Padilla C, Montoya Y, Carrillo C (2003) Estandarización de una prueba de pcr para la detección de Brucella sp. Rev Peru Med Exp Salud Pública 20(2):102–104Google Scholar
  49. Parker KC, Trapnell DW, Hamrick JL, Hodgson WC, Parker AJ (2010) Inferring ancient Agave cultivation practices from contemporary genetic patterns. Mol Ecol 19:1622–1637CrossRefPubMedGoogle Scholar
  50. Parra F, Pérez-Nasser N, Lira R, Perez-Salicrup D, Casas A (2008) Population genetics and process of domestication of Stenocereus pruinosus (Cactaceae) in the Tehuacan Valley, Mexico. J Arid Environ 72:1997–2010CrossRefGoogle Scholar
  51. Peakall P, Smouse PE (2005) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  52. Pritchard JK, Wen X, Falush D (2000) Documentation for structure software: Version 2.3. University of Chicago, ChicagoGoogle Scholar
  53. Rangel-Landa S, Dávila P, Casas A (2015) Facilitation of Agave potatorum: an ecological approach for assisted population recovery. For Ecol Manag 347:57–74CrossRefGoogle Scholar
  54. Rzedowski J (2006) Vegetación de México.1st digital edn. Comision Nacional para el conocimiento y uso de la Biodiversidad. MéxicoGoogle Scholar
  55. Sánchez-Teyer F, Moreno-Salazar S, Esqueda M, Barraza A, Robert ML (2009) Genetic variability of wild Agave angustifolia populations based on AFLP: a basic study for conservation. J Arid Environ 73:611–616CrossRefGoogle Scholar
  56. Sanguinetti CJ, Días-Neto E, Simpson AJG (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17:915–919Google Scholar
  57. Scheinvar GE (2008) Genética de poblaciones y biología reproductiva en especies mezcaleras. M.Sc. Dissertation, Instituto de Ecología, UNAM, México. D.F., MéxicoGoogle Scholar
  58. Silva-Montellano A, Eguiarte LE (2003a) Geographic patterns in the reproductive ecology of Agave lechuguilla (Agavaceae) in the Chihuahuan desert: genetic variation, differentiation, and inbreeding estimate. Am J Bot 90:700–706CrossRefPubMedGoogle Scholar
  59. Silva-Montellano A, Eguiarte LE (2003b) Geographic patterns in the reproductive ecology of Agave lechuguilla (Agavaceae) in the Chihuahuan desert. I. Floral characteristics, visitors, and fecundity. Am J Bot 90:377–387CrossRefPubMedGoogle Scholar
  60. Sosa V, De-Nova A (2012) Endemic angiosperm lineages in mexico: hotspots for conservation. Acta Bot Mex 100:293–315Google Scholar
  61. Statsoft, Inc (2013) STATISTICA (data analysis software system), version 12. www.statsoft.com
  62. Torres I, Casas A, Delgado-Lemus A, Rangel-Landa S (2013) Aprovechamiento, demografía y establecimiento de Agave potatorum en el Valle de Tehuacán, México: aportes etnobiológicos y ecológicos para su manejo sustentable. Zonas Áridas 15:1–16Google Scholar
  63. Torres I, Casas A, Vega E, Martínez-Ramos M, Delgado-Lemus A (2015) Population dynamics and sustainable management of mescal agaves in central Mexico: Agave potatorum in the Tehuacán-Cuicatlán Valley. Econ Bot. doi:10.1007/s12231-014-9295-2 Google Scholar
  64. Trejo L (2006) Genética de poblaciones de Agave striata Zucc. M.Sc. Dissertation, Universidad Nacional Autónoma de México. México, DFGoogle Scholar
  65. Valenzuela A (2011) A new agenda for blue agave landraces: food, energy and tequila. GCB Bionergy 3:15–24CrossRefGoogle Scholar
  66. Valenzuela-Zapata AG, Regalado-Pinedo A, Mizoguchi M (2008) Influencia asiática en la producción de mezcal en la costa de Jalisco. El caso de la raicilla. Mex Cuenca Pac 11:91–116Google Scholar
  67. Vargas-Ponce O, Zizumbo-Villarreal D, Martínez-Castillo J, Coello-Coello J, Colunga-GarcíaMarín P (2009) Diversity and structure of landraces of Agave grown for spirits under traditional agriculture: a comparison with wild populations of A. angustifolia (Agavaceae) and commercial plantations of A. tequilana. Am J Bot 96:448–457CrossRefPubMedGoogle Scholar
  68. Villaseñor JL, Dávila P, Chiang F (1990) Fitogeografía del Valle de Tehuacán-Cuicatlán. Bot Soc Bot Mex 50:135–149Google Scholar
  69. Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354CrossRefPubMedGoogle Scholar
  70. Zamora-Bustillos R, Rodríguez-Canul R, García de León FJ, Tello-Cetina J (2011) Diversidad genética de dos poblaciones de Strombus gigas (Gastropoda: Strombidae) de la Península de Yucatán, con microsatélites. Rev Biol Trop 59:1127–1134PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Lizeth I. Félix-Valdez
    • 1
  • Ofelia Vargas-Ponce
    • 1
  • Dánae Cabrera-Toledo
    • 1
  • Alejandro Casas
    • 2
  • Angélica Cibrian-Jaramillo
    • 3
  • Lino de la Cruz-Larios
    • 1
  1. 1.Centro Universitario de Ciencias Biológicas y AgropecuariasUniversidad de GuadalajaraNextipacMexico
  2. 2.Instituto de Investigaciones en Ecosistemas y Sustentabilidad (IIES)Universidad Nacional Autónoma de MéxicoMichoacánMexico
  3. 3.Laboratorio Nacional de Genómica para la BiodiversidadCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalGuanajuatoMexico

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