Phylogenetic relationship of 40 species of genus Aloe L. and the origin of an allodiploid species revealed by nucleotide sequence variation in chloroplast intergenic space and cytogenetic in situ hybridization
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Aloe species, which have been used as medicinal plants, belong to the Asphodelaceae family consisting of 530 species. In this study, genetic diversity and phylogenetic relationships among 40 Aloe species including a putative interspecies hybrid were analyzed using PCR band profiles from eight chloroplast intergenic space markers and nucleotide sequence diversity in the psbK–psbI intergenic region. A phylogenetic tree based on psbK–psbI sequences supported the revised classification of the genus Aloe as polyphyletic with several species be re-allocated into three genera Kumara, Aloidendron, and Aloiampelos. Further, the origin of the putative interspecies Aloe hybrid was characterized through molecular cytogenetics. Fluorescence and genomic in situ hybridization illustrated that the hybrid has a bimodal karyotype with a chromosome complement of 2n = 14, of which complementary halves were derived from two parental species, A. vera and A. arborescens. These findings revealed that the hybrid species was allodiploid. The phylogenetic analysis showed that A. arborescens was the maternal genome donor of the hybrid, as both have identical chloroplast genome sequences. We thus conclude that the allodiploid hybrid should be called A. arborescens × A.vera.
KeywordsAloe Allodiploid interspecific hybrid psbK–psbI Genetic diversity
This study was supported by the Next-Generation BioGreen 21 Program (No. PJ011030-01) of the Rural Development Administration and Kim Jeong Moon Aloe Co. Republic of Korea.
- Daru BH, Manning JC, Boatwright JS, Maurin O, Maclean N, Schaefer H, Kuzmina M, van der Bank M (2013) Molecular and morphological analysis of subfamily Alooideae (Asphodelaceae) and the inclusion of Chortolirion in Aloe. Taxon 62:62–76Google Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Grace OM, Klopper RR, Smith GF, Crouch NR, Figueiredo E, Ronsted N, Van Wykae (2013) A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae). Phytotaxa 76:7–14Google Scholar
- Harpke D, Peterson A, Hoffmann MH, Röser M (2006) Phylogenetic evaluation of chloroplast trnL–trnF DNA sequence variation in the genus Mammillaria (Cactaceae). Schlechtendalia 14:7–16Google Scholar
- Klopper RR, Van Wyk AE, Smith GF (2010) Phylogenetic relationships in the family Asphodelaceae (Asparagales). Biodivers Ecol 3:9–36Google Scholar
- Lee YS, Yang TJ, Park SU, Baek JH, Wu S, Lim KB (2011) Induction and proliferation of adventitious roots from Aloe vera leaf tissues for in vitro production of aloe-emodin. Plant Omics 4:190–194Google Scholar
- Park YI, Lee SK (2006) New perspectives on Aloe. In: Sung CK (ed) The history of Aloe. Springer, Berlin, pp 7–17Google Scholar
- Smith GF, Klopper RR, Figueiredo E, Crouch NR (2012) Aspects of the taxonomy of Aloe arborescens Mill. (Asphodelaceae: Alooideae). Bradleya 30:127–137Google Scholar