Genetic Resources and Crop Evolution

, Volume 62, Issue 7, pp 1021–1027 | Cite as

Analysis of the molecular diversity of Olea europaea in the Mediterranean Island of Malta

  • O. Mazzitelli
  • A. Calleja
  • D. Sardella
  • C. Farrugia
  • M. Zammit-Mangion
Research Article


The island of Malta is a small densely populated land mass located in the middle of the Mediterranean Sea. Genetic material from local olive tree varieties on the island was amplified using RAPD, 36 loci were subsequently generated and a dendrogram based on Jaccard’s similarity coefficient constructed. Analysis of clustering patterns indicated a high degree of genetic diversity (0.18–0.68), an early separation between the majority of the native varieties and more recently introduced varieties, supporting the idea of a history of limited genetic exchange and indicating a separate clustering for the majority of the ‘Malti’ and ‘Bidni’ varieties. Principal Component Analysis of banding patterns confirmed these clustering patterns and analysis of the incidence of bands for primers OPA-17, OPC-19, OPI-18 (vector correlation significance <0.01) as well as for OPAG-13, OPAN-15 (vector correlation between 0.01 and 0.05) showed strong correlation. Native Maltese varieties were characterised by a higher number of bands arising from the former group of primers indicating their use as a possible means to distinguish between local and imported varieties.


Genetic diversity Olea europaea RAPD 



Samples were provided by Mr Sam Cremona, Mr Marcel Loporto, Mr Michael Vella and Mr Saviour Vella. We thank Mr Sam Cremona, Dr Tim Gambin, Mr Saviour Vella for their insight on historical events. The research work carried out by Oriana Mazzitelli was partially funded by the Strategic Educational Pathways Scholarship (Malta) Scholarship and was part-financed by the European Union – European Social Fund (ESF). Research work carried out by Alan Calleja and Davide Sardella was funded through the University of Malta’s research funds.


  1. Bonanno A (1977) Distribution of Villas and some Aspects of the Maltese Economy in the Roman Period. J Fac Arts VI 4:73–81Google Scholar
  2. Borg J (1922) Cultivation and diseases of fruit trees in the Maltese Islands. Kessinger Publishing, WhitefishCrossRefGoogle Scholar
  3. Buhagiar J (2012) Perspectives on olive cultivation and processing in maltese roman antiquity. In: The Zejtuna Roman Villa—Research, Conservation, Management. Wirt iz-Zejtun Publication, Gutenberg Press Ltd, MaltaGoogle Scholar
  4. Diaz C, Trujillo I, Barrio E, Belaj A, Barranco D, Rallo L (2011) Centennial trees as a reservoir of genetic diversity. Ann Bot 108(5):797–807CrossRefGoogle Scholar
  5. Doyle JA, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  6. Fenech K (2009) Human-induced changes in the environment and landscapes of the Maltese Islands from the Neolithic to the 15th Century AD BAC International Series 1682 Archaeopress, Publishers of British Archaeological ReportsGoogle Scholar
  7. Galea G (2011) Olive Tree Varieties in the Maltese Islands—A morphological analysis. Unpublished Master thesis—University of MaltaGoogle Scholar
  8. Gambin T (2012) A drop in the Ocean: Malta’s Trade in olive oil during the Roman Period In The Zejtuna Roman Villa—Research, Conservation, Management. Wirt iz-Zejtun PublicationGoogle Scholar
  9. Godwin H (1961) Appendix I, Report on a pollen samples from the late Borg in-Nadur cistern at tal-Mejtin, in Report on the working of the Museum Department for the year 1961, p 8 Malta. Department of InformationGoogle Scholar
  10. Gonzalo Claros M, Crespillo R, Aguilar M, Canovas F (2000) DNA fingerprinting and classification of geographically related genotypes of olive tree (Olea europaea L.). Euphytica 116:131–142CrossRefGoogle Scholar
  11. Hughes K (1999) Persistent features from a Palaeo-Landscape: the ancient tracks of the Maltese Islands. Geogr J 165:62–78CrossRefGoogle Scholar
  12. Laghetti G, Scicluna-Spiteri A, Attard E, Perrino P, Cifarelli S, Hammer K (2004) Collecting crop genetic resources in the in the Mediterranean agricultural islands: the Maltese Archipelago. PGRN 139:11–16Google Scholar
  13. Mazzitelli O (2013) Genetic analysis of olive (Olea europaea L.) varieties in the Maltese Islands using DNA markers: method development and technique evaluation. Unpublished dissertation submitted to the University of MaltaGoogle Scholar
  14. Sesli M, Yegenoglu ED (2009) RAPD-PCR analysis of cultured type olives in Turkey. Afr J Biotechnol 8(15):3419–3423Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • O. Mazzitelli
    • 1
  • A. Calleja
    • 2
  • D. Sardella
    • 3
  • C. Farrugia
    • 2
  • M. Zammit-Mangion
    • 1
  1. 1.Department of Physiology and BiochemistryUniversity of MaltaMsidaMalta
  2. 2.Department of ChemistryUniversity of MaltaMsidaMalta
  3. 3.Department of Food Studies and Environmental HealthUniversity of MaltaMsidaMalta

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