Advertisement

Genetic Resources and Crop Evolution

, Volume 62, Issue 3, pp 377–385 | Cite as

Assessing genetic diversity of Egyptian hexaploid wheat (Triticum aestivum L.) using microsatellite markers

  • Khaled F. M. SalemEmail author
  • Marion S. Röder
  • Andreas Börner
Research Article

Abstract

Genetic diversity was investigated in a set of thirty-three hexaploid wheat genotypes originated from Egypt, using 17 wheat microsatellites, representatives of fifteen wheat chromosomes. In total, ninety-five alleles were detected among Egyptian wheats. For 17 polymorphic microsatellite markers, the number of alleles per locus varied from 3 for Xgwm261-2DS, Xgwm3-3DL and Xgwm631-7AS to 11 for Xgwm437-7DL, with a mean of 5.59 alleles per locus. The highest average number of alleles per locus was detected in the B genome with 6.00, compared to 5.67 and 5.00 for genomes D and A, respectively. The highest and the lowest average number of alleles per locus among the wheat homoeologous groups were observed in group 7 and 3 with 7.00 and 4.00, respectively. Gene diversity for 17 microsatellites loci varied from 0.339 for Xgwm631-7AS to 0.845 for Xtaglgap-1BS with an average of 0.653. However, the gene diversity for three genomes A, B and D was 0.549, 0.718 and 0.674, respectively. A significant correlation coefficient between gene diversity and the number of alleles per locus, genomes and homoeologous groups was high, r = 0.649, 0.988 and 0.272 (P < 0.01), respectively. The gene diversity increased as the number of alleles increased. Cluster analysis was conducted based on microsatellites data. Five groups can be distinguished by truncating the dendrogram at genetic similarity (gs) value of 0.48. The present study indicated the presence of high diversity in Egyptian wheat genotypes.

Keywords

Egyptian wheat Genetic diversity Hexaploid wheat (Triticum aestivum L.) Microsatellite markers 

References

  1. Akfirat FS, Uncuoglu AA (2013) Genetic diversity of winter wheat (Triticum aestivum L.) revealed by SSR markers. Biochem Genet 51:223–229CrossRefGoogle Scholar
  2. Al-Khanjari S, Hammer K, Buerkert A, Röder MS (2007) Molecular diversity of Omani wheat revealed by microsatellites: II. Hexaploid landraces. Genet Resour Crop Evol 54:1407–1417CrossRefGoogle Scholar
  3. Anderson JA, Churchill GA, Autrique JE, Sorrells ME, Tanksley SD (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186CrossRefPubMedGoogle Scholar
  4. Barrett BA, Kidwell KK, Fox PN (1998) Comparison of AFLP and pedigree-based genetic diversity assessment methods using wheat cultivars from the Pacific Northwest. Crop Sci 38:1271–1278CrossRefGoogle Scholar
  5. Ben Amer IM, Börner A, Röder MS (2001) Detection of genetic diversity in Libyan wheat genotypes using wheat microsatellite markers. Genet Resour Crop Evol 48:579–585CrossRefGoogle Scholar
  6. Bohn M, Utz HF, Melchinger AE (1999) Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs and SSRs and their use for predicting progeny variance. Crop Sci 39:228–237CrossRefGoogle Scholar
  7. Börner A, Chebotar S, Korzun V (2000) Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance. Theor Appl Genet 100:494–497CrossRefGoogle Scholar
  8. Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci for agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936CrossRefPubMedGoogle Scholar
  9. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMedCentralPubMedGoogle Scholar
  10. Christiansen MJ, Andersen SB, Ortiz R (2002) Diversity changes in an intensively bred wheat germplasm during the 20th century. Mol Breed 9:1–11CrossRefGoogle Scholar
  11. Cuadrado A, Schwarzacher T (1998) The chromosomal organization of simple sequence repeats in wheat and rye genomes. Chromosoma 107:587–594CrossRefPubMedGoogle Scholar
  12. Devos KM, Bryan GJ, Collins AJ, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252CrossRefPubMedGoogle Scholar
  13. Dreisigacker S, Zhang P, Warburton M, Van Ginkel M, Hoisington D, Bohn M, Melchinger A (2004) SSR and pedigree analyses of genetic diversity among CIMMYT wheat lines targeted to different mega environments. Crop Sci 44:381–388CrossRefGoogle Scholar
  14. Fu YB, Peterson GW, Yu JK, Gao L, Jia J, Richards KW (2006) Impact of plant breeding on genetic diversity of the Canadian hard red spring wheat germplasm as revealed by EST-derived SSR markers. Theor Appl Genet 112:1239–1247CrossRefPubMedGoogle Scholar
  15. Graner A, Ludwig WF, Melchinger AE (1994) Relationship among European barley germplasm, II: comparison of RFLP and pedigree data. Crop Sci 34:1199–1205CrossRefGoogle Scholar
  16. Hammer K (2000) Microsatellite markers: a new tool for distinguishing diploid wheat species. Genet Resour Crop Evol 47:497–505CrossRefGoogle Scholar
  17. Huang XQ, Börner A, Röder MS, Ganal MW (2002) Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor Appl Genet 105:699–707CrossRefPubMedGoogle Scholar
  18. Jantasuriyarat C, Vales MI, Watson CJW, Riera-Lizarazu O (2004) Identification and mapping of genetic loci affecting free-threshing habit and spike compactness in wheat (Triticum aestivum L.). Theor Appl Genet 108:261–273CrossRefPubMedGoogle Scholar
  19. Khlestkina EK, Röder MS, Efremova TT, Börner A, Shumny VK (2004) The genetic diversity of old and modern Siberian varieties of common spring wheat as determined by microsatellite markers. Plant Breed 123:122–127CrossRefGoogle Scholar
  20. Korzun V, Röder MS, Ganal MW, Worland AJ, Law CN (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theor Appl Genet 96:1104–1109CrossRefGoogle Scholar
  21. Laido G, Mangini G, Taranto F, Gadaleta A, Blanco A et al (2013) Genetic diversity and population structure of tetraploid wheats (Triticum turgidum L.) estimated by SSR, DArT and pedigree data. PLoS One 8(6):e67280. doi: 10.1371/journal.pone.0067280 CrossRefPubMedCentralPubMedGoogle Scholar
  22. Landjeva S, Korzun V, Ganeva G (2006) Evaluation of genetic diversity among Bulgarian winter wheat (Triticum aestivum L.) varieties during the period 1925–2003 using microsatellites. Genet Resour Crop Evol 53:1605–1614CrossRefGoogle Scholar
  23. Manifesto MM, Schlatter AR, Hop HE, Suarez EY, Dubcovsky J (2001) Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Sci 41:682–690CrossRefGoogle Scholar
  24. Mukhtar MS, Rehman M, Zafar Y (2002) Assessment of genetic diversity among wheat (T. aestivum L.) cultivars from a range of localities across Pakistan using random amplified polymorphic DNA (RAPD) analysis. Euphytica 28:417–425CrossRefGoogle Scholar
  25. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323CrossRefPubMedCentralPubMedGoogle Scholar
  26. Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273CrossRefPubMedCentralPubMedGoogle Scholar
  27. Ortiz R (2001) Germplasm enhancement to sustain genetic gains in crop improvement. In: Engels JMM, Ramanatha VR, Brown AHD, Jackson M (eds) Managing plant genetic diversity. IPGRI & CAB International, Rome, pp 275–290Google Scholar
  28. Peleg Z, Fahima T, Korol AB, Abbo S, Saranga Y (2011) Genetic analysis of wheat domestication and evolution under domestication. J Exp Bot 62:5051–5061CrossRefPubMedCentralPubMedGoogle Scholar
  29. Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007PubMedGoogle Scholar
  30. Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592Google Scholar
  31. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedCentralPubMedGoogle Scholar
  32. Röder MS, Wendehake K, Korzun V, Bredemeijer G, Laborie D, Bertrand L, Isaac P, Rendell S, Jackson J, Cooke RJ, Vosman B, Ganal MW (2002) Construction and analysis of a microsatellite-based database of European wheat varieties. Theor Appl Gent 106:67–73Google Scholar
  33. Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.1 Exeter, New YorkGoogle Scholar
  34. Roussel V, Koenig J, Beckert M, Balfourier F (2004) Molecular diversity in French bread wheat accessions related to temporal trends and breeding programmes. Theor Appl Gent 108:920–930CrossRefGoogle Scholar
  35. Roussel V, Leisova L, Exbrayat F, Stehno Z, Balfourier F (2005) SSR allelic diversity changes in 480 European bread wheat varieties released from 1840 to 2000. Theor Appl Genet 111:162–170CrossRefPubMedGoogle Scholar
  36. Salem KFM, El-Zanaty AM, Esmail RM (2008) Assessing (Triticum aestivum L.) wheat genetic diversity using morphological characters and microsatellite markers. World J Agric Sci 4(5):538–544Google Scholar
  37. Salem KFM, Röder MS, Börner A (2007) Identification and mapping quantitative trait loci for stem reserve mobilisation in wheat (Triticum aestivum L.). Cereal Res Common 35:1367–1374CrossRefGoogle Scholar
  38. Salem KFM, Varshney RK, Röder MS, Börner A (2010) EST-SSR based estimates on functional genetic variation in a barley (Hordeum vulgare L.) collection from Egypt. Genet Resour Crop Evol 57:515–525CrossRefGoogle Scholar
  39. Sonmezoglu OA, Bozmaz B, Yildirim A, Kandemir N, Aydin N (2012) Genetic characterization of Turkish bread wheat landraces based on microsatellite markers and morphological characters. Turk J Biol 36:589–597Google Scholar
  40. Sorrells ME, Wilson WA (1997) Direct classification and selection of superior alleles for crop improvement. Crop Sci 37:691–697CrossRefGoogle Scholar
  41. Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066CrossRefPubMedGoogle Scholar
  42. Tripp R (1996) Biodiversity and modern crop varieties: sharpening the debate. Agric Hum Values 13:48–62CrossRefGoogle Scholar
  43. Velle R (1993) The decline of diversity in European agriculture. Ecologist 23:64–69Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Khaled F. M. Salem
    • 1
    Email author
  • Marion S. Röder
    • 2
  • Andreas Börner
    • 2
  1. 1.Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI)Sadat City UniversitySadat CityEgypt
  2. 2.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany

Personalised recommendations