Genetic Resources and Crop Evolution

, Volume 61, Issue 5, pp 909–925 | Cite as

Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.

  • Behrouz Shiran
  • Soghra Kiani
  • Deepmala Sehgal
  • Akram Hafizi
  • Tanvir ul-Hassan
  • Manju Chaudhary
  • Soom Nath Raina
Research Article

Abstract

The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study.

Keywords

ITS1-5.8S-ITS2 Neighbor joining Nomenclature Parsimony Taxonomy Vicia L. 

Notes

Acknowledgments

Grateful thanks are due to National Academy of Sciences, India and Shahrekord University, Iran for financial support.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Behrouz Shiran
    • 1
  • Soghra Kiani
    • 2
  • Deepmala Sehgal
    • 3
  • Akram Hafizi
    • 1
  • Tanvir ul-Hassan
    • 4
  • Manju Chaudhary
    • 5
  • Soom Nath Raina
    • 5
  1. 1.Department of Plant Breeding and Biotechnology, Faculty of AgricultureShahrekord UniversityShahrekordIran
  2. 2.Department of AgriculturePayame Noor UniversityTehranIran
  3. 3.International Wheat and Maize Improvement Center (CIMMYT)TexcocoMexico
  4. 4.Department of BotanyUniversity of DelhiDelhiIndia
  5. 5.Amity Institute of BiotechnologyAmity UniversityNoidaIndia

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