Genetic Resources and Crop Evolution

, Volume 60, Issue 6, pp 1777–1800 | Cite as

Genetic variation and phylogenetic relationships among Indian Citrus taxa revealed by DAMD-PCR markers

  • Susheel Kumar
  • K. Narayanan NairEmail author
Research Article


Genetic variation and phylogenetic relationships among 50 wild and cultivated accessions of 19 Indian Citrus genotypes were examined through comparison of Directed Amplification of Minisatellite DNA (DAMD) markers and morphological characters. DAMD-PCR analysis with four primers resulted in amplification of a total of 45 bands, of which 35 (78 %) were polymorphic. Morphometric evaluation using 76 morphological characters showed high level of variability ranging from 0.18 to 1.00 (avg 0.39), whereas the Jaccard’s coefficient values of genetic similarity calculated from DAMD data ranged from 0.41 to 1.00 (avg 0.68), indicating moderate genetic divergence among the accessions studied. UPGMA dendrograms generated separately from morphometric and DAMD data segregated all the accessions of Citrus into four main clusters, each containing a true basic species and their probable hybrids. The grouping of individual accessions/genotypes under respective species or cultivars in DAMD dendrogram was based purely on their genetic relationships rather than geographical origin. There was no absolute congruence between the data and dendrograms generated from morphometric and DAMD analyses. The study demonstrates the resolving power of DAMD markers for discrimination of individual genotypes of Citrus under its respective species, hybrid or cultivar groups and inferring their genetic and phylogenetic relationships as well. This is the first report on application of DAMD markers in Citrus.


Citrus DAMD Genetic variation Morphometric characterization Phylogeny Polymorphism 



The authors thank the Director, CSIR-National Botanical Research Institute (CSIR-NBRI) for providing facilities to carry out this work, and the officers and staff of Nokrek Biosphere Reserve (NBR), Tura, Meghalaya for their help and support during exploration trips to NBR. The financial support from the Department of Biotechnology, New Delhi and the Council of Scientific and Industrial Research (CSIR), New Delhi is gratefully acknowledged.

Supplementary material

10722_2013_9954_MOESM1_ESM.pdf (112 kb)
Supplementary material 1 (PDF 112 kb)
10722_2013_9954_MOESM2_ESM.pdf (112 kb)
Supplementary material 2 (PDF 113 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Tissue Culture and Cryopreservation UnitNational Bureau of Plant Genetic ResourcesNew DelhiIndia
  2. 2.Plant Diversity, Systematics and Herbarium DivisionCSIR-National Botanical Research InstituteLucknowIndia

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