Genetic Resources and Crop Evolution

, Volume 60, Issue 3, pp 1153–1163 | Cite as

The use of microsatellite polymorphisms to characterise and compare genetic variability in Avena strigosa and A. barbata

  • Adrian L. Cabral
  • Haydar Karaoglu
  • Robert F. Park
Research Article

Abstract

Microsatellite (SSR) polymorphism was assessed across 90 diploid Avena strigosa Schreb. and tetraploid Avena barbata Pott ex Link accessions obtained from the USDA-ARS National Small Grains Collection using 105 genomic SSRs. Eleven polymorphic SSRs that detected 69 different alleles were identified and used to genotype the 90 accessions, which were chosen from a larger set of 385 accessions based on geographical source-diversity and variable reaction responses to five Australian pathotypes of the crown rust pathogen Puccinia coronata Corda f. sp. avenae Eriks. Eight diploid and eight tetraploid clades were identified among the 90 accessions. Diploid accessions displayed the lowest genetic diversity, with all accessions being at least 86 % similar, and included accessions from countries in the Americas such as Canada, USA, Argentina, Uruguay and Brazil, and European accessions from France, Romania and Poland. Although both species formed distinct clusters in the dendrogram, a few instances of diploids showing high similarity with tetraploids and vice versa were observed. An AMOVA analysis revealed 86 % of the total genetic variation to be distributed within the two oat species, while between-species differences accounted for only 14 %. Heterozygosity (H) index values of 0.32 and 0.40 were obtained for diploids and tetraploids respectively. Our study effectively differentiated A. strigosa and A. barbata, and identified 11 SSRs suitable for future characterisation of accessions of the two species.

Keywords

Avena strigosa A. barbata Analysis of molecular variance Genetic similarity SSR Simple sequence repeats 

Notes

Acknowledgments

The authors thank Dr. Celeste Linde for her valuable comments, and acknowledge funding from the Sir Alexander Hugh Thurburn Faculty Scholarship at the University of Sydney and the Australian Grains Research and Development Corporation. We are also most appreciative of the technical assistance provided by Mr. Paul Kavanagh.

Supplementary material

10722_2012_9911_MOESM1_ESM.docx (38 kb)
Supplementary material 1 (DOCX 37 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Adrian L. Cabral
    • 1
    • 2
  • Haydar Karaoglu
    • 1
  • Robert F. Park
    • 1
  1. 1.Plant Breeding Institute CobbittyThe University of SydneyNarellanAustralia
  2. 2.Agriculture and Agri-Food Canada, Cereal Research CentreWinnipegCanada

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