Genetic Resources and Crop Evolution

, Volume 59, Issue 6, pp 1161–1168 | Cite as

Characterization of a new T2DS.2DL-?R translocation triticale ZH-1 with multiple resistances to diseases

  • Jianping Zhou
  • Huaiyu Zhang
  • Zujun Yang
  • Guangrong Li
  • Lijun Hu
  • Mengping Lei
  • Cheng Liu
  • Yong Zhang
  • Zhenglong Ren
Research Article
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Abstract

In the present study, we report on a new triticale (×Triticosecale Wittm.) ZH-1, derived from the progeny of common wheat (Triticum aestivum L.) cv. MY15 × Chinese Weining rye (Secale cereale L.). ZH-1, exhibiting shorter plant height and higher tillering ability compared to MY15, is immune to both powdery mildew and stripe rust and has stable fertility. Genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and C-banding revealed that the chromosome composition of triticale ZH-1 was 14 A-genome (1A-7A), 12 B-genome (1B-2B, 4B-7B), 12 R-genome (1R, 3R-7R), chromosomes 6D and T2DS.2DL-?R. Moreover, the PCR results of PLUG and EST-SSR markers also strongly supported the above stated chromosome composition of triticale ZH-1. In addition, the physical mapping of chromosome T2DS.2DL-?R showed a minute chromosomal fragment derived from rye was attached at the distal end of 2DL. The new triticale ZH-1 could be a valuable source for wheat improvement, especially for resistance to disease.

Keywords

C-banding FISH GISH Triticale Wheat-rye small-segment-translocation 
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30800685, No. 30730065 and No. 30900779) and China Postdoctoral Science Foundation (No. 20090461333). We particularly thank Dr. Peigao Luo for his helpful revising the manuscript.

References

  1. Badaeva ED, Amosova AV, Muravenko OV, Samatadze TE, Chikida NN, Zelenin AV, Friebe B, Gill BS (2002) Genome differentiation in Aegilops. 3. Evolution of the D-genome cluster. Plant Syst Evol 231:163–190CrossRefGoogle Scholar
  2. Bernard S, Bernard M (1987) Creating new forms 4×, 6× and 8× primary Triticale associating both complete R and D genomes. Theor Appl Genet 74:55–59CrossRefGoogle Scholar
  3. Cainong JC, Zavatsky LE, Chen MS, Johnson J, Friebe B, Gill BS, Lukaszewski AJ (2010) Wheat-rye T2BS·2BL-2RL recombinants with resistance to Hessian fly (H21). Crop Sci 50:920–925CrossRefGoogle Scholar
  4. Dou QW, Tanaka H, Nakata N, Tsujimoto H (2006) Molecular cytogenetic analyses of hexaploid lines spontaneously appearing in octoploid Triticale. Theor Appl Genet 114:41–47PubMedCrossRefGoogle Scholar
  5. Endo TR, Yamamoto M, Mukai Y (1994) Structural changes of rye chromosome 1R induced by a gametocidal chromosome. Jpn J Genet 69:13–19CrossRefGoogle Scholar
  6. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterisation of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  7. Gill BS, Kimber G (1974) The Giemsa C-banded karyotype of rye. Proc Natl Acad Sci USA 71:1247–1249PubMedCrossRefGoogle Scholar
  8. Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34:830–839CrossRefGoogle Scholar
  9. Hammer K, Filatenko AA, Pistrick K (2011) Taxonomic remarks on Triticum L. and × Triticosecale Wittm. Genet Resour Crop Evol 58:3–10CrossRefGoogle Scholar
  10. Ishikawa G, Nakamura T, Ashida T, Saito M, Nasuda S, Endo TR, Wu J, Matsumoto T (2009) Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor Appl Genet 118(3):499–514PubMedCrossRefGoogle Scholar
  11. Jiang J, Hulbert HS, Gill BS, Ward DC (1996) Interphase fluorescence in situ hybridization: a physical mapping strategy for plant species with large complex genomes. Mol Gen Genet 252:497–502PubMedCrossRefGoogle Scholar
  12. Leach RC, Dundas IS, Houben A (2006) Construction of comparative genetic maps of two 4Bs.4Bl–5Rl translocations in bread wheat (Triticum aestivum L.). Genome 49:729–734PubMedCrossRefGoogle Scholar
  13. Lee TG, Hong MJ, Johnson JW, Bland DE, Kim DY, Seo YW (2009) Development and functional assessment of EST-derived 2RL-specific markers for 2BS.2RL translocations. Theor Appl Genet 119(4):663–673PubMedCrossRefGoogle Scholar
  14. Leonova IN, Dobrovolskaya OB, Kaminskaya LN, Adonina IG, Koren LV, Khotyljova LV, Salina EA (2005) Molecular analysis of the Triticale lines with diVerent Vrn gene systems using microsatellite markers and hybridization in situ. Russ J Genet 41(9):1014–1020CrossRefGoogle Scholar
  15. Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225CrossRefGoogle Scholar
  16. Lukaszewski AJ, Gustafson JP (1987) Cytogenetics of triticale. Plant Breed Rev 5:41–93Google Scholar
  17. Ma H, Singh RP, Mujeeb-Kazi A (1995) Suppression/expression of resistance to stripe rust in synthetic hexaploid wheat (Triticum turgidum × T. tauschii). Euphytica 83:87–93CrossRefGoogle Scholar
  18. Ma R, Yli-Mattila T, Pulli S (2004) Phylogenetic relationships among genotypes of worldwide collection of spring and winter ryes (Secale cereale L.) determined by RAPD-PCR markers. Hereditas 140:210–221PubMedCrossRefGoogle Scholar
  19. Martin DJ, Stewart BJ (1990) Dough stickiness in rye-derived wheat cultivars. Euphytica 51:77–86CrossRefGoogle Scholar
  20. Masoudi-Nejad A, Nasuda S, McIntosh RA, Endo TR (2002) Transfer of rye chromosome segments to wheat by a gametocidal system. Chromosom Res 10:349–357CrossRefGoogle Scholar
  21. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In: Appels R, Eastwood R, Lagudah E et al (eds) Proceedings of 11th international wheat genetics symposium (on CD). University of Sydney Press, AustraliaGoogle Scholar
  22. Müntzing A (1939) Studies on the properties and the ways of production of rye-wheat amphidiploids. Hereditas 25:387–430CrossRefGoogle Scholar
  23. O’Mara JG (1953) The cytogenetics of triticale. Bot Rev 19:587–605CrossRefGoogle Scholar
  24. Qi ZJ, Liu DJ, Chen PD, Li QQ (2001) Molecular cytogenetic analysis of winter wheat germplasm aimengniu. Acta Botanica Sinica 43(5):469–474Google Scholar
  25. Rayburn AL, Gill BS (1986) Molecular identification of the D-genome of wheat. J Hered 77:253–255Google Scholar
  26. Ribeiro-Carvalho C, Guedes-Pinto H, Heslop-Harrison JS, Schwarzacher T (2010) Introgression of rye chromatin on chromosome 2D in the Portuguese wheat landrace ‘Barbela’. Genome 44:1122–1128CrossRefGoogle Scholar
  27. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedCrossRefGoogle Scholar
  28. Schneider A, Molnár I, Molnár-Láng M (2008) Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163:1–19CrossRefGoogle Scholar
  29. Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69CrossRefGoogle Scholar
  30. Singh RP, Shepherd KW, McIntosh RA (1990) Linkage mapping of genes for resistance to leaf rust, stem and stripe rusts and v-secalins on the short arm of rye chromosome 1R. Theor Appl Genet 80:609–616CrossRefGoogle Scholar
  31. Tams SH, Bauer E, Oettler G, Melchinger AE (2004) Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient. Theor Appl Genet 108:1385–1391PubMedCrossRefGoogle Scholar
  32. Tikhenko ND, Tsvetkova NV, Voilokov AV (2003a) Analysis of the effect of the genotype of parental rye lines on quantitative trait formation in primary octoploid triticale: plant height. Russ J Genet 39:52–56CrossRefGoogle Scholar
  33. Tikhenko ND, Tsvetkova NV, Voilokov AV (2003b) The effect of parental genotypes of rye lines on the development of quantitative traits in primary octoploid triticale: spike fertility. Russ J Genet 39:295–299CrossRefGoogle Scholar
  34. Weimarck A (1974) Elimination of wheat and rye chromosomes in a strain of octoploid triticale as revealed by Giemsa banding technique. Hereditas 77:281–286PubMedCrossRefGoogle Scholar
  35. Wilson AS (1876) Wheat and rye hybrids. Edinb Bat Sac Trans 12:286–288CrossRefGoogle Scholar
  36. Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhu HL, Wu JZ, Xu HB, Zhao DM, Tian DG, Zhang CQ, Ma ZQ (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189PubMedCrossRefGoogle Scholar
  37. Zeller FJ, Stephan U, Lutz J (1993) Chromosomal location of powdery mildew resistance genes in common wheat (Triticum aestivum L.). 1. Mlk and other alleles in Pm3 locus. Euphytica 68:223–229CrossRefGoogle Scholar
  38. Zhou JP, Yang ZJ, Feng J, Zhang XZ, Ren ZL (2007) Morphological, cytogenetic and molecular identification of a new triticale. Cereal Res Commun 35(3):1385–1395CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jianping Zhou
    • 1
  • Huaiyu Zhang
    • 2
  • Zujun Yang
    • 1
  • Guangrong Li
    • 1
  • Lijun Hu
    • 1
  • Mengping Lei
    • 1
  • Cheng Liu
    • 1
  • Yong Zhang
    • 1
  • Zhenglong Ren
    • 1
    • 2
  1. 1.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduPeople’s Republic of China
  2. 2.State Key Laboratory for Plant Genetics and BreedingSichuan Agricultural UniversityYa’anPeople’s Republic of China

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