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Genetic Resources and Crop Evolution

, Volume 58, Issue 3, pp 425–437 | Cite as

Characterization of Italian grasspea (Lathyrus sativus L.) germplasm using agronomic traits, biochemical and molecular markers

  • Lucia LioiEmail author
  • Francesca Sparvoli
  • Gabriella Sonnante
  • Gaetano Laghetti
  • Francesco Lupo
  • Massimo Zaccardelli
Research Article

Abstract

Genetic relationships among 13 grasspea (Lathyrus sativus L.) landraces mainly collected in Southern Italy were assessed using agronomic traits, biochemical and molecular markers. Field trials were carried out in two locations and revealed a high influence of field locations on yield, but a low genotype × environment interaction. Despite this, the agronomic data obtained provided useful information for the choice of the best grasspea landraces for southern Italian marginal areas. Seed storage proteins utilised as biochemical markers were not able to detect polymorphisms, on the contrary both classes of molecular markers used i.e. AFLP and SSR, provided useful information on genetic variation and relationships among landraces. Even though the number of polymorphic fragments detected by AFLP technique was low, it was sufficient to discriminate all the accessions. The use of SSR to detect polymorphic sites in grasspea showed that most landraces were clearly grouped in two sub-clusters. One comprised two landraces from most northern localities, while all the other landraces were clustered together at a very narrow genetic distance.

Keywords

AFLP Field trials Genetic resources Italian grasspea Lathyrus sativus L. SSR 

Notes

Acknowledgments

Research partially supported by Ministry of Agriculture Food and Forestry Policies with funds released by C.I.P.E (Resolution 17/2003). We thank Maria Galiano and Giovanni Tagliabue for biochemical and molecular analyses support.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lucia Lioi
    • 1
    Email author
  • Francesca Sparvoli
    • 2
  • Gabriella Sonnante
    • 1
  • Gaetano Laghetti
    • 1
  • Francesco Lupo
    • 3
  • Massimo Zaccardelli
    • 3
  1. 1.CNR-Istituto di Genetica VegetaleBariItaly
  2. 2.CNR-Istituto di Biologia e Biotecnologia AgrariaMilanItaly
  3. 3.CRA-Centro di Ricerca per l’ Orticoltura, Azienda Sperimentale di BattipagliaBattipagliaItaly

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