Genetic Resources and Crop Evolution

, Volume 57, Issue 6, pp 841–851

Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus

  • Lihua Yao
  • Xiaoyan Zheng
  • Danying Cai
  • Yuan Gao
  • Kun Wang
  • Yufen Cao
  • Yuanwen Teng
Research Article

Abstract

A total of 8117 suitable SSR-contaning ESTs were acquired by screening from a Malus EST database, among which dinudeotide SSRs were the most abundant repeat motif, within which, CT/TC followed by AG/GA were predominant. Based on the suitable sequences, we developed 147 SSR primer pairs, of which 94 pairs gave amplifications within the expected size range while 65 pairs were found to be polymorphic after a preliminary test. Eighteen primer pairs selected randomly were further used to assess genetic relationship among 20 Malus species or cultivars. As a result, these primers displayed high level of polymorphism with a mean of 6.94 alleles per locus and UPGMA cluster analysis grouped twenty Malus accessions into five groups at the similarity level of 0.6800 that were largely congruent to the traditional taxonomy. Subsequently, all of the 94 primer pairs were tested on four accessions of Pyrus to evaluate the transferability of the markers, and 40 of 72 functional SSRs produced polymorphic amplicons from which 8 SSR loci selected randomly were employed to analyze genetic diversity and relationship among a collection of Pyrus. The 8 primer pairs produced expected bands with the similar size in apples with an average of 7.375 alleles per locus. The observed heterozygosity of different loci ranged from 0.29 (MES96) to 0.83 (MES138), with a mean of 0.55 which is lower than 0.63 reported in genome-derived SSR marker analysis in Pyrus. The UPGMA dendrogram was similar to the previous results obtained by using RAPD and AFLP markers. Our results showed that these EST-SSR markers displayed reliable amplification and considerable polymorphism in both Malus and Pyrus, and will contribute to the knowledge of genetic study of Malus and genetically closed genera.

Keywords

Expressed sequence tags (ESTs) Genetic diversity Malus Pyrus Simple sequence repeat (SSR) 

Supplementary material

10722_2009_9524_MOESM1_ESM.pdf (81 kb)
Supplementary material 1 (PDF 82 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lihua Yao
    • 1
    • 2
  • Xiaoyan Zheng
    • 1
    • 2
  • Danying Cai
    • 1
    • 2
  • Yuan Gao
    • 3
  • Kun Wang
    • 3
  • Yufen Cao
    • 3
  • Yuanwen Teng
    • 1
    • 2
  1. 1.Department of HorticultureZhejiang UniversityHangzhouChina
  2. 2.The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development & Quality ImprovementHangzhouChina
  3. 3.Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengChina

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