Genetic Resources and Crop Evolution

, Volume 57, Issue 3, pp 357–370 | Cite as

Identification of European and Asian pears using EST-SSRs from Pyrus

Research Article

Abstract

Ten EST-SSRs previously isolated from Pyrus were used to identify 81 P. communis, 13 P. pyrifolia and 20 P. ussuriensis or P. × bretschneideri accessions. Cross-transference of these EST-SSRs was high in these species. PYC-008 and PYC-004 were the least informative SSRs in each of the pear species and were monomorphic in P. pyrifolia while PYC-013, PYC-002 and PYC-009b were the most informative in all species. EST-SSRs were very valuable for identification of incorrectly identified accessions, failed grafts and sets of synonyms in each of the species. Unsuspected relationships were uncovered, including a parental relationship between ‘Anjou’ and ‘Farmingdale’, a clonal relationship between ‘Berger’ and ‘Bartlett’, and a very close relationship between ‘Beurre Superfin’ and ‘Doyenne du Comice’. One SSR marker was different in one of three sports of ‘Doyenne du Comice’ (‘Doyenne du Comice Crimson Gem’) and in one of two sports of ‘Anjou’ (‘Gebhard Red’ red skin sport of ‘Anjou’). UPGMA cluster analysis separated the pear accessions into a large European cluster and an Asian group mostly according to common ancestry, geographical origin or time of ripening. High cross-transference of EST-SSRs in Pyrus species is very valuable for germplasm management in such a highly diverse collection as found at the NCGR Pyrus genebank in Corvallis, OR.

Keywords

Asian pear European pear Expressed sequence tags (ESTs) Microsatellite markers Pyrus Simple sequence repeat (SSR) 

Notes

Acknowledgments

We acknowledge Barbara Gilmore, Christine Neou-Anderson, and April Nyberg for technical assistance in microsatellite marker separation. Funding for this study was provided by the USDA-ARS CRIS 5358-21000-033-00D and a USDA-ARS National Plant Germplasm System Evaluation Grant.

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Copyright information

© US Government 2009

Authors and Affiliations

  1. 1.United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Clonal Germplasm Repository (NCGR)CorvallisUSA

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