Genetic Resources and Crop Evolution

, Volume 56, Issue 4, pp 455–464

Genetic diversity and differentiation in Chinese sour cherry Prunus pseudocerasus Lindl., and its implications for conservation

  • Miao-Miao Li
  • Yu-Liang Cai
  • Zeng-Qiang Qian
  • Gui-Fang Zhao
Research Article

DOI: 10.1007/s10722-008-9378-y

Cite this article as:
Li, MM., Cai, YL., Qian, ZQ. et al. Genet Resour Crop Evol (2009) 56: 455. doi:10.1007/s10722-008-9378-y

Abstract

In this study, the genetic diversity and differentiation of 10 natural Prunus pseudocerasus Lindl. populations were investigated using inter-simple sequence repeat (ISSR) markers. Totally, 18 selected primers generated 150 loci, with an average of 8.33 bands per primer. The results showed that the percentage of polymorphic bands (PPB) was pretty low at the population level (PPB = 1.13–32%), but relatively high at the species level (PPB = 84%). Besides, a high level of genetic differentiation among populations was detected based on the gene differentiation coefficient (GST = 0.7118) and the hierarchical analysis of molecular variance (AMOVA) (ΦST = 64.53%, P < 0.001), in line with the low inter-population gene flow (Nm = 0.2025). Moreover, Mantel test revealed a significant correlation between genetic and geographic distances among the populations (r = 0.5272, P < 0.005). The high level of intraspecific genetic diversity was probably related with its life history traits, while its small population size and the resultant high levels of genetic drift and inbreeding might explain the low genetic diversity within populations. The relatively high inter-population genetic differentiation was largely attributed to its small population size, habitat fragmentation, the mode of pollen and seed dispersal, and geographic isolation. Based on the present study, conservation strategies were proposed to preserve this valuable natural germplasm resource.

Keywords

Cherry Genetic differentiation Genetic diversity Inter-simple sequence repeats (ISSRs) Prunus pseudocerasus Lindl. 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Miao-Miao Li
    • 1
  • Yu-Liang Cai
    • 2
  • Zeng-Qiang Qian
    • 3
  • Gui-Fang Zhao
    • 1
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life SciencesNorthwest UniversityXianPeople’s Republic of China
  2. 2.College of HorticultureNorthwest A & F UniversityYanglingPeople’s Republic of China
  3. 3.School of Marine and Tropical Biology and Comparative Genomics CentreJames Cook UniversityTownsvilleAustralia

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