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Genetic Resources and Crop Evolution

, Volume 55, Issue 4, pp 481–485 | Cite as

Results of molecular analysis of an archaeological hemp (Cannabis sativa L.) DNA sample from North West China

  • Ashutosh Mukherjee
  • Satyesh Chandra Roy
  • S. De Bera
  • Hong-En Jiang
  • Xiao Li
  • Cheng-Sen Li
  • Subir Bera
Short Communication

Abstract

Hemp (Cannabis sativa L.) cultivation and utilization is an ancient practice to human civilization. There are some controversies on the origin and subsequent spread of this species. Ancient plant DNA has proven to be a powerful tool to solve phylogenetic problems. In this study, ancient DNA was extracted from an archaeological specimen of Cannabis sativa associated with archaeological human remains from China. Ribosomal and Cannabis specific chloroplast DNA regions were PCR amplified. Sequencing of a species-specific region and subsequent comparison with published sequences were performed. Successful amplification, sequencing and sequence comparison with published data suggested the presence of hemp specific DNA in the archeological specimen. The role of Humulus japonicus Sieb. et Zucc. in the evolution of Cannabis is also indicated. The identification of ancient DNA of 2500 years old C. sativa sample showed that C. sativa races might have been introduced into China from the European–Siberian center of diversity.

Keywords

Archaeological DNA Hemp phylogeny Humulus japonicus Species identification Yanghai Tombs 

References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410PubMedGoogle Scholar
  2. Banerjee M, Brown TA (2002) Preservation of nuclear but not chloroplast DNA in archaeological assemblages of charred wheat grains. Anc Biomol 4(2):59–63CrossRefGoogle Scholar
  3. Blatter RHE, Jacomet S, Schlumbaum A (2002) Little evidence for the preservation of a single-copy gene in charred archaeological wheat. Anc Biomol 4(2):65–77CrossRefGoogle Scholar
  4. Bouquet RJ (1950) Cannabis. Bull Narc 2:14–30Google Scholar
  5. Crombie J, Crombie WML (1975) Cannabinoid formation in Cannabis sativa grafted interracially, and with two Humulus species. Phytochemistry 14:409–412CrossRefGoogle Scholar
  6. de Lamarck JB (1785) Encyclopédique Méthodique de Botanique, vol 1, Pt 2. Paris, FranceGoogle Scholar
  7. Faeti V, Mandolino G, Ranalli P (1996) Genetic diversity of Cannabis sativa germplasm based on RAPD markers. Plant Breed 115:367–370CrossRefGoogle Scholar
  8. Hillig KW (2005) Genetic evidence for speciation in Cannabis (Cannabaceae). Genet Resour Crop Evol 52:161–180CrossRefGoogle Scholar
  9. Jiang H-E, Li X, Zhao Y-X, Ferguson DK, Hueber F, Bera S, Wang Y-F, Zhao L-C, Liu C-J, Li C-S (2006) A new insight into Cannabis sativa (Cannabaceae) utilization from 2500-year-old Yanghai Tombs, Xinjiang, China. J Ethnopharmacol 108:414–422PubMedCrossRefGoogle Scholar
  10. Kim S, Soltis DE, Soltis PS, Suh Y (2004) DNA sequence of Miocene fossils: an ndhF sequence of Magnolia latahensis (Magnoliaceae) and an rbcL sequence of Persea pseudocarolinensis (Lauraceae). Am J Bot 91(4):615–620CrossRefGoogle Scholar
  11. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163PubMedCrossRefGoogle Scholar
  12. Li HL (1974) An archaeological and historical account of Cannabis in China. Econ Bot 28:437–448Google Scholar
  13. Li HL (1978) Hallucinogenic plants in Chinese herbals. J Psychedelic Drugs 10:17–26Google Scholar
  14. Liepelt S, Sperisen C, Deguilloux M-F, Petit RJ, Kissling R, Spencer M, De Beaulieu J-L, Taberlet P, Gielly L, Ziegenhagen B (2006) Authenticated DNA from ancient wood remains. Ann Bot 98:1107–1111PubMedCrossRefGoogle Scholar
  15. Linacre A, Thorpe J (1998) Detection and identification of Cannabis by DNA. Forensic Sci Int 91:71–76PubMedCrossRefGoogle Scholar
  16. Parducci L, Suyama Y, Lascoux M, Bennett KD (2005) Ancient DNA from pollen: a genetic record of population history in Scots pine. Mol Ecol 14:2873–2882PubMedCrossRefGoogle Scholar
  17. Pillay M, Kenny ST (2006) Structural organization of the nuclear ribosomal RNA genes in Cannabis and Humulus (Cannabaceae). Pl Syst Evol 258:97–105CrossRefGoogle Scholar
  18. Schultes RE (1970) Random thoughts and queries on the botany of Cannabis. In: Joice CRB, Curry SH (eds) The botany and chemistry of Cannabis. J. and A. Churchill Publishers, London, pp 11–38Google Scholar
  19. Schultes RE, Klein WM, Plowman T, Lockwood TE (1974) Cannabis, an example of taxonomic neglect. Bot Mus Leafl Harv Univ 23:337–367Google Scholar
  20. Small E, Cronquist A (1976) A practical and natural taxonomy for Cannabis. Taxon 25:405–435CrossRefGoogle Scholar
  21. Suyama Y, Kawamuro K, Kinoshita I, Yoshimura K, Tsumura Y, Takahara H (1996) DNA sequence from fossil pollen of Abies spp. from pleistocene fossils. Genes Genet Syst 71:145–149PubMedCrossRefGoogle Scholar
  22. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  23. Touw M (1981) The religious and medicinal uses of Cannabis in China, India and Tibet. J Psychoactive Drugs 13:23–34PubMedGoogle Scholar
  24. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innes M, Gelfand D, Sninsky J, White TJ (eds) A guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  25. Zeven AC, Zhukovsky PM (1975) Cannabidaceae. In: Dictionary of cultivated plants and their centres of diversity. Centre for Agricultural Publishing and Documentation, Wageningen, The Netherlands, pp 62–63, 129–130Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ashutosh Mukherjee
    • 1
  • Satyesh Chandra Roy
    • 2
  • S. De Bera
    • 2
  • Hong-En Jiang
    • 3
    • 4
  • Xiao Li
    • 5
  • Cheng-Sen Li
    • 3
    • 6
  • Subir Bera
    • 2
  1. 1.Department of BotanyDinabandhu MahavidyalayaBongaon, North 24 ParganasIndia
  2. 2.Department of BotanyUniversity of CalcuttaKolkataIndia
  3. 3.Laboratory of Systematic and Evolutionary BotanyChinese Academy of SciencesBeijingChina
  4. 4.Graduate SchoolChinese Academy of SciencesBeijingChina
  5. 5.Bureau of Cultural Relics of Turpan PrefectureXinjiangChina
  6. 6.Beijing Museum of Natural HistoryBeijingChina

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