Cross-amplification and characterization of microsatellite loci for three species of Theobroma (Sterculiaceae) from the Brazilian Amazon
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This study reports on the cross-species amplification of 23 microsatellite markers previously developed for Theobroma cacao L. (Sterculiaceae), source of chocolate in three economically important Amazonian species of Theobroma (T. grandiflorum, T. subincanum, T. sylvestre). Thirteen of the 23 microsatellite loci tested were polymorphic across the three species at 2–13 alleles per locus. The observed heterozygosity per locus varied from 0.18 to 0.84 and expected heterozygosity ranged from 0.28 to 0.87. The high level of transferability and genetic information content of these microsatellite loci indicate their usefulness for population genetic, mating system and breeding studies of these economically important Amazonian fruit trees.
KeywordsAmazon Cocoa Genetic diversity SSR Transferability Tropical tree
Financial support was provided by CNPq-Brazil (Grant no. 469926/2000-7 to RG), PROBIO/MMA-Brazil (Project contract # 0114-00/04), and European Union (GENEO-TROPECO Project contract # ERBIC18CT970149). We would like to thank Chris Dick and Jimmy Grogan for revision of the manuscript.
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