Evaluating landraces of bread wheat Triticum aestivum L. for tolerance to aluminium under low pH conditions
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Bread wheat (Triticum aestivum L.) landraces held within ex situ collections offer a valuable and largely unexplored genetic resource for wheat improvement programs. To maximise full utilisation of such collections the evaluation of landrace accessions for traits of interest is required. In this study, 250 accessions from 21 countries were screened sequentially for tolerance to aluminium (Al) using haematoxylin staining of root tips and by root regrowth measurement. The staining test indicated tolerance in 35 accessions, with an intermediate response to Al exhibited in a further 21 accessions. Of the 35 accessions classified as tolerant, 33 also exhibited increased root length following exposure to Al. The tolerant genotypes originated from Bulgaria, Croatia, India, Italy, Nepal, Spain, Tunisia, and Turkey. AFLP analysis of the 35 tolerant accessions indicated that these represent diverse genetic backgrounds. These accessions form a valuable set of germplasm for the study of Al tolerance and may be of benefit to breeding programs for expanding the diversity of the gene pool from which tolerant cultivars are developed.
KeywordsAFLP Aluminium tolerance Haematoxylin staining Landraces Root regrowth Triticum aestivum L.
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The authors wish to acknowledge the financial support given under the Preservation of Biological Assets program by the BioFirst initiative of the New South Wales Government, Australia.
- Aniol A, Gustafson JP (1984) Chromosome location of genes controlling aluminium tolerance in wheat, rye and triticale. Can J Genet Cytol 26:701–705Google Scholar
- Bennet RJ, Breen CM (1991) The aluminium signal: new dimensions to mechanisms of aluminium tolerance. Plant Soil 134:153–166Google Scholar
- Carver BF, Ownby JD (1995) Acid soil tolerance in wheat. In: Sparks DL (ed) Advances in agronomy. Academic Press Inc., San Diego, pp 117–173Google Scholar
- Coombes NE (2002) The reactive tabu search for efficient correlated experimental designs. PhD Thesis, Liverpool John Moores University, Liverpool, UKGoogle Scholar
- Felsenstein J (2002) PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USAGoogle Scholar
- Foy CD (1983) The physiology of plant adaptation to mineral stress. Iowa State J Res 57:355–392Google Scholar
- Gallardo F, Borie F, Alvear M, von-Baer E (1999) Evaluation of aluminium tolerance of three barley cultivars by two short-term screening methods and field experiments. Soil Sci Plant Nutr 45:713–719Google Scholar
- Kihara H (1983) Origin and history of ‘Daruma’, a parental variety of Norin 10. In: Sakamoto S (ed) Proc. 6th Int. Wheat Genetics Symp., Kyoto University Press, Kyoto, Japan, November 28–December 3, 1983, pp 13–19Google Scholar
- Kohli MM, Rajaram S (1988) Wheat breeding for acid soils: review of Brazilian/CIMMYT collaboration, 1974–1986. CIMMYT, Mexico, DFGoogle Scholar
- NLWRA (2001) National Land and Water Resources Audit. The Natural Heritage Trust, Commonwealth of AustraliaGoogle Scholar
- Raman H, Raman R, Tolhurst R, Martin P (2006) Repetitive indel markers within the ALMT-1 gene controlling aluminium tolerance in wheat (Triticum aestivum L.). SubmitedGoogle Scholar
- Raman H, Zhang K, Cakir M, Appels R, Garvin DF, Maron LG, Kochian LV, Moroni JS, Raman R, Imtiaz M, Drake-Brockman F, Waters I, Martin P, Sasaki T, Yamamoto Y, Matsumoto H, Hebb DM, Delhaize E, Ryan PR (2005) Molecular characterization and mapping of ALMT1, the aluminium-tolerance gene of bread wheat (Triticum aestivum L.). Genome 48:781–791PubMedGoogle Scholar
- Sneath PHA, Sokal RR (eds) (1973) Numerical taxonomy. The principles and practice of numerical classification. WH Freeman and Co., San FranciscoGoogle Scholar
- SoE (2001) State of the environment report prepared by A Hamblin. Department for the Environment, Canberra, ACT, AustraliaGoogle Scholar