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Genetic Resources and Crop Evolution

, Volume 54, Issue 7, pp 1437–1446 | Cite as

AFLP fingerprinting of sesame (Sesamum indicum L.) cultivars: identification, genetic relationship and comparison of AFLP informativeness parameters

  • Hernán LaurentinEmail author
  • Petr Karlovsky
Research Paper

Abstract

Amplified fragments length polymorphism (AFLP) was used to distinguish 20 cultivars of sesame (Sesamum indicum L.) and to elucidate the genetic relationship among these genotypes. The data were also used to estimate the usefulness of parameters currently used to assess the informativeness of molecular markers. A total of 339 markers were obtained using 8 primer combinations. Of the bands, 91% were polymorphic. Five primer combinations were able to distinguish all 20 cultivars used. None of the remaining three primer combinations could distinguish all accessions if used alone, but using all three combinations reduced the probability of a random match to 5 × 10−5. Polymorphic information content (PIC), resolving power (Rp) and marker index (MI) of each primer combination failed to correlate significantly with the number of genotypes resolved. Jaccard’s similarity coefficients ranged from 0.31 to 0.78. Fifteen cultivars were grouped by four UPGMA-clusters supported by bootstrapping values larger than 0.70. The grouping pattern was similar to the grouping generated by principal coordinate analysis. The results demonstrated that AFLP-based fingerprints can be used to identify unequivocally sesame genotypes, which is needed for cultivar identification and for the assessment of the genetic variability of breeding stocks. We recommend to use the number of cultivars identified by a primer combination instead of PIC, Rp and MI; and to calculate the maximal, instead of average probability of identical match by chance in the assessment of the informativeness of a marker for cultivar identification.

Keywords

AFLP DNA fingerprinting Genotype identification Genotyping Sesamum indicum 

Notes

Acknowledgements

This work was supported by the Programme Alban, European Union Programme of High Level Scholarships for Latin America, Identification Number E03D13301VE, International PhD program for Agricultural Sciences in Göttingen University (IPAG) and Universidad Centroccidental Lisandro Alvarado.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Department of Biologic Sciences, Agronomy FacultyUniversidad Centroccidental Lisandro AlvaradoCabudare, estado LaraVenezuela
  2. 2.Department of Crop SciencesGeorg-August UniversityGoettingenGermany

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