Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess.
Artemisia pallens Bess. is a low volume and high value essential oil plant used in perfumery, cosmetic and flavouring industries. On account of the failure of conventional procedures to induce variability in species, mutation techniques have been tried in our experiments. Dry and viable seeds (moisture content 8%) of homozygous pure breeding lines were subjected to 150–500 Gy doses of gamma rays and 0.01–0.1% ethyl methane sulphonate (EMS) for 8 h. Desirable qualitative mutants were recovered from segregating M2 generation (4,283 plants scored) raised as single plant progenies. The spectrum of morphological mutants included late and early flowering types; bushy and high yielding types; tall and more capitula-producing types and high oil and high davanone yielding types. These were raised through M3 families to evaluate stability and transmission of mutant characters. As such out of 15 different types selected in M2, only 11 types bred true to their characteristic variability. Based on their performance, the mutants were characterised depending upon their distinguishing features. Davanone, the main component of oil showed the maximum increase (64.22% against 54.64% in control) in mutant ‘S–5’ recovered from exposure with 250 Gy γ-rays. Mutant ‘E-6’ was economically most viable having increased oil biosynthesis (0.36% against 0.22% in control) and hence yields higher oil per unit area than the parental control (isolated from 0.05% EMS treatment).
KeywordsArtemisia pallens Characterisation Davanone EMS γ-rays Mutants
The first author thanks Dr A.K. Dhar Chairman, Plant Breeding and Crop Husbandry Division and Dr G.N. Qazi Director, Regional Research Laboratory, Jammu for providing facilities and their keen interest during course of study.
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