Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess.
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Artemisia pallens Bess. is a low volume and high value essential oil plant used in perfumery, cosmetic and flavouring industries. On account of the failure of conventional procedures to induce variability in species, mutation techniques have been tried in our experiments. Dry and viable seeds (moisture content 8%) of homozygous pure breeding lines were subjected to 150–500 Gy doses of gamma rays and 0.01–0.1% ethyl methane sulphonate (EMS) for 8 h. Desirable qualitative mutants were recovered from segregating M2 generation (4,283 plants scored) raised as single plant progenies. The spectrum of morphological mutants included late and early flowering types; bushy and high yielding types; tall and more capitula-producing types and high oil and high davanone yielding types. These were raised through M3 families to evaluate stability and transmission of mutant characters. As such out of 15 different types selected in M2, only 11 types bred true to their characteristic variability. Based on their performance, the mutants were characterised depending upon their distinguishing features. Davanone, the main component of oil showed the maximum increase (64.22% against 54.64% in control) in mutant ‘S–5’ recovered from exposure with 250 Gy γ-rays. Mutant ‘E-6’ was economically most viable having increased oil biosynthesis (0.36% against 0.22% in control) and hence yields higher oil per unit area than the parental control (isolated from 0.05% EMS treatment).
KeywordsArtemisia pallens Characterisation Davanone EMS γ-rays Mutants
The first author thanks Dr A.K. Dhar Chairman, Plant Breeding and Crop Husbandry Division and Dr G.N. Qazi Director, Regional Research Laboratory, Jammu for providing facilities and their keen interest during course of study.
- Farooqi AA, Dasharatha Rao ND, Devaiah KA, Ravi Kumar RL (1990) Genetic variability in davana (Artemisia pallens). Ind Perfum 34(1):42–42Google Scholar
- Gaikwad NB, Kothekar VS (2003) Induced morphological mutants in Lens culinaris. J Cytol Genet 4(NS):99–105Google Scholar
- Hefendehl FW, Murray MJ (1976) Genetic aspects of biosynthesis of natural odors. Lloydia 39(1):39–52Google Scholar
- Hegnauer R (1975) Secondary metabolites and crop plants. In: Frankel OH, Hawkes JG (eds) Crop genetic resources for today and tomorrow. Cambridge University Press, London, pp 249–255Google Scholar
- Jana MK (1963) X-ray induced mutants of Phaseolus mungo L. II. Sterility and vital mutants. Genet Iber 14:71–104Google Scholar
- Jeffrey C (2001) Compositae (Asteraceae). In: Hanelt P, Institute of plant genetics and crop plant research (eds) Mansfeld’s encyclopedia of agricultural and horticultural crops, vol 4. Springer, Berlin, pp 2035–2145Google Scholar
- Jhunjhunwalla A (2006) Market report of natural essential oils of Indian origin (as on 4th March, 2006). Ind Perfum 50(1):27Google Scholar
- Kak SN, Kaul BL (1980) Radiation induced useful mutants of Japanese mint (Mentha arvensis). Z. Pflanzen- zϋchtung 85:170–174Google Scholar
- Kaul BL, Singh C, Zutshi U, Dhar KL (1973) Radiation effects on growth and concentration of total alkaloids in Datura metel L. Ind J Exp Biol 11:133–134Google Scholar
- Levy A (1982) Natural and induced genetic variation in the biosynthesis of alkaloids and secondary metabolites. In: Improvement of oil seed and industrial crops by induced mutations. IAEA, Vienna, pp 213–222Google Scholar
- Mungikar AM (1997) An introduction to Biometry. Saraswati Printing Press, Motikaranja AurangabadGoogle Scholar
- Pawar SE, Wanjari KB (1994) Breeding high yielding varieties of pigeon pea, mungbean and black gram using induced mutations. In: DAE/BRNS Symp. Nuclear Applications in Agriculture, Animal Husbandry and Food Preservation. NRL IARI, New Delhi. pp 7–8Google Scholar
- Pawar SE, Thakar RG, Joshu DC (1979) Early maturing bold seeded mutant in pigeon pea (Cajanus cajan L). Millsp. Curr Sci 48:648–645Google Scholar
- Rekha K (1999) Mutation studies in Artemisia pallens Wall. Ph.D. Thesis, University of Jammu, JammuGoogle Scholar
- Rekha K, Kak SN (1997) Radiation induced variability in Artemisia pallens Wall. in M1 generation. J Econ Tax Bot 21(2):463–466Google Scholar
- Rekha K, Kak SN, Langer A (2000) EMS induced variability in Artemisia pallens Wall. Indian J Plant Genet Resour 13(1):37–41Google Scholar
- Sadowska A (1975) Effect of gamma ionizing radiation upon the yield of peppermint and on the quality of its essential oil. Proc. Polish Acad. Sci. Warsaw, pp 50Google Scholar
- Sengupta S, Datta AK (2004) Induced protein rich late flowering and seed coat colour mutants in sesame (Sesamum indicum L.). J Cytol Genet 5(NS):27–31Google Scholar
- Thakur RS, Misra LN (1989) Essential oils of Indian Artemisia. Proc. 11th International Congr. Essent. Oils, Frag. And Flavours, New Delhi, pp 127–135Google Scholar