Genetic Resources and Crop Evolution

, Volume 54, Issue 3, pp 519–527 | Cite as

Identification of duplicates and fingerprinting of primary and secondary wild annual Cicer gene pools using AFLP markers

  • F. Shan
  • H. J. Clarke
  • G. Yan
  • J. A. Plummer
  • K. H. M. Siddique


Wild annual Cicer gene pools contain valuable germplasm for chickpea improvement programs. Previous research showed that duplication might exist in accessions collected from these gene pools, which would hinder chickpea breeding and related research. AFLP (amplified fragment length polymorphism) markers were used to fingerprint the world collections of the primary and secondary gene pools including C. reticulatum Lad., C. bijugum K.H. Rech., C. judaicum Boiss. and C. pinnatifidum Jaub. et Sp. Duplicates were detected in a total of 24 accessions in both the gene pools, highlighting the necessity to fingerprint the germplasm. Genotypic difference was detected as gene pool specific, species specific and accession specific AFLP markers. These were developed into fingerprinting keys for accession identification between and within species and gene pools. Use of AFLP markers to detect duplicates and to identify accessions is a reliable method which will assist in the characterisation and use of wild annual Cicer germplasm in chickpea improvement programs. We recommend the procedure presented in this paper as a standard approach for the precise genetic identification and characterisation of future world collections of wild Cicer, to keep germplasm integrity and to benefit chickpea breeding and related research programs.

Key words

AFLP Chickpea Cicer bijugum Cicer judaicum Cicer pinnatifidum Cicer reticulatum Duplicate detection Germplasm Legume 


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Copyright information

© Springer 2006

Authors and Affiliations

  • F. Shan
    • 1
  • H. J. Clarke
    • 1
  • G. Yan
    • 1
    • 2
  • J. A. Plummer
    • 1
    • 2
  • K. H. M. Siddique
    • 1
  1. 1.Centre for Legumes in Mediterranean Agriculture, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawley Australia
  2. 2.School of Plant Biology, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawley Australia

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