Identification of duplicates and fingerprinting of primary and secondary wild annual Cicer gene pools using AFLP markers
Wild annual Cicer gene pools contain valuable germplasm for chickpea improvement programs. Previous research showed that duplication might exist in accessions collected from these gene pools, which would hinder chickpea breeding and related research. AFLP (amplified fragment length polymorphism) markers were used to fingerprint the world collections of the primary and secondary gene pools including C. reticulatum Lad., C. bijugum K.H. Rech., C. judaicum Boiss. and C. pinnatifidum Jaub. et Sp. Duplicates were detected in a total of 24 accessions in both the gene pools, highlighting the necessity to fingerprint the germplasm. Genotypic difference was detected as gene pool specific, species specific and accession specific AFLP markers. These were developed into fingerprinting keys for accession identification between and within species and gene pools. Use of AFLP markers to detect duplicates and to identify accessions is a reliable method which will assist in the characterisation and use of wild annual Cicer germplasm in chickpea improvement programs. We recommend the procedure presented in this paper as a standard approach for the precise genetic identification and characterisation of future world collections of wild Cicer, to keep germplasm integrity and to benefit chickpea breeding and related research programs.
Key wordsAFLP Chickpea Cicer bijugum Cicer judaicum Cicer pinnatifidum Cicer reticulatum Duplicate detection Germplasm Legume
Unable to display preview. Download preview PDF.
- Cansian R.L. and Echeverrigaray S. (2000). Discrimination among cultivars of cabbage using randomly amplified polymorphic DNA markers. Hortscience 35: 1155–1158Google Scholar
- Clarke H.J., Kuo I., Kuo J. and Siddique K.H.M. (2004). Abortion and stages for embryo rescue following wide crosses between chickpea (Cicer arietinum L.) and C. bijugum K.H. Rech. In: (eds) Legumes for the Benefit of AgricultureNutrition and the environment (5th European Conference on Grain Legumes and 2nd International Conference on Legume Genomics and Genetics), 7–11 June 2004, pp 193. Dijon, FranceGoogle Scholar
- Cooke R.J. (1999). Modern methods for cultivar verification and the transgenic plant challenge. Seed Sci. Technol. 27: 669–680Google Scholar
- Knights T., Brinsmead B., Fordyce M., Wood J., Kelly A. and Harden S. (2002). Use of the wild relative Cicer echinospermum in chickpea improvement. In: McComb, J.A. (eds) Proceedings of the 12th Australasian Plant Breeding Conference15–20th September, 2002, pp 150–154. Australasian Plant Breeding Assoc. Inc, Perth, W. AustraliaGoogle Scholar
- Kumar P.P., Yau J.C.K. and Goh C.J. (1998). Genetic analyses of Heliconia species and cultivars with randomly amplified polymorphic DNA (RAPD) markers. J. Am. Soc. Hortic. Sci. 123: 91–97Google Scholar
- Noli E., Conti S., Maccaferri M. and Sanguineti M.C. (1999). Molecular characterization of tomato cultivars. Seed Sci. Technol. 27: 1–10Google Scholar
- Robertson L.D., Singh K.B. and Ocampo B. (1995). A Catalog of Annual Wild Cicer Species. International Center for Agricultural Research in the Dry Areas (ICARDA). Aleppo, SyriaGoogle Scholar
- Winter P., Pfaff T., Udupa S.M., Huettel B., Sharma P.C., Sahi S., Arreguin-Espinoza R., Weigand F., Muehlbauer F.J. and Kahl G. (1999). Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol. Gen. Genet. 262: 90–101PubMedCrossRefGoogle Scholar
- Yadav S.S., Turner N.C. and Kumar J. (2002). Commercialization and utilization of wild genes for higher productivity in chickpea. In: McComb, J.A. (eds) Proceedings of the 12th Australasian Plant Breeding Conference15–20th September, 2002, pp 155–160. Australasian Plant Breeding Assoc. Inc, Perth, W. AustraliaGoogle Scholar