Genetic Resources and Crop Evolution

, Volume 53, Issue 8, pp 1553–1562 | Cite as

Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.

  • Atsushi MurakamiEmail author
  • Peter Darby
  • Branka Javornik
  • Maria S. S. Pais
  • Elisabeth Seigner
  • Anton Lutz
  • Petr Svoboda


To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.

Key words

Genetic diversity Humulus lupulus Microsatellite DNA Phylogeny 


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  1. H.J. Barth, C. Klinke and C. Schmidt, The Hop Atlas, the History and Geography of the Cultivated Plant. Nürnberg: Joh. Barth & Sohn (1994).Google Scholar
  2. Belkhir K.P., Borsa P., Chikhi L., Raufaste N. and Bonhomme F. 2001. GENETIX 4.02, logiciel sous Windows TM pour la génétique des populations. (Available from˜genetix/genetix/genetix.htm).Google Scholar
  3. J. Bowers, J.M. Boursiquot, P. This, K. Chu, H. Johansson and C. Meredith, Historical genetics: the parentage of Chardonnay, Gamay, and other wine grapes of Northeastern France. Science 285 (1999) 1562-1565CrossRefPubMedGoogle Scholar
  4. J.L. Brady, N.S. Scott and M.R. Thomas, DNA typing of hops (Humulus lupulus) through application of RAPD and microsatellite marker sequences converted to sequence tagged sites (STS). Euphytica 91 (1996) 277-284CrossRefGoogle Scholar
  5. J.M. Cournuet and G. Luikart, Description and power analysis of two test detecting recent population bottlenecks from allele frequency data. Genetics 144 (1996) 2001-2014Google Scholar
  6. P. Darby, New selection criteria in hop breeding. In: E. Seigner (ed.) International Hop Growersȁ9 Convention. Pulawy: Proceedings of the Scientific Commission (1999) pp. 3-6Google Scholar
  7. C. Ferris, R.A. King and G..M. Hewitt, Isolation within species and the history of glacial refugia. In: P.M. Hollingsworth, R.M. Bateman and R.J. Gornall (eds.) Molecular Systematics and Plant Evolution. London: Taylor Francis (1999) pp. 20-34Google Scholar
  8. A.M. Hadonou, R. Walden and P. Darby, Isolation and characterization of polymorphic microsatellites for assessment of genetic variation of hops (Humulus lupulus L.). Mol. Ecol. Note 4 (2004) 280-282CrossRefGoogle Scholar
  9. J. Jakše, K. Kindlhofer and B. Javornik, Assessment of genetic variation and differentiation of hop genotypes by microsatellite and AFLP markers. Genome 44 (2001) 773-782PubMedCrossRefGoogle Scholar
  10. J. Jakše, D. Bandelj and B. Javornik, Eleven new microsatellites for hop (Humulus lupulus L.). Mol. Ecol. Note 2 (2002) 544-546CrossRefGoogle Scholar
  11. R.C. Jones, D.A. Steane, B.M. Potts and R.E. Vaillancourt, Microsatellite and morphological analysis of Eucalyptus globulus populations. Can. J. For. Res. 32 (2002) 59-66CrossRefGoogle Scholar
  12. Y. Matsuoka, Y. Vigouroux, M.M. Goodman, J.G. Sanchez, E. Buckler and J. Doebley, A single domestication for maize shown by multilocus microsatellite genotyping. Proc. Natl. Acad. Sci. USA 99 (2002) 6080-6084CrossRefPubMedGoogle Scholar
  13. M. Moir, Hops – A millennium review. J. Am. Soc. Brew. Chem. 58 (2000) 131-146Google Scholar
  14. A. Murakami, Comparison of sequence of rbcL and non-coding regions of Chloroplast DNA and ITS2 region of rDNA in genus Humulus. Breed. Sci. 50 (2000) 155-160Google Scholar
  15. A. Murakami, Structural differences in the intergenic spacer of 18S-26S rDNA and molecular phylogeny using partial external transcribed spacer sequence in Hop, Humulus lupulus. Breed. Sci. 51 (2001) 163-170CrossRefGoogle Scholar
  16. A. Murakami, P. Darby, B. Javornik, M.S.S. Pais, E. Seigner, A. Lutz and P. Svoboda, Molecular evolution of hops, Humulus lupulus. In: E. Seigner (ed.) International Hop Growersȁ9 Convention. Zalec-Dobrna: Proceedings of the Scientific Commission (2003) pp. 92 ppGoogle Scholar
  17. Murakami A., Darby P., Javornik B., Pais M.S.S., Seigner E., Lutz A. and Svoboda P. 2005. Molecular Phylogeny of Hops, Humulus lupulus L. (in preparation).Google Scholar
  18. M. Nei, F. Tajima and Y. Tateno, Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19 (1983) 153-170PubMedCrossRefGoogle Scholar
  19. R.A. Neve, Hops. London: Chapman and Hall (1991).Google Scholar
  20. V. Rybáček, Hop Production. Amsterdam: Elsevier (1991).Google Scholar
  21. E.A. Small, Numerical and nomenclatural analysis of morpho-geographic taxa of Humulus. Syst. Bot. 3 (1978) 37-76CrossRefGoogle Scholar
  22. J.F. Stevens, A.W. Taylor, G.B. Nickerson, M. Ivancic, J. Henning, A. Haunold and M.L. Deinzer, Prenylflavonoid variation in Humulus lupulus: distribution and taxonomic significance of xanthogalenol and 4′-O-methylxanthohumol. Phytochemistry 53 (2000) 759-775CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Atsushi Murakami
    • 1
    • 7
    Email author
  • Peter Darby
    • 2
  • Branka Javornik
    • 3
  • Maria S. S. Pais
    • 4
  • Elisabeth Seigner
    • 5
  • Anton Lutz
    • 5
  • Petr Svoboda
    • 6
  1. 1.Research Laboratory for BrewingKirin Brewery Co., Ltd.YokohamaJapan
  2. 2.Department of Hop ResearchEast Malling ResearchKent England
  3. 3.Center for Plant Biotechnology and Breeding, Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia
  4. 4.Laboratory of Plant Biotechnology – ICATEdificio ICATCampo GrandePortugal
  5. 5.Bayer, Landesanstalt für LandwirtschaftInstitut für Pflanzenbau und PflanzenzüchtungWolnzachGermany
  6. 6.Hop Research Institute Co., Ltd.ŽatecCzech Republic
  7. 7.Kirin Brewery Co., Ltd.Shiga PrefJapan

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