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Genetic Resources and Crop Evolution

, Volume 53, Issue 8, pp 1553–1562 | Cite as

Microsatellite DNA Analysis of Wild Hops, Humulus lupulus L.

  • Atsushi MurakamiEmail author
  • Peter Darby
  • Branka Javornik
  • Maria S. S. Pais
  • Elisabeth Seigner
  • Anton Lutz
  • Petr Svoboda
Article

Abstract

To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci. There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster. Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America.

Key words

Genetic diversity Humulus lupulus Microsatellite DNA Phylogeny 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Atsushi Murakami
    • 1
    • 7
    Email author
  • Peter Darby
    • 2
  • Branka Javornik
    • 3
  • Maria S. S. Pais
    • 4
  • Elisabeth Seigner
    • 5
  • Anton Lutz
    • 5
  • Petr Svoboda
    • 6
  1. 1.Research Laboratory for BrewingKirin Brewery Co., Ltd.YokohamaJapan
  2. 2.Department of Hop ResearchEast Malling ResearchKent England
  3. 3.Center for Plant Biotechnology and Breeding, Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia
  4. 4.Laboratory of Plant Biotechnology – ICATEdificio ICATCampo GrandePortugal
  5. 5.Bayer, Landesanstalt für LandwirtschaftInstitut für Pflanzenbau und PflanzenzüchtungWolnzachGermany
  6. 6.Hop Research Institute Co., Ltd.ŽatecCzech Republic
  7. 7.Kirin Brewery Co., Ltd.Shiga PrefJapan

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