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Genetic Resources and Crop Evolution

, Volume 54, Issue 1, pp 117–128 | Cite as

RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

  • Paula Martins-Lopes
  • José Lima-Brito
  • Sónia Gomes
  • Julieta Meirinhos
  • Luís Santos
  • Henrique Guedes-PintoEmail author
Article

Abstract

Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers. Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively). The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed.

Key words

Genetic variability ISSR Molecular cultivar identification Olea europaea L. RAPD 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Paula Martins-Lopes
    • 1
  • José Lima-Brito
    • 1
  • Sónia Gomes
    • 1
  • Julieta Meirinhos
    • 1
  • Luís Santos
    • 2
  • Henrique Guedes-Pinto
    • 1
    Email author
  1. 1.Department of Genetics and Biotechnology, Centre of Genetics and BiotechnologyUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  2. 2.Department of OlivicultureNational Plant Breeding Station, INIAElvasPortugal

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