Genetic Resources and Crop Evolution

, Volume 53, Issue 5, pp 1033–1042 | Cite as

DNA Fingerprinting and Genetic Characterization of Anatolian Triticum spp. using AFLP Markers

Article
First Online:
Received:
Accepted:

Abstract

In this study, genetic analysis of Triticum spp. was carried out using AFLP markers. Six AFLP selective combinations were scored as presence and absence of bands for all the individual samples obtained from a single seed of each accession (70 accessions); T. baeoticum (21), T. monococcum (5), T. urartu (16), T. araraticum (7), T. dicoccoides (16) and T. dicoccon (5), resulting in 506 polymorphic AFLP bands. The phylogenetic tree showed two major clusters; one was composed of T. monococcum (AA) and T. baeoticum (AA), and the other cluster included T. araraticum (AAGG), T. dicoccon (AABB), T. dicoccoides (AABB), and T. urartu (AA). T. urartu, although having a diploid AA genome, did not cluster with other A genome diploids such as T. monococcum and T. baeoticum; instead it clustered together with the tetraploid species, confirming that T. urartu is the A genome progenitor. The extent of variations within and among species is discussed.

Key words

AFLP DNA fingerprinting Genetic relationship and variation Triticum spp. 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Breiman, A., Graur, D. 1995Wheat evolutionIsrael J. Plant Sci.428598Google Scholar
  2. Chapman, V., Miller, T.E., Riley, R. 1976Equivalence of the A genome of bread wheat with that of Triticum urartu Genet. Res.276976CrossRefGoogle Scholar
  3. Crow, J.F. 1986Basic Concepts in Population, Quantitativeand Evolutionary GeneticsW.H. Freeman & CoNew YorkGoogle Scholar
  4. Diamond, J. 1997Location, location, location: The first farmersScience27812431244CrossRefGoogle Scholar
  5. Dvorak, J. 1976The relationship between the genome of Triticum urartuthe A and B genomes of T. aestivum Can. J. Genet. Cytol.18371377Google Scholar
  6. Dvorak, J., McGuire, P.E., Cassidy, B. 1988Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequencesGenome30680689Google Scholar
  7. Dvorak, J., Zhang, H.B. 1990Variation in repeated nucleotide sequences sheds light on the phylogeny of the wheat B and G genomesProc. Natl. Acad. Sci. USA8796409644PubMedCrossRefGoogle Scholar
  8. Dvorak, J., di Terlizzi, P., Zhang, H.B., Resta, P. 1992The evolution of polyploid wheats: Identification of A genome donor speciesGenome362131Google Scholar
  9. Dvorak, J., Luo, M.C., Yang, Z.L., Zhang, H.B. 1998The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheatTheor. Appl. Genet.9765766CrossRefGoogle Scholar
  10. Gökgöl M. 1935. Türkiye Bugdaylari. In: Tarim Bakanligi,İstanbul Yeşilköy Tohum Islah ve Yetiştirme Enstitüsü Yayini 7 Devlet Matbaasi. Istanbul, Turkey.Google Scholar
  11. Hammer, K., Filatenko, A.A., Korzun, V. 2000Microsatellite markers – a new tool for distinguishing diploid wheat speciesGenei. Resour. Crop Evol.47497505CrossRefGoogle Scholar
  12. Hedge, S.G., Valkoun, J., Waines, J.G. 2000Genetic diversity in wild wheats and goat grassTheor. Appl. Genet.101309316CrossRefGoogle Scholar
  13. Heun, M., Schäfer-Pregl, R., Klawan, D., Castagna, R., Accerbi, M., Borghi, B., Salamini, F. 1997Site of einkorn wheat domestication identified by DNA fingerprintingScience27813121314CrossRefGoogle Scholar
  14. Incirli, A., Akkaya, M.S. 2001Assessment of genetic relationships in durum wheat cultivars using AFLP markersGenei. Resour. Crop Evol.48233238CrossRefGoogle Scholar
  15. Jaaska, V. 1993Isoenzymes in the evaluation of germplasm diversity in wild diploid relatives of cultivated wheatDamania, A.B. eds. Biodiversity and Wheat ImprovementWiley-SayceChichester, U.KGoogle Scholar
  16. Johnson, B.L. 1975Identification of the apparent B genome donor of wheatCan. J. Genet. and Cytol.172139Google Scholar
  17. Kerby, K., Kuspira, J. 1987The phylogeny of the polyploid wheats Triticum aestivum (bread wheat) and Triticum turgidum (macaroni wheat)Genome29722737Google Scholar
  18. Kihara, H. 1944Discovery of the DD-analyserone of the ancestors of Triticum vulgare Nogyo Oyobi Engei (in Japanese).191314Google Scholar
  19. Kimura, M., Crow, J.F. 1964The number of alleles that can be maintained in a finite populationGenetics49725738PubMedGoogle Scholar
  20. Le Corre, V., Bernard, M. 1995Assessment of the type and degree of restriction fragment length polymorphism (RFLP) in diploid species of the genus Triticum Theor. Appl. Genet.9010631067Google Scholar
  21. Lewontin, R.C. 1972Apportionment of human diversityEvol. Biol.6381398Google Scholar
  22. McDermott, J.M., McDonald, B.A. 1993Gene flow in plant pathosystemsAnn. Rev. Phytopathol.31353373CrossRefGoogle Scholar
  23. McFadden, E.S., Sears, E.R. 1946The origin of Triticum speltoidesits free-threshing hexaploid relativesJ. Hered.37107116Google Scholar
  24. Morrison, L.A. 1993Triticum-Aegilops systematics: taking an integrative approachDamania, A.B. eds. Biodiversity and Wheat ImprovementJohn Wiley and SonsLondon5966Google Scholar
  25. Nei, M. 1973Analysis of gene diversity in subdivided populationsProc. Natl. Acad. Sci. USA7033213323PubMedCrossRefGoogle Scholar
  26. Nei, M. 1978Estimation of average heterozygosity and genetic distance from a small number of individualsGenetics89583590PubMedGoogle Scholar
  27. Nei, M. 1987Molecular Evolutionary GeneticsColumbia Univ. PressNew York103107Google Scholar
  28. Nishikawa K. and Furuta Y. 1978. DNA content of nucleus and individual chromosomes and its evolutionary significance. In: Proc. 5th Int. Wheat Genet. Symp. pp. 133–138.Google Scholar
  29. Özkan, H., Brandolini, A., Schäfer-Pregl, R., Salamini, F. 2002AFLP analysis of a collection of tetraploid wheats indicates the origin of emmer and hard domestication in Southeast TurkeyMol. Biol. Evol.1917971801PubMedGoogle Scholar
  30. Podani, J. 1994Multivariate data analysis in ecology and systematics – a methodological guide to the SYN-TAX 5.0 packageEcol. Comp. Ser.61316SPB Academic PublishingGoogle Scholar
  31. Podani, J. 1997SYN-TAX 5.1-pc. Multivariate Data Analysis PackageSCIENTIABudapestGoogle Scholar
  32. Salamini, F., Özkan, H., Brandolini, A., Schäfer-Pregl, R., Martin, W. 2002Genetics and geography of wild cereal domestication in the near eastNature Rev.3429441Google Scholar
  33. Salina, E.A., Pestsova, E.G., Adonina, I.G., Vershinin, A.V. 1998Identification of a new family of tandem repeats in Triticeae genomesEuphytica100231237CrossRefGoogle Scholar
  34. Sarkar, P., Stebbins, G.L. 1956Morphological evidence concerning the origin of the B genome in wheatAm. J. Bot.43297304CrossRefGoogle Scholar
  35. Sourdille, P., Tavaud, M., Charmet, G., Bernard, M. 2001Transferability of wheat microsatallites to diploid Triticeae species carrying the AB and D genomesTheor. Appl. Genet.103346352CrossRefGoogle Scholar
  36. Tsunewaki, K., Takumi, S., Mori, N., Achiwa, T., Liu, Y.G. 1991Origin of polyploid wheats revealed by RFLP analysesSasakuma, T.Kinoshita, T. eds. Nuclear and Organellar Genomes of Wheat SpeciesKihara Memorial FoundationYokohamaJapan3139Google Scholar
  37. Tuna, M., Khadka, D.K., Shrestha, M.K., Arumuganathan, K., Golan-Goldhirsh, A. 2004Characterization of natural orchardgrass (Dactylis glomerata L.) populations of the Thrace Region of Turkey based on ploidy and DNA polymorphismsEuphytica1353946CrossRefGoogle Scholar
  38. Tuna, M., Vogel, K.P., Arumuganathan, K., Gill, K.S. 2001DNA content and ploidy determination of bromegrass germplasm accessions by flow cytometryCrop Sci.4116291634CrossRefGoogle Scholar
  39. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M. 1995AFLP: a new technique for DNA fingerprintingNucl. Acid Res.2344074414Google Scholar
  40. Waines, J.G., Barnhart, D. 1990Constraints to germplasm evolutionSrivastava, J.P.Damania, A.B. eds. Wheat Genetic Resources Meeting, Diverse NeedsJohn WileyChichester, UK103111Google Scholar
  41. Waines, J.G. 1995Molecular characterization of einkorn wheatPadulosi, S.Hammer, K.Heller, J. eds. Hulled Wheats, Proceedings of the First International Workshop on Hulled WheatsTuscanyItaly193198Google Scholar
  42. Yeh, F.C., Boyle, T.J.B. 1997Population genetic analysis of co-dominant and dominant markers and quantitative traitsBelg. J. Bot.129157Google Scholar
  43. Zabaeu M. and Vos P. 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application. publication no. EP 534858A1.Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Chemistry AnkaraMiddle East Technical UniversityTurkey

Personalised recommendations