Genetic Resources and Crop Evolution

, Volume 52, Issue 3, pp 245–247

Determining genetic diversity between lines of Vigna unguiculata subspecies by AFLP and SSR markers

Short Communication


AFLP (Amplified Fragment Length Polymorphisms) and SSR (Simple Sequence Repeat) markers were utilized to assess genetic diversity and relatedness between Vigna unguiculata subspecies. Three AFLP primer combinations and 10 SSR primer sets successfully identified closely related accessions, and the presence of heterogeneity in some accessions. AFLP methodology was successful in separating different species of Vigna. However, the level of intra-subspecies variation was as great as was the interspecies variation with both marker methods. The number of markers employed was insufficient to successfully group the subspecies into distinct clades.


DNA marker Germplasm Taxonomy Vigna unguiculata L. Walp. 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer 2005

Authors and Affiliations

  • A. G. GillaspieJr.
    • 1
  • M. S. Hopkins
    • 2
  • R. E. Dean
    • 3
  1. 1.U.S. Department of AgricultureAgricultural Research Service, Plant Genetic Resources Conservation UnitGriffinUSA
  2. 2.ARS, Plant Genetic Resources Conservation Unitformerly USDAGriffinUSA
  3. 3.Plant Genetic Resources Conservation UnitUniversity of GeorgiaGriffinUSA

Personalised recommendations