Genetic Resources and Crop Evolution

, Volume 52, Issue 8, pp 1111–1120

Assessing the Genetic Diversity in the International Cocoa Genebank, Trinidad (ICG,T) using Isozyme Electrophoresis and RAPD

  • O. Sounigo
  • R. Umaharan
  • Y. Christopher
  • A. Sankar
  • S. Ramdahin


Random amplified polymorphic DNA (RAPD) and isozyme electrophoresis (IE) techniques were used to estimate the level of genetic diversity in a sample of cacao germplasm existing at the International Cocoa Genebank, Trinidad. Twenty-six cocoa populations represented by 459 cocoa genotypes were analysed using IE and 22 populations represented by 353 cocoa genotypes were analysed using RAPD. Despite few differences in the classification of the populations, both techniques revealed three major groups: the indigenous trees, the cultivated Trinitario and the cultivated trees from Ecuador. Two-thirds of the partitioned diversity were found within populations and one-third between the populations, with both techniques.


Genetic diversity Isozyme Electrophoresis RAPD Theobroma cacao L. 


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Copyright information

© Springer 2005

Authors and Affiliations

  • O. Sounigo
    • 1
  • R. Umaharan
    • 2
  • Y. Christopher
    • 2
  • A. Sankar
    • 2
  • S. Ramdahin
    • 2
  1. 1.CIRADMontpellierFrance
  2. 2.Cocoa Research UnitUniversity of the West IndiesSaint AugustineTrinidad and Tobago

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