Genetic Resources and Crop Evolution

, Volume 53, Issue 1, pp 43–52

Marker-Assisted Acquisition and Core Collection Formation: A Case Study in Barley using AFLPs and Pedigree Data

  • R. van Treuren
  • I. Tchoudinova
  • L. J. M. van Soest
  • Th. J. L. van Hintum
Article

Abstract

A problem that often occurs in deciding which germplasm should be acquired to expand the diversity of a plant genetic resources collection, and which accessions should be included in a core collection, is the lack of proper data. The usefulness of an AFLP-based protocol to assist in acquisition decisions and in core collection formation was examined by using 52 barley cultivars. For validation purposes, pedigree data of the cultivars were used to calculate the ‘effective number of origin lines’ (no1), a parameter introduced in earlier research that was defined as the number of alleles per locus, not identical by descent, in a set of lines. Two AFLP primer combinations were able to distinguish all 52 cultivars from each other, and to discriminate between spring and winter crop types. Using the year of origin of the cultivars, the historical development of no1 showed a stepwise pattern, indicating the periodical release of genetically similar cultivars, alternated by the incorporation of new material. Comparison of AFLP data between cultivars and both their parents was possible in five cases. These comparisons revealed a high likelihood that the correct parents were involved but a rather skewed contribution of parents to offspring, suggesting that backcrossing had been applied. Treating the 25 cultivars that were released before 1980 and played an important role in barley cultivation as a basic collection, and the 27 more recent cultivars as potential candidates for acquisition, no1 values generated by a marker-based approach largely followed those using a random approach. Given this poor performance, a marker-based protocol to assist in acquisition decisions was not considered useful for the analysed material. If the 52 cultivars were considered to be the collection from which a core collection had to be selected, the marker-based selection showed much better results compared to a random selection. About half of the total number of origin lines could be captured with a quarter of the collection, indicating the potential utility of AFLPs in core collection formation.

Keywords

Acquisition AFLP Conservation Core collection Hordeum PGR management 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baumer, M., Cais, R. 2000Abstammung der Gerstensorten. Bayerische Anstalt für Bodenkultur und PflanzenbauFreisingGermanyGoogle Scholar
  2. Bretting, P.K., Widrlechner, M.P. 1995Genetic markers and plant genetic resource managementPlant Breed. Rev.131186Google Scholar
  3. Casas, A.M., Igartua, E., Valles, M.P., Molina-Cano, J.L. 1998Genetic diversity of barley cultivars grown in Spain, estimated by RFLP, similarity and coancestry coefficientsPlant Breed.117429435Google Scholar
  4. Ellis, R.P., McNicol, W., Baird, E., Booth, A., Lawrence, P. 1997The use of AFLPs to examine genetic relatedness in barleyMol. Breed.3359369CrossRefGoogle Scholar
  5. FAO. 1996. FAO State of the World's Plant Genetic Resources for Food and Agriculture. Food and Agriculture Organisation of the United Nations, Rome.Google Scholar
  6. Frankel, O.H., Brown, A.H.D. 1984

    Plant genetic resources today: a critical appraisal

    Holden, J.H.W.Williams, J.T. eds. Crop Genetic Resources: Conservation and Evaluation, Allen and UnwinWinchesterMassachusetts, USA
    Google Scholar
  7. Fulton, T.M., Chunwongse, J., Tanksley, S.D. 1995Microprep protocol for extraction of DNA from tomato and other herbaceous plantsPlant Mol. Biol. Rep.13207209Google Scholar
  8. Graner, A., Bjørnstad, Å., Konishi, T., Ordon, F. 2003

    Molecular diversity of the barley genome

    Bothmer, R.Sato, K.Knüpffer, H.Hintum, Th.J.L. eds. Diversity in Barley (Hordeum vulgare)ElsevierAmsterdam121142
    Google Scholar
  9. Hintum, Th.J.L., Brown, A.H.D., Spillane, C., Hodgkin, T. 2000

    Core collections of plant genetic resources

    IPGRI Technical Bulletin No. 3International Plant Genetic Resources InstituteRomeItaly
    Google Scholar
  10. Hintum, Th.J.L., Haalman, D. 1994Pedigree analysis for composing a core collection of modern cultivars, with examples from barley (Hordeum vulgare s. lat.)Theor. Appl. Genet.887074CrossRefGoogle Scholar
  11. McGregor, C.E., Treuren, R., Hoekstra, R., Hintum, Th.J.L. 2002Analysis of the wild potato germplasm of the series Acaulia with AFLPs: implications for ex situ conservationTheor. Appl. Genet.104146156CrossRefPubMedGoogle Scholar
  12. Melchinger, A.E., Graner, A., Sing, M., Messmer, M.M. 1994Relationships among European barley germplasm: I. Genetic diversity among spring and winter cultivars revealed by RFLPsCrop Sci.3411911199Google Scholar
  13. Nei, M. 1987Molecular Evolutionary GeneticsColumbia University PressNew YorkGoogle Scholar
  14. Schut, J.W., Qi, X., Stam, P. 1997Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barleyTheor. Appl. Genet.9511611168CrossRefGoogle Scholar
  15. van Soest L.J.M. 2001. Promotion of the utilisation of plant genetic resources in the Netherlands. In: S´ wiecicki W., Na-ganowska B. and Wolka B. (eds), Broad Variation and Pre-cise Characterisation – Limitation for the Future. Proceedings of the XVIth EUCARPIA Section Genetic Resources Workshop, May 16–20, 2001, Poznan, Poland, pp. 358–362.Google Scholar
  16. Treuren, R. 2001Efficiency of reduced primer selectivity and bulked DNA analysis for the rapid detection of AFLP polymorphisms in a range of crop speciesEuphytica1172737Google Scholar
  17. Treuren, R., Hintum, Th.J.L. 2001Identification of intra-accession genetic diversity in selfing crops using AFLP markers: implications for collection managementGenet. Res. Crop Evol.48287295Google Scholar
  18. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M. 1995AFLP: a new technique for DNA fingerprintingNucleic Acids Res.2344074414PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • R. van Treuren
    • 1
  • I. Tchoudinova
    • 1
  • L. J. M. van Soest
    • 1
  • Th. J. L. van Hintum
    • 1
  1. 1.Centre for Genetic Resources, The NetherlandsWageningen University and Research CentreWageningenThe Netherlands

Personalised recommendations