Genetic Resources and Crop Evolution

, Volume 52, Issue 5, pp 563–573 | Cite as

Comparative Analysis of Genetic Diversity in Two Tunisian Collections of Fig Cultivars Based on Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats Fingerprints

  • Salhi-Hannachi Amel
  • Chatti Khaled
  • Mars Messaoud
  • Marrakchi Mohamed
  • Trifi Mokhtar
Article

Abstract

This study characterized the genetic diversity of 18 Tunisian fig cultivars using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR). Both random and ISSR primers tested generated a total of 116 RAPD and 47 ISSR markers. Considerable genetic variation was observed among fig cultivars sampled from two regional Tunisian collections with an average diversity of 4.57. RAPD and ISSR banding patterns and genetic distances values reflected the high level of diversity among the collections and lower variability between the two collections. The correlation between the RAPD and ISSR similarity matrices computed for the 153 pairwise comparisons among the 18 varieties was lower and significant. An analysis of molecular variance showed that 92% of the total genetic diversity resided within collections, whereas only 8% between collections. The results indicated that in the local fig germ plasm the information provided by RAPD and ISSR is not analogous, most likely as a consequence of the fact that the two classes of markers explore, at least in part, different portions of the genome.

Keywords

Ficus carica L. Genetic diversity ISSRs Molecular variation RAPDs 

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References

  1. Beck, N.G., Lord, E.M. 1988Breeding system in Ficus carica, the common fig. I. Floral diversityAm. J. Bot.7519041912Google Scholar
  2. Bussell, J.D. 1999The distribution of random amplified polymorphic DNA (RAPD) diversity among populations of Isotoma petraea (Lobeliaceae)Mol. Ecol.8775789CrossRefGoogle Scholar
  3. Dellaporta, S.L., Wood, J., Hicks, J.B. 1983A plant DNA preparation. Version IIPlant Mol. Biol. Rep.41921Google Scholar
  4. Esselman, E.J., Jianqiangt, L.D., Crawford, J., Windus, J.L., Wolf, A.D. 1999Clonal diversity in the rare Calamagrotis proteri ssp.insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markersMol. Ecol.8443451CrossRefGoogle Scholar
  5. Excoffier, L., Smouse, P.E., Quattro, J.M. 1992Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondria DNA restriction sitesGenetics131479491PubMedGoogle Scholar
  6. Falistocco E., Antonielli L. (2002). Molecular cytogenetics of Vitis vinifera L., Ficus carica L.: location of rDNA sequences. Proceedings of the XLVI Italian Society of agricultural genetics - SIGA Annual Congress Giardini naxos, Italy, 18-21 SeptemberGoogle Scholar
  7. Felsenstein, J. 1995Phylogenetic Interference Package (PHYLIP), Version 3.5Department of Genetics, University of Washington SeattleWA, 8195, USAGoogle Scholar
  8. Fischer, M., Husi, R., Prati, D., Peintenger, M., Kleunen, M., Schmid, B. 2000RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae)Am. J. Bot.8711281137PubMedGoogle Scholar
  9. Gilbert, J.E., Lewis, R.V., Wilkinson, M.J., Caligari, P.D.S 1999Developing an appropriate strategy to assess genetic variability in plant germ plasm collectionsTheor. Appl Genet.9811251131CrossRefGoogle Scholar
  10. Guadagnuolo, R., Bianchi, D.S., Felber, F. 2001Specific genetic markers for wheat, spelt, and four relatives: comparison of isozymes, RAPDs, and wheat microsatellitesGenome44610621CrossRefPubMedGoogle Scholar
  11. Hamrick, J.L., Godt, M.J.W. 1990

    Allozyme diversity in plant species

    Brown, A.H.D.Clegg, M.T.Kahler, A.L.Weir, B.S. eds. Plant Population Genetics, Breeding, and Genetic Resources.Sinauer SunderlandMassachusetts, USA4363
    Google Scholar
  12. Hamrick, J.L., Godt, M.J.W. 1996Effect of life history traits on genetic diversity in plant speciesPhilos. Trans. R. Soc Lond. B. Biol. Sci.35112911298Google Scholar
  13. Hedfi, J., Trifi, M., Hannachi Salhi, A., OuldMohamed Salem, A., Rhouma, A., Marrakchi, M. 2001Morphological and isoenzymatic polymorphism in Tunisia fig (Ficus carica L.) collectionActa Hortic. (ISHS)605319325Google Scholar
  14. Khadari, B., Lashermes, Ph., Kjellberg, F. 1995RAPD fingerprints for identification and genetic characterization of fig (Ficus carica L.) genotypesJ. Genet. Breeding.497786Google Scholar
  15. Khadari, B., Hochu, I., Santoni, S., Oukabli, A., Ater, M., Roger, J.P., Kjellberg, F. 2001Which molecular markers are best suited to identify fig cultivars: a comparison of RAPD, ISSR and microsatellite markersActa Hortic. (ISHS)6059675Google Scholar
  16. Lai, J.A., Yang, W.C., Hsiao, J.Y. 2001An assessment of genetic relationships in cultivated teas clones and native wild tea in Taiwan using RAPD and ISSR markersBot. Bull Acad. Sinica4293100Google Scholar
  17. Lewontin, R.C. 1972The apportionment of human diversityEvol. Biol.6381398Google Scholar
  18. Mars, M., Chebli, T., Marrakchi, M. 1998Multivariate analysis of fig (Ficus carica L.) germplasm in southern TunisiaActa Hortic.4807581Google Scholar
  19. Nagaoka, T., Ogihara, O. 1997Applicability of inter-simple sequence repeat polymorphism in wheat for use as DNA markers in comparison to RFLP and RAPD markers TheorAppl. Genet.988692Google Scholar
  20. Nei, M. 1987Molecular Evolutionary GeneticsColumbia University PressNew YorkGoogle Scholar
  21. Nei, M., Li, W.H. 1979Mathematical model for studying genetical variation in terms of restriction endonucleasesProc Natl. Acad. Sci. USA7452675273Google Scholar
  22. Page, R.D.M. 1996TREEVWIEW: an application to display phylogenetic trees on personal computersComput. Appl Biosci.12357358PubMedGoogle Scholar
  23. Penner, G.A. 1996

    RAPD analysis of plant genomes

    Jauhar, P.P. eds. Methods of Genome Analysis in Plants.CRC PressBoca Raton251268
    Google Scholar
  24. Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalski, A. 1996The comparison of RFLP, RAPD, AFLP and SSR (microsatellites) markers for germplasm analysisMol. Breed.2225238CrossRefGoogle Scholar
  25. Prevost, A., Wilkinson, M.J. 1999A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivarsTheor. Appl. Genet.98107112CrossRefGoogle Scholar
  26. Raina, S.N., Rani, V., Kojima, T., Ogihara, Y., Singh, K.P., Devarumath, R.M. 2001RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hygaea) cultivars and wild speciesGenome44763772CrossRefPubMedGoogle Scholar
  27. Rhouma A. (1996). Les ressources phytogénétiques oasiennes: le figuier (Ficus carica L.). Proceedings of the 3èeeme Journées Nationales sur les Acquis de la Recherche Agronomique, Vétéeerinaire et Halieutique, Nabeul (Tunisie), p. 29Google Scholar
  28. Salhi-Hannachi A., Trifi M., Zehdi S., Hedfi J., Mars M., Rhouma A., Marrakchi M (2003). Inter-simple sequence repeat fingerprints to access genetic diversity in Tunisian fig (Ficus carica L.) germplasm Genet. Res. and Crop. Evol.(in-press)Google Scholar
  29. Sambrook, J., Fritch, E.F., Maniatis, T. 1989Molecular cloning. A laboratory manual2Cold Spring Harbor LaboratoryUnited States of Americaby ColdGoogle Scholar
  30. SAS,(1990). SAS User’s Guide: SAS/STAT, SAS BASIC Version 6.07, 4th edn. SAS Inc., Box 8000 Cary, NC 275128000Google Scholar
  31. Schneider, S., Roessli, D., Excoffier, L. 2000Arlequin: A Software for Population Genetics Data Analysis, Version 2.000Genetics and Biometry Laboratory, Department of Anthomology, University of GenevaSwitzerlandGoogle Scholar
  32. Schulter, C., Punja, Z.K. 2002Genetic diversity among natural and cultivated field populations and seed lots of American Ginseng (Panax quinquefolius L.) in CanadaInt J. Plant Sci.163427439CrossRefGoogle Scholar
  33. Sun, G.L., Diaz, O., Salomon, B., Bother, R. 1999Genetic diversity in Elymus as revealed by isozymes, RAPD and microsatellites markersGenome42420431CrossRefPubMedGoogle Scholar
  34. Trifi, M., Rhouma, A., Marrakchi, M. 2000Phylogenetic relationships in Tunisian date-palm (Phoenix dactylifera L.) germplasm collection using DNA amplification fingerprinting Agronomie20665671Google Scholar
  35. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V. 1990DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Res.1865316535PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Salhi-Hannachi Amel
    • 1
  • Chatti Khaled
    • 1
  • Mars Messaoud
    • 2
  • Marrakchi Mohamed
    • 1
  • Trifi Mokhtar
    • 1
  1. 1.Laboratoire de Geénétique Moléculaire, Immunologie and Biotechnologie, Facultée des Sciences de TunisCampus UniversitaireEl Manar TunisTunisia
  2. 2.Ecole Supérieure d’Horticulture et d’ElevageSousseTunisia

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