Genetic evidence for speciation in Cannabis (Cannabaceae)
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Abstract
Sample populations of 157 Cannabis accessions of diverse geographic origin were surveyed for allozyme variation at 17 gene loci. The frequencies of 52 alleles were subjected to principal components analysis. A scatter plot revealed two major groups of accessions. The sativa gene pool includes fiber/seed landraces from Europe, Asia Minor, and Central Asia, and ruderal populations from Eastern Europe. The indica gene pool includes fiber/seed landraces from eastern Asia, narrow-leafleted drug strains from southern Asia, Africa, and Latin America, wide-leafleted drug strains from Afghanistan and Pakistan, and feral populations from India and Nepal. A third putative gene pool includes ruderal populations from Central Asia. None of the previous taxonomic concepts that were tested adequately circumscribe the sativa and indica gene pools. A polytypic concept of Cannabis is proposed, which recognizes three species, C. sativa, C. indica and C. ruderalis, and seven putative taxa.
Keywords
Allozyme Cannabis Evolution Genetics Origin TaxonomyAbbreviations
- PCA
– principal components analysis
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References
- Anderson, L.C. 1974A study of systematic wood anatomy in CannabisHarvard Univ. Bot. Mus. Leafl.242936Google Scholar
- Anderson, L.C. 1980Leaf variation among Cannabis species from a controlled gardenHarvard Univ. Bot. Mus. Leafl.286169Google Scholar
- Anonymous (1989) The Seed Bank Catalogue. Ooy, The Netherlands. [authorship attributed to N. Schoenmakers.]Google Scholar
- Bócsa, I. 1999Genetic improvement: conventional approachesRanalli, P. eds. Advances in Hemp ResearchHaworth PressBinghamton NY153184Google Scholar
- Bócsa, I., Karus, M. 1998The Cultivation of HempHemptechSebastopol, CAGoogle Scholar
- Bredemann, G., Schwanitz, Fr., Sengbusch, R. 1956Problems of modern hemp breeding, with particular reference to the breeding of varieties with little or no hashishBull. Narc.83135Google Scholar
- de Candolle A. 1885. Hemp – Cannabis sativa L. In: Origin of Cultivated Plants, D. Appleton, New York, pp. 148–149Google Scholar
- Cherniak, L. 1982The Great Books of Cannabis, vol. I, Book IICherniak/Damele PublishingOakland, CAGoogle Scholar
- Chopra, I.C., Chopra, R.N. 1957The use of Cannabis drugs in IndiaBull. Narc.9429Google Scholar
- Clarke R.C. 1995. Hemp (Cannabis sativa L.) cultivation in the Tai’an district of Shandong Province, Peoples Republic of China. J. Int. Hemp Assoc. 2: 57, 60–65Google Scholar
- Crawford, D.J. 1989Enzyme electrophoresis and plant systematicsSoltis, D.E.Soltis, P.S. eds. Isozymes in Plant BiologyDioscorides PressPortland OR146164Google Scholar
- Davidyan, G.G. 1972Hemp: biology and initial material for breedingTr. Prikl. Bot. Genet. Sel.481160(in Russian)Google Scholar
- Delile, R.-A. 1849Index seminum horti botanici MonspeliensisAnn. Sci. Nat. Bot. Biol. Veg.12365366Google Scholar
- Dewey L.H. 1914. Hemp. In: USDA Yearbook 1913. United States Department of Agriculture, Washington, DC, pp. 283–347Google Scholar
- Doebley, J. 1989Isozymic evidence and the evolution of crop plantsSoltis, D.E.Soltis, P.S. eds. Isozymes in Plant BiologyDioscorides PressPortland, OR165191Google Scholar
- Du Toit, B.M. 1980Cannabis in AfricaA.A. BalkemaRotterdam, The NetherlandsGoogle Scholar
- Emboden, W.A. 1974Cannabis – a polytypic genusEcon. Bot.28304310Google Scholar
- Emboden, W.A. 1981The genus Cannabis and the correct use of taxonomic categoriesJ. Psychoactive Drugs131521Google Scholar
- Faeti, V., Mandolino, G., Ranalli, P. 1996Genetic diversity of Cannabis sativa germplasm based on RAPD markersPlant Breed.115367370Google Scholar
- Fleming, M.P., Clarke, R.C. 1998Physical evidence for the antiquity of Cannabis sativa L.J. Int. Hemp Assoc.58093Google Scholar
- Hamrick, J.L. 1989Isozymes and the analysis of genetic structure in plant populationsSoltis, D.E.Soltis, P.S. eds. Isozymes in Plant BiologyDioscorides PressPortland, OR87105Google Scholar
- Hillig, K.W., Iezzoni, A.F. 1988Multivariate analysis of a sour cherry germplasm collectionJ. Am. Soc. Hort. Sci.113928934Google Scholar
- Jagadish, V., Robertson, J., Gibbs, A. 1996RAPD analysis distinguishes Cannabis sativa samples from different sourcesForensic Sci. Int.79113121Google Scholar
- Janischevsky D.E. 1924. Forma konopli na sornykh mestakh v Yugo-vostochnoi Rossii. In: Chiuevsky I.A. (ed.), Uchen. Zap. Gosud. Saratovsk. Chernyshevskogo Univ., 2(2): 3–17, Saratov University Press, Saratov, USSRGoogle Scholar
- Kephart, S.R. 1990Starch gel electrophoresis of plant isozymes: a comparative analysis of techniquesAm. J. Bot.77316368Google Scholar
- de Lamarck J.B. 1785. Encyclopédique Méthodique de Botanique, vol. 1, Pt. 2. Paris, France, pp. 694–695Google Scholar
- Lawi-Berger, C., Miége, M.N., Kapétanidis, I., Miége, J. 1982Contribution a l’etude chimiotaxonomique de Cannabis sativa LCR Acad. Sci. Paris295397402Google Scholar
- Lemeshev, N., Rumyantseva, L., Clarke, R.C. 1994Maintenance of Cannabis germplasm in the Vavilov Research Institute gene bank – 1993J. Int. Hemp Assoc.135Google Scholar
- Li, H.-L. 1974An archaeological and historical account of Cannabis in ChinaEcon. Bot.28437448Google Scholar
- Mandolino, G., Ranalli, P. 2002The applications of molecular markers in genetics and breeding of hempJ. Indust. Hemp.7723Google Scholar
- Meijer, E.P.M. 1994Diversity in CannabisWageningen Agricultural UniversityWageningen, The NetherlandsDoctoral thesisGoogle Scholar
- Meijer, E.P.M. 1995Fibre hemp cultivars: a survey of origin, ancestry, availability and brief agronomic characteristicsJ. Int. Hemp Assoc.26673Google Scholar
- Meijer, E.P.M. 1999Cannabis germplasm resourcesRanalli, P. eds. Advances in Hemp ResearchHaworth PressBinghamton, NY133151Google Scholar
- Meijer, E.P.M., Keizer, L.C.P. 1996Patterns of diversity in CannabisGenet. Resour. Crop Evol.434152Google Scholar
- Meijer, E.P.M., Soest, L.J.M. 1992The CPRO Cannabis germplasm collectionEuphytica62201211Google Scholar
- Migal N.D. 1991. Genetics of polymorphic sex evolution in hemp. Genetika 27: 1561–1569. (Translated in Soviet Genetics, March 1992: 1095–1102)Google Scholar
- Morden C.W., Doebley J. and Schertz K.F. 1987. A Manual of Techniques for Starch Gel Electrophoresis of Sorghum Isozymes. Texas Agric. Exp. Sta. Misc. Publ. 1635, College Station, TXGoogle Scholar
- Nei, M. 1987Molecular Evolutionary GeneticsColumbia University PressNew YorkGoogle Scholar
- Pickersgill, B. 1988The genus Capsicum: A multidisciplinary approach to the taxonomy of cultivated and wild plantsBiol. Zentralbl.107381389Google Scholar
- SAS Institute2002JMP Statistics and Graphics GuideSAS InstituteCary, NCGoogle Scholar
- Schultes, R.E. 1970Random thoughts and queries on the botany of CannabisJoyce, C.R.B.Curry, S.H. eds. The Botany and Chemistry of CannabisJ. and A. ChurchillLondon1138Google Scholar
- Schultes R.E. 1973. Man and marijuana. Nat. Hist. 82: 58–63, 80, 82Google Scholar
- Schultes, R.E., Klein, W.M., Plowman, T., Lockwood, T.E. 1974Cannabis: an example of taxonomic neglectHarvard Univ. Bot. Mus. Leafl.23337367Google Scholar
- Serebriakova, T.Ya., Sizov, I.A. 1940Cannabinaceae LindlVavilov, N.I. eds. Kulturnaja Flora SSSR, vol. 5.Moscow-LeningradUSSR153Google Scholar
- Shields, C.R., Orton, T.J., Stuber, C.W. 1983An outline of general resource needs and procedures for the electrophoretic separation of active enzymes from plant tissueTanksley, S.D.Orton, T.J. eds. Isozymes in Plant Genetics and Breeding. Part AElsevier Science PublishersAmsterdam443516Google Scholar
- Siniscalco Gigliano, G. 2001Cannabis sativa L. – Botanical problems and molecular approaches in forensic investigationsForensic Sci. Rev.13217Google Scholar
- Small, E. 1979The Species Problem in Cannabis, vol. 1Corpus Information ServicesTorontoGoogle Scholar
- Small, E., Beckstead, H.D. 1973Common cannabinoid phenotypes in 350 stocks of CannabisLloydia36144165Google Scholar
- Small, E., Cronquist, A. 1976A practical and natural taxonomy for CannabisTaxon25405435Google Scholar
- Small, E., Jui, P.Y., Lefkovitch, L.P. 1976A numerical taxonomic analysis of Cannabis with special reference to species delimitationSyst. Bot.16784Google Scholar
- Soltis, D.E., Haufler, C.H., Darrow, D.C., Gastony, G.J. 1983Starch gel electrophoresis of ferns: A compilation of grinding buffers, gel and electrode buffers, and staining schedulesAm. Fern J.73927Google Scholar
- Vavilov N.I. 1926. The origin of the cultivation of ‘primary’ crops, in particular cultivated hemp. In: Studies on the Origin of Cultivated Plants, Institute of Applied Botany and Plant Breeding, Leningrad, USSR, pp. 221–233Google Scholar
- Vavilov N.I. and Bukinich D.D. 1929. Konopli. Zemledel’cheskii Afghanistan. Tr. Prikl. Bot. Genet. Sel. 33(Suppl.): 380–382, 474, 480, 584–585, 604 Reissued in 1959 by Izdatel’stuo Akademii Nauk SSSR, Moskva- LeningradGoogle Scholar
- Weeden, N.F., Wendel, J.F. 1989Genetics of plant isozymesSoltis, D.E.Soltis, P.S. eds. Isozymes in Plant BiologyDioscorides PressPortland, OR4672Google Scholar
- Wendel, J.F., Weeden, N.F. 1989Visualization and interpretation of plant isozymesSoltis, D.E.Soltis, P.S. eds. Isozymes in Plant BiologyDioscorides PressPortland, OR545Google Scholar
- Wiley, E.O. 1981PhylogeneticsJohn Wiley and SonsNew YorkGoogle Scholar
- Zeven A.C. and Zhukovsky P.M. 1975. Cannabidaceae [sic]. In: Dictionary of Cultivated Plants and their Centres of Diversity, Centre for Agricultural Publishing and Documentation, Wageningen, The Netherlands, pp. 62–63, 129–130Google Scholar
- Zhukovsky P.M. 1964. Hemp (Cannabis L.) In: Cultivated Plants and their Wild Relatives, 2nd edn., Ch. 9. Publishing House Molos, Leningrad, USSR, pp. 456–458Google Scholar