Lectins and ELLSA as powerful tools for glycoconjugate recognition analyses
Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. (iii) Applications of lectins for the detection and identification of N-glycan and/or Tn glycotope in glycoconjugates were intergraded. (iv) The polyvalency of the glycotopes in glycans was found to play a critical role in glycan–lectin recognition. This is an unexplored area of glycobiology and one of the most promising directions toward the coming glycoscience transformation.
KeywordsApplied lectins Glycotopes (epitopes) Glycan recognition Lectin–glycan interactions Polyvalency of glycotopes
Quantitative precipitin assay
Quantitative inhibition assay
Hemagglutination inhibition assay
Enzyme-linked lectinosorbent assay
Enzyme-linked inhibition assay
- Mass RP
Mass relative potency
Molecular mass cutoff
The authors would like to thank Drs. W.J. Peumans and E.J. Van Damme for our long-term collaboration in obtaining many of the promising data for this review.
This work was supported by a CGU research grant, BMRP 008 and MICCs Forever Fund.
Kwei-San, Tao-yuan, Taiwan.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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