Glycoconjugate Journal

, Volume 34, Issue 3, pp 421–425 | Cite as

MS/IR, a new MS-based hyphenated method for analysis of hexuronic acid epimers in glycosaminoglycans

  • Baptiste Schindler
  • Gina Renois-Predelus
  • Nassiba Bagdadi
  • Sihem Melizi
  • Loïc Barnes
  • Stéphane Chambert
  • Abdul-Rahman Allouche
  • Isabelle Compagnon
Original Article

Abstract

We report an original MS-based hyphenated method for the elucidation of the epimerization in GAG fragments. It consists of measuring simultaneously the MS/MS spectrum and the gas phase IR spectrum to gain direct structural information. This is possible using a customized MS instrument, modified to allow injection of a tunable IR laser inside of the instrument for in situ spectroscopy of trapped ions. The proof of principle of this approach is performed in the case of a hyaluronic acid tetrasaccharide standard. In addition, we provide the reference IR fingerprint of glucuronic and Iduronic monosaccharide standards. Remarkably, we show that the gas phase IR fingerprint of reference hexuronic acid monosaccharides proves to be transposable to oligosaccharides. Therefore, the method presented here is predictive and allows structural elucidation of unknown GAG fragments, even in the absence of referenced standards.

Keywords

GAG fragments Mass spectrometry gas phase IR spectroscopy hexuronic acid epimers 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institut Lumière MatièreUniv Lyon, Université Claude Bernard Lyon 1, CNRSVilleurbanneFrance
  2. 2.Univ Lyon, INSA-Lyon, CNRS, Université Lyon 1, CPE Lyon, ICBMSUMR 5246, Bâtiment Jules VerneVilleurbanneFrance
  3. 3.Institut Universitaire de France IUFParisFrance

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