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Glycoconjugate Journal

, Volume 33, Issue 2, pp 147–157 | Cite as

Structural characterization and biological activities of two α-glucans from Radix Paeoniae Alba

  • Wuxia Zhang
  • Peng Li
  • Dan Song
  • Hong Niu
  • Songshan Shi
  • Shunchun WangEmail author
  • Jinyou DuanEmail author
Original Article

Abstract

Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, cancer, and other diseases. In this study, two polysaccharides RPAPW1 and RPAPW2 were isolated from Radix Paeoniae Alba by DEAE-52 cellulose chromatography and G-25 sephadex. According to physicochemical methods, NMR and methylation analysis, RPAPW1 and RPAPW2 were established to be α-glucans consisting of predominant 4-linked α- Glc residues branched at O-6 and contained trace amount of protein and uronic acid. Immunological tests indicated that RPAPW1, RPAPW2 and could promote splenocyte proliferation and RAW264.7 phagocytic activity. In vitro, RPAPW1 and RPAPW2 elicited a week reducing power, DPPH scavenging activity and could not protect the PC12 cells from H2O2 damage. These data implied polysaccharides RPAPW1 and RPAPW2 had the potential to be a natural immunopotentiating and antioxidant supplement for preparing functional foods and nutraceuticals.

Keywords

Radix Paeoniae Alba Polysaccharides Antioxidant activity Immunological activity 

Notes

Acknowledgments

This work was supported by Program for New Century Excellent Talents in University (NCET-13-0480) and the National Natural Science Foundation of China (NSFC) [Grant 31270860].

Compliance with ethical standards

Conflict of interest

We declare that we have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.College of scienceNorthwest A&F UniversityYanglingChina
  2. 2.College of life scienceNorthwest A&F UniversityYanglingChina
  3. 3.Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina

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