Glycoconjugate Journal

, Volume 29, Issue 5–6, pp 365–377 | Cite as

Structure of a β-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-κB signaling

  • Jianping Fang
  • Ying Wang
  • Xiaofen Lv
  • Xiaokun Shen
  • Xinyan Ni
  • Kan DingEmail author


A soluble homogeneous β-glucan, GFPBW1, with a molecular mass of 300 kDa was purified from the fraction of the fruit bodies of Grifola frondosa extracted with 5 % NaOH. Using various methods, such as infrared spectroscopy, NMR, methylation and monosaccharide composition analysis, its structure was determined to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue. It induced TNF-α and IL-6 production and the activation of Syk and NF-κB signaling in resident peritoneal macrophages from ICR mice, which could be significantly inhibited by the blocking reagent laminarin. A competitive phagocytosis assay with FITC-zymosan indicated that GFPBW1 could bind to DC-associated C-type lectin 1 (Dectin-1). The TNF-α secretion and activation of Syk/NF-κB signaling triggered by GFPBW1 were enhanced in RAW264.7 cells overexpressing wild but not mutant (Δ38 and Y15S) Dectin-1. Furthermore, GFPBW1 potentiated the Concanavalin A-induced proliferative response of splenocytes and inhibited Sarcoma-180 growth allografted in ICR mice but not in immunodeficient BALB/c nu/nu mice. These results suggested that the antitumor activity of GFPBW1 was partially associated with the activation of macrophages via the Dectin-1/Syk/NF-κB signaling pathway. This molecule could be a promising biological response modifier with clear application for antitumor therapies.


β-glucan Grifola frondosa Biological response modifier Dectin-1 



Biological response modifiers


Caspase recruitment domain 9


Concanavalin A


Complement receptor 3




Dendritic cells


DC-associated C-type lectin 1


Dimethyl sulfoxide


Fluorescence-activated cell sorting


Gas chromatography

G. frondosa

Grifola frondosa (Fr.) S. F. Gray




High performance gel permeation chromatography


Horseradish peroxidase


Heteronuclear single quantum coherence




Immunoreceptor tyrosine-based activation motif




Mass spectrometry


Nuclear factor κB


Nuclear magnetic resonance


Polymerase chain reaction


Polymyxin B




Pattern recognition receptors


Spleen tyrosine kinase


Trifluoroacetic acid


Toll like receptor 2


Sodium dodecyl sulfate



This work was supported by New Drug Creation and Manufacturing Program (2012ZX09301001-003), National Science Fund for Distinguished Young Scholars (81125025) and the funds for Industry-University-Research Institution Alliance in Guangdong Province, China (2010A090200041).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jianping Fang
    • 1
  • Ying Wang
    • 1
  • Xiaofen Lv
    • 1
  • Xiaokun Shen
    • 1
  • Xinyan Ni
    • 1
  • Kan Ding
    • 1
    Email author
  1. 1.Glycochemistry & Glycobiology Lab, Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina

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