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Glycoconjugate Journal

, Volume 29, Issue 7, pp 457–465 | Cite as

Characterization and cloning of GNA-like lectin from the mushroom Marasmius oreades

  • Michiko Shimokawa
  • Ayako Fukudome
  • Ryoko Yamashita
  • Yuji Minami
  • Fumio YagiEmail author
  • Hiroaki Tateno
  • Jun Hirabayashi
Article

Abstract

A new mannose-recognizing lectin (MOL) was purified on an asialofetuin-column from fruiting bodies of Marasmius oreades grown in Japan. The lectin (MOA) from the fruiting bodies of the same fungi is well known to be a ribosome-inactivating type lectin that recognizes blood-group B sugar. However, in our preliminary investigation, MOA was not found in Japanese fruiting bodies of M. oreades, and instead, MOL was isolated. Gel filtration showed MOL is a homodimer noncovalently associated with two subunits of 13 kDa. The N-terminal sequence of MOL was blocked. The sequence of MOL was determined by cloning from cDNA and by protein sequencing of enzyme-digested peptides. The sequence shows mannose-binding motifs of bulb-type mannose-binding lectins from plants, and similarity to the sequences. Analyses of sugar-binding specificity by hemagglutination inhibition revealed the preference of MOL toward mannose and thyroglobulin, but asialofetuin was the strongest inhibitor of glycoproteins tested. Furthermore, glycan-array analysis showed that the specificity pattern of MOL was different from those of typical mannose-specific lectins. MOL preferred complex–type N-glycans rather than high-mannose N-glycans.

Keyword

Fungus GNA-like Lectin Mannose-binding Marasmius oreades 

Abbreviations

GNA

Galanthus nivalis agglutinin

LDL

Lyophyllum descades lectin

MOA

Marasmius oreades agglutinin

MOL

Marasmius oreades lectin

RVL

Remusatia vivipara lectin

TxcL1

Tulipa hybrid lectin 1 with complex specificity

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Michiko Shimokawa
    • 1
  • Ayako Fukudome
    • 2
  • Ryoko Yamashita
    • 2
  • Yuji Minami
    • 2
  • Fumio Yagi
    • 2
    Email author
  • Hiroaki Tateno
    • 3
  • Jun Hirabayashi
    • 3
  1. 1.Department of Applied Biological Chemistry, The United Graduate School of Agricultural SciencesKagoshima UniversityKagoshimaJapan
  2. 2.Department of Biochemical Science and Technology, Faculty of AgricultureKagoshima UniversityKagoshimaJapan
  3. 3.Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and TechnologyIbarakiJapan

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