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Glycoconjugate Journal

, Volume 27, Issue 2, pp 259–265 | Cite as

A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica

  • Shuang Zhao
  • Yongchang Zhao
  • Shuhong Li
  • Jingkun Zhao
  • Guoqing Zhang
  • Hexiang WangEmail author
  • Tzi Bun NgEmail author
Article

Abstract

A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from fresh fruiting bodies of the wild mushroom Russula delica. The lectin was composed of two identical subunits, each with a molecular weight of 30 kDa. It was adsorbed on both SP-Sepharose and Q-Sepharose and unadsorbed on DEAE-cellulose. Its hemagglutinating activity was stable up to 70°C, and in HCl and NaOH solutions of concentrations up to 25 and 12.5 mM, respectively. The activity was inhibited by inulin and o-nitrophenyl-β-D-galactopyranoside. Al3+, Fe3+ and Zn2+ ions, but not by Ca2+, Mg2+ and Mn2+ ions. Mg2+ ions at 10 mM concentration potentiated the hemagglutinating activity of the lectin. Russula delica lectin was devoid of mitogenic activity toward mouse splenocytes, but potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.88 µM and 0.52 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.26 μM.

Keywords

Lectin Wild mushroom Russula delica Antiproliferative Purification 

Notes

Acknowledgments

This work was financially supported by National Grants of China (nyhyzx07-008 and 2006BAD07A01).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shuang Zhao
    • 1
    • 2
  • Yongchang Zhao
    • 3
  • Shuhong Li
    • 3
  • Jingkun Zhao
    • 1
  • Guoqing Zhang
    • 1
  • Hexiang Wang
    • 1
    Email author
  • Tzi Bun Ng
    • 4
    Email author
  1. 1.State Key Laboratory for Agrobiotechnology and Department of MicrobiologyChina Agricultural UniversityBeijingChina
  2. 2.Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijingChina
  3. 3.Institute of Biotechnology and Germplasmic ResourceYunnan Academy of Agricultural ScienceKunmingChina
  4. 4.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong KongNew TerritoriesChina

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