Glycoconjugate Journal

, Volume 27, Issue 2, pp 259–265 | Cite as

A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica

  • Shuang Zhao
  • Yongchang Zhao
  • Shuhong Li
  • Jingkun Zhao
  • Guoqing Zhang
  • Hexiang WangEmail author
  • Tzi Bun NgEmail author


A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from fresh fruiting bodies of the wild mushroom Russula delica. The lectin was composed of two identical subunits, each with a molecular weight of 30 kDa. It was adsorbed on both SP-Sepharose and Q-Sepharose and unadsorbed on DEAE-cellulose. Its hemagglutinating activity was stable up to 70°C, and in HCl and NaOH solutions of concentrations up to 25 and 12.5 mM, respectively. The activity was inhibited by inulin and o-nitrophenyl-β-D-galactopyranoside. Al3+, Fe3+ and Zn2+ ions, but not by Ca2+, Mg2+ and Mn2+ ions. Mg2+ ions at 10 mM concentration potentiated the hemagglutinating activity of the lectin. Russula delica lectin was devoid of mitogenic activity toward mouse splenocytes, but potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.88 µM and 0.52 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.26 μM.


Lectin Wild mushroom Russula delica Antiproliferative Purification 



This work was financially supported by National Grants of China (nyhyzx07-008 and 2006BAD07A01).


  1. 1.
    Guillot, J., Konska, G.: Lectins in higher fungi. Biochem. Syst. Ecol. 25, 203–230 (1997)CrossRefGoogle Scholar
  2. 2.
    Ng, T.B.: Peptides and proteins from fungi. Peptides 25, 1055–1073 (2004)CrossRefPubMedGoogle Scholar
  3. 3.
    Wang, H.X., Ng, T.B., Ooi, V.E.C.: Lectins from mushrooms. Mycol. Res. 102, 897–906 (1998)CrossRefGoogle Scholar
  4. 4.
    Wang, H.X., Liu, W.K., Ng, T.B.: The immunomodulatory and antitumor activities of lectins from mushroom Tricholoma mongolicum. Immunopharmacology 31, 205–211 (1996)CrossRefPubMedGoogle Scholar
  5. 5.
    Yu, L.G., Fernig, D.J., Smith, J.A., Milton, J.D., Rhodes, J.M.: Reversible inhibition of proliferation of epithelial cell lines by Agaricus bisporus (edible mushroom) lectin. Cancer Res. 53, 4627–4632 (1993)PubMedGoogle Scholar
  6. 6.
    Yagi, F., Miyamoto, M., Abe, T., Minami, T., Tadera, K., Goldstein, I.J.: Purification and carbohydrate-binding specificity of Agrocybe cylindracea lectin. Glycoconj. J. 14, 281–288 (1997)CrossRefPubMedGoogle Scholar
  7. 7.
    Wang, H.X., Ng, T.B., Liu, Q.H.: Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sci. 70, 877–886 (2002)CrossRefPubMedGoogle Scholar
  8. 8.
    Ko, J.L., Hsu, C.I., Lin, R.H., Kao, C.L., Lin, J.Y.: A new fungal immunomodulatory protein, FIP-fve isolated from the edible mushroom. Flammulina velutipes and its complete amino acid sequence. Eur. J. Biochem. 228, 244–249 (1995)CrossRefPubMedGoogle Scholar
  9. 9.
    Kawagishi, H., Mitsunaga, S.I., Yamawaki, M., Ido, M., Shimada, A., Kinoshita, T., Murata, T., Usui, T., Kimura, A., Chiba, S.: A lectin from mycelia of the fungus Ganoderma lucidum. Phytochemistry 44, 7–10 (1997)CrossRefPubMedGoogle Scholar
  10. 10.
    Kawagishi, H., Nomura, A., Mizuno, T., Kimura, A., Chiba, S.: Isolation and characterization of a lectin from Grifola frondosa fruiting bodies. Biochim. Biophys. Acta 1034, 247–252 (1990)PubMedGoogle Scholar
  11. 11.
    Lin, J.Y., Chou, T.B.: Isolation and characterization of a lectin from edible mushroom Volvariella volvacea. J. Biochem. 96, 35–40 (1984)PubMedGoogle Scholar
  12. 12.
    She, Q.B., Ng, T.B., Liu, W.K.: A novel lectin with potent immunomodulatory activity isolated from both fruiting bodies and cultured mycelia of the edible mushroom Volvariella volvacea. Biochem. Biophys. Res. Commun. 247, 106–111 (1998)CrossRefPubMedGoogle Scholar
  13. 13.
    Han, C.H., Liu, Q.H., Ng, T.B., Wang, H.X.: A novel homodimeric lactose-binding lectin from the edible split gill medicinal mushroom Schizophyllum commune. Biochem. Biophys. Res. Commun. 336, 252–257 (2005)CrossRefPubMedGoogle Scholar
  14. 14.
    Laemmli, U.K., Favre, M.: Maturation of the head of bacteriophage T4. J. Mol. Biol. 80, 575–599 (1973)CrossRefPubMedGoogle Scholar
  15. 15.
    Liu, Q.H., Wang, H.X., Ng, T.B.: Isolation and characterization of a novel lectin from the wild mushroom Xerocomus spadiceus. Peptides 25, 7–10 (2004)CrossRefPubMedGoogle Scholar
  16. 16.
    Wang, H.X., Gao, J., Ng, T.B.: A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem. Biophys. Res. Commun. 275, 810–816 (2000)CrossRefPubMedGoogle Scholar
  17. 17.
    Wang, H.X., Ng, T.B., Liu, W.K., Ooi, V.E.C., Chang, S.T.: Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum. Int. J. Pept. Protein Res. 46, 508–513 (1995)PubMedCrossRefGoogle Scholar
  18. 18.
    Feng, K., Liu, Q.H., Ng, T.B., Liu, H.Z., Li, J.Q., Chen, G., Sheng, H.Y., Xie, Z.L., Wang, H.X.: Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens. Biochem. Biophys. Res. Commun. 345, 1573–1578 (2006)CrossRefPubMedGoogle Scholar
  19. 19.
    Cooper, D.N., Boulianne, R.P., Charlton, S., Fanell, E.M., Sucher, A., Lum, B.C.: Fungal galectins, sequence and specificity of two isolectins from Coprinus cinereus. J. Biol. Chem. 272, 1514–1521 (1997)CrossRefPubMedGoogle Scholar
  20. 20.
    Matsumoto, H., Natsume, A., Ueda, H., Saitoh, T., Ogawa, H.: Screening of a unique lectin from 16 cultivable mushrooms with hydrid glycoprotein and neoproteoglycan probes and purification of a novel N-acetylglucosamine-specific lectin from Oudemansiella platyphylla fruiting body. Biochim. Biophys. Acta 1526, 37–43 (2001)PubMedGoogle Scholar
  21. 21.
    Kawagishi, H., Mori, H., Uno, A., Kimura, A., Chiba, S.: A sialic acid binding lectin from the mushroom Hericium erinaceum. FEBS Lett. 340, 56–58 (1994)CrossRefPubMedGoogle Scholar
  22. 22.
    Bottcher, M.: Grosse, F.: HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction. Nucleic Acids Res. 25, 1709–1714 (1997)CrossRefPubMedGoogle Scholar
  23. 23.
    Jung, E.C., Kim, K.D., Kim, H.H.: A mushroom lectin from ascomycete Cordyceps militaris. Biochim. Biophys. Acta 1770, 833–838 (2007)PubMedGoogle Scholar
  24. 24.
    Liu, Q.H., Wang, H.X., Ng, T.B.: First report of a xylose-specific lectin with potent hemagglutinating, antiproliferative and anti-mitogenic activities from a wild ascomycete mushroom. Biochim. Biophys. Acta 1760, 1914–1919 (2006)PubMedGoogle Scholar
  25. 25.
    Patrick, H.K., Ng, T.B.: A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells. Biochem. Biophys. Res. Commun. 314, 988–993 (2004)CrossRefGoogle Scholar
  26. 26.
    Dong, Y.C., Feng, S.S.: Poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles prepared by high pressure homogenization for paclitaxel chemotherapy. Int. J Pharm 342, 208–214 (2007)CrossRefPubMedGoogle Scholar
  27. 27.
    Wong, J.H., Wang, H.X., Ng, T.B.: A haemagglutinin from the medicinal fungus Cordyceps militaris. Biosci. Rep. 29, 321–327 (2009)CrossRefPubMedGoogle Scholar
  28. 28.
    Zhang, G.Q., Sun, J., Wang, H.X., Ng, T.B.: A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa. Acta Biochim. Pol. 56, 415–421 (2009)PubMedGoogle Scholar
  29. 29.
    Zhao, J.K., Wang, H.X.: Ng, T.B.:Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella. Toxicon. 53, 360–366 (2009)CrossRefPubMedGoogle Scholar
  30. 30.
    Zheng, S.Y., Li, C.X., Ng, T.B., Wang, H.X.: A lectin with mitogenic activity from the edible wild mushroom Boletus edulis. Process Biochem. 42, 1620–1624 (2007)CrossRefGoogle Scholar
  31. 31.
    Li, Y.R., Liu, Q.H., Wang, H.X., Ng, T.B.: A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. Biochim. Biophys. Acta 1780, 51–57 (2008)PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shuang Zhao
    • 1
    • 2
  • Yongchang Zhao
    • 3
  • Shuhong Li
    • 3
  • Jingkun Zhao
    • 1
  • Guoqing Zhang
    • 1
  • Hexiang Wang
    • 1
    Email author
  • Tzi Bun Ng
    • 4
    Email author
  1. 1.State Key Laboratory for Agrobiotechnology and Department of MicrobiologyChina Agricultural UniversityBeijingChina
  2. 2.Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijingChina
  3. 3.Institute of Biotechnology and Germplasmic ResourceYunnan Academy of Agricultural ScienceKunmingChina
  4. 4.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong KongNew TerritoriesChina

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