Glycoconjugate Journal

, 26:1171

Norwalk virus-like particles bind specifically to A, H and difucosylated Lewis but not to B histo-blood group active glycosphingolipids

  • Jonas Nilsson
  • Gustaf E. Rydell
  • Jacques Le Pendu
  • Göran Larson
Article

DOI: 10.1007/s10719-009-9237-x

Cite this article as:
Nilsson, J., Rydell, G.E., Le Pendu, J. et al. Glycoconj J (2009) 26: 1171. doi:10.1007/s10719-009-9237-x

Abstract

Noroviruses and norovirus virus-like particles (VLPs) exhibit strain specific patterns in their binding to ABH and Lewis histo-blood group antigens. In this study we demonstrate for the first time specific binding of Norwalk virus VLPs to type 1 and type 2 chain glycosphingolipids (GSLs) carrying ABH and Lewis antigens. N-succinimidyl-3-tributylstannyl benzoate (ATE) was precursor labeled with 125I and then conjugated to VLPs. The 125I-VLPs were used in GSL thin-layer chromatogram binding assays and displayed binding to H type 1, Lewis b, A type 1, A Lewis b GSLs but no binding to B type 1 or B Lewis b GSLs. For the type 2 chain GSLs the Norwalk VLPs bound to H type 2, Lewis y, A type 2 and A Lewis y. In addition, the VLPs bound to several complex GSLs from blood group O and A, but not from blood group B red blood cells.

Keywords

Norovirus Virus-like particle Glycosphingolipid ABH antigen Secretor 

Abbreviations

ATE

N-succinimidyl-3-tributylstannyl benzoate

BSA

bovine serum albumin

CBA

chromatogram binding assay

Cer

ceramide

cpm

counts per minute

DMSO

dimethylsulfoxide

ELISA

enzyme-linked immunosorbent assay

Fuc

fucose

FUT

fucosyltransferase gene

G

genogroup

Gal

galactose

GalNAc

N-acetylgalactosamine

Glc

glucose

GlcNAc

N-acetylglucosamine

GSL

glycosphingolipid

HPLC

high performance liquid chromatography

HRP

horseradish peroxidase

Le

Lewis

NV

Norwalk virus

NMR

nuclear magnetic resonance

PAA

polyacrylamido

PBS

phosphate buffered saline

RBC

red blood cell

TLC

thin layer chromatography

TMB

3, 3’, 5, 5’-tetramethylbenzidine

VLP

virus-like particle

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jonas Nilsson
    • 1
    • 2
  • Gustaf E. Rydell
    • 1
  • Jacques Le Pendu
    • 3
  • Göran Larson
    • 1
  1. 1.Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of GothenburgSahlgrenska University HospitalGothenburgSweden
  2. 2.Department of Radiation Physics, Institute of Clinical Sciences, University of GothenburgSahlgrenska University HospitalGothenburgSweden
  3. 3.INSERM, U892Université de NantesNantesFrance

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