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Glycoconjugate Journal

, Volume 26, Issue 3, pp 301–311 | Cite as

Survey of O-GlcNAc level variations in Xenopus laevis from oogenesis to early development

  • Vanessa Dehennaut
  • Tony Lefebvre
  • Yves Leroy
  • Jean-Pierre Vilain
  • Jean-Claude Michalski
  • Jean-François Bodart
Article

Abstract

Little is known about the impact of O-linked-N-acetylglucosaminylation (O-GlcNAc) in gametes production and developmental processes. Here we investigated changes in O-GlcNAc, UDP-GlcNAc and O-GlcNAc transferase (OGT) levels in Xenopus laevis from oogenesis to embryo hatching. We showed that in comparison to stage VI, stages I–V oocytes expressed higher levels of O-GlcNAc correlating changes in OGT expression, but not in UDP-GlcNAc pools. Upon progesterone stimulation, an O-GlcNAc level burst occurred during meiotic resumption long before MPF and Mos-Erk2 pathways activations. Finally, we observed high levels of O-GlcNAc, UDP-GlcNAc and OGT during segmentation that decreased concomitantly at the onset of gastrulation. Nevertheless, no correlation between the glycosylation, the nucleotide-sugar and the glycosyltransferase was observed after neurulation. Our results show that O-GlcNAc is regulated throughout oogenesis and development within a complex pattern and suggest that dysfunctions in the dynamics of this glycosylation could lead to developmental abnormalities.

Keywords

O-GlcNAc OGT Xenopus laevis Oogenesis Meiosis Embryogenesis 

Notes

Acknowledgements

This work was granted by the “Centre National de la Recherche Scientifique”, the “Association pour la Recherche contre le Cancer” and the “Université des Sciences et Technologie de Lille I”. We thank Arlette Lescuyer-Rousseau for technical assistance. VD is a recipient of a fellowship from the “Ministère de la Recherche et de l’Enseignement”.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Vanessa Dehennaut
    • 1
    • 2
  • Tony Lefebvre
    • 1
  • Yves Leroy
    • 1
  • Jean-Pierre Vilain
    • 2
  • Jean-Claude Michalski
    • 1
  • Jean-François Bodart
    • 2
    • 3
  1. 1.UMR-CNRS 8576, Unité de Glycobiologie Structurale et FonctionnelleVilleneuve d’AscqFrance
  2. 2.Laboratoire de Régulation des Signaux de DivisionVilleneuve d’Ascq CedexFrance
  3. 3.Institut de Recherches Interdisciplinaires CNRS-USR 3078groupe des nanosystèmes biologiques, USTLVilleneuve d’Ascq cedexFrance

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