Glycoconjugate Journal

, Volume 25, Issue 9, pp 863–877

Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica)

  • Youmie Park
  • Zhenqing Zhang
  • Tatiana N. Laremore
  • Boyangzi Li
  • Joon-Soo Sim
  • A-Rang Im
  • Mi Young Ahn
  • Yeong Shik Kim
  • Robert J. Linhardt
Article

DOI: 10.1007/s10719-008-9149-1

Cite this article as:
Park, Y., Zhang, Z., Laremore, T.N. et al. Glycoconj J (2008) 25: 863. doi:10.1007/s10719-008-9149-1

Abstract

Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide →4)-α-d-GlcNpAc (1→4)-α-l-IdoAp2S(1→, analyzed by SAX (strong-anion exchange)–HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex2–4-HexNAc2), high mannose (Hex5–9-HexNAc2), and complex (Hex3-HexNAc2–10) types. None showed core fucosylation.

Keywords

Achatina fulica eggs African giant snails Neutral N-glycans Acharan sulfate Monosaccharide composition analysis Mass spectrometry 

Abbreviations

PNGase F

peptide N-glycosidase F

MS

mass spectrometry

ESI

electrospray ionization

MALDI-TOF

matrix-assisted laser desorption/ionization—time of flight

SAX

strong anion exchange

DHB

2,5-dihydroxybenzoic acid

2-PA

2-pyridylamination

GlcNAc

N-acetylglucosamine

GalNAc

N-acetylgalactosamine

CID

collision induced dissociation

Hex

hexose

HexNAc

N-acetylhexosamine

lacdiNAc

GalNAc β1–4 GlcNAc

IdoA

iduronic acid

GCC-SPE

graphitized carbon column—solid phase extraction

GAG

glycosaminoglycan

HPAEC-PAD

high-pressure anion-exchange chromatography with pulsed amperometric detection

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Youmie Park
    • 1
  • Zhenqing Zhang
    • 1
  • Tatiana N. Laremore
    • 1
  • Boyangzi Li
    • 1
  • Joon-Soo Sim
    • 2
    • 3
  • A-Rang Im
    • 2
  • Mi Young Ahn
    • 4
  • Yeong Shik Kim
    • 2
  • Robert J. Linhardt
    • 1
  1. 1.Departments of Chemistry and Chemical Biology, Biology, and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  2. 2.Natural Products Research Institute, College of PharmacySeoul National UniversityGwanak-GuRepublic of Korea
  3. 3.National Institute of Agricultural BiotechnologySuwonRepublic of Korea
  4. 4.Department of Agricultural BiologyNational Institute of Agricultural Science and TechnologySuwonRepublic of Korea

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